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- PDB-6lym: Crystal structure of D657A mutant of formylglycinamidine synthetase -
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Open data
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Basic information
Entry | Database: PDB / ID: 6lym | ||||||
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Title | Crystal structure of D657A mutant of formylglycinamidine synthetase | ||||||
![]() | Phosphoribosylformylglycinamidine synthase | ||||||
![]() | LIGASE / Formylglycinamidine synthetase | ||||||
Function / homology | ![]() phosphoribosylformylglycinamidine synthase / phosphoribosylformylglycinamidine synthase activity / purine nucleotide biosynthetic process / 'de novo' IMP biosynthetic process / glutamine metabolic process / ATP binding / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Sharma, N. / Tanwar, A.S. / Anand, R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Mechanism of Coordinated Gating and Signal Transduction in Purine Biosynthetic Enzyme Formylglycinamidine Synthetase. Authors: Sharma, N. / Singh, S. / Tanwar, A.S. / Mondal, J. / Anand, R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 277.8 KB | Display | ![]() |
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PDB format | ![]() | 212.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 832.7 KB | Display | ![]() |
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Full document | ![]() | 846.8 KB | Display | |
Data in XML | ![]() | 49.2 KB | Display | |
Data in CIF | ![]() | 71.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6lykC ![]() 6lylC ![]() 6lyoC ![]() 7dw7C ![]() 1t3tS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 142522.406 Da / Num. of mol.: 1 / Mutation: D657A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: LT2 / SGSC1412 / ATCC 700720 / Gene: purL, STM2565 / Production host: ![]() ![]() References: UniProt: P74881, phosphoribosylformylglycinamidine synthase |
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-Non-polymers , 6 types, 478 molecules ![](data/chem/img/ADP.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/MG.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ADP / | ||||||||
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#3: Chemical | #4: Chemical | ChemComp-SO4 / #5: Chemical | ChemComp-GOL / | #6: Chemical | ChemComp-EDO / | #7: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.16 Å3/Da / Density % sol: 61.08 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / Details: 2M Ammonium Sulphate |
-Data collection
Diffraction | Mean temperature: 120 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: May 15, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5417 Å / Relative weight: 1 |
Reflection | Resolution: 2.46→50 Å / Num. obs: 59677 / % possible obs: 94.6 % / Redundancy: 6 % / Rrim(I) all: 0.155 / Net I/σ(I): 13.6 |
Reflection shell | Resolution: 2.46→2.5 Å / Num. unique obs: 3022 / CC1/2: 0.861 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1T3T Resolution: 2.46→48.21 Å / Cor.coef. Fo:Fc: 0.953 / Cor.coef. Fo:Fc free: 0.917 / SU B: 7.053 / SU ML: 0.155 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.335 / ESU R Free: 0.233 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 100.54 Å2 / Biso mean: 24.077 Å2 / Biso min: 6.17 Å2
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Refinement step | Cycle: final / Resolution: 2.46→48.21 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.461→2.525 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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