The gene was cloned using B. indica subsp. indica strain NBRC 3744, and DNA primers for the PCR ...The gene was cloned using B. indica subsp. indica strain NBRC 3744, and DNA primers for the PCR amplification was designed using the sequence of Bind_2021 of B. indica subsp. indica strain ATCC 9039. Two amino acid residues are different between the strain NBRC 3744 (Pro479 and Gly495) and the strain ATCC 9039 (Thr479 and Arg495). Sequence DDBJ accession number LC522529.
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 1.74 Å3/Da / 溶媒含有率: 29.26 %
結晶化
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8 詳細: 30% (w/v) polyethylene glycol 10000, 0.2 M MgCl2, 0.1 M Na K tartrate, 0.1 M Tris-HCl
解像度: 2.2→44.3 Å / Cor.coef. Fo:Fc: 0.922 / Cor.coef. Fo:Fc free: 0.906 / 交差検証法: THROUGHOUT / ESU R: 0.45 / ESU R Free: 0.261 / 詳細: Hydrogens have been added in their riding positions