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- PDB-6lxf: Aromatic interactions drive the coupled folding and binding of th... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6lxf | |||||||||
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Title | Aromatic interactions drive the coupled folding and binding of the intrinsically disordered Sesbania mosaic virus VPg protein. | |||||||||
![]() | Polyprotein | |||||||||
![]() | PLANT VIRAL PROTEIN / IDPs (Intrinsically Disorder Proteins) / VPg (Viral Protein genome linked) | |||||||||
Function / homology | ![]() host cell membrane / viral process / serine-type endopeptidase activity / proteolysis / membrane Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | SOLUTION NMR / na | |||||||||
![]() | Dixit, K. / Karanth, N.M. / Nair, S. / Kumari, K. / Chakrabarti, K.S. / Savithri, H.S. / Sarma, S.P. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Aromatic Interactions Drive the Coupled Folding and Binding of the Intrinsically Disordered Sesbania mosaic Virus VPg Protein. Authors: Dixit, K. / Karanth, N.M. / Nair, S. / Kumari, K. / Chakrabarti, K.S. / Savithri, H.S. / Sarma, S.P. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 514.7 KB | Display | ![]() |
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PDB format | ![]() | 439.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 6568.417 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 0.3 mM [U-13C; U-15N; U-2H] SELECTIVELY 1H (CH3) ILE, VAL, LEU Sesbania Mosaic Virus- Viral Protein genome linked (SeMV VPg), 90% H2O/10% D2O Details: 0.3 mM [U-13C; U-15N; U-2H] SELECTIVELY 1H (CH3) ILE, VAL, LEU VIRAL PROTEIN GENOME LINKED (VPG) FROM SESBANIA MOSAIC VIRUS Label: U-13C, 15N, 2H selectively 1H (CH3) ILV / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 0.3 mM Component: Sesbania Mosaic Virus- Viral Protein genome linked (SeMV VPg) Isotopic labeling: [U-13C; U-15N; U-2H] SELECTIVELY 1H (CH3) ILE, VAL, LEU |
Sample conditions | Ionic strength: 0.065 Not defined / Label: condition_1 / pH: 7 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Agilent Uniform NMR System / Manufacturer: Agilent / Model: Uniform NMR System / Field strength: 600 MHz Details: Agilent (Varian) DDS2 600 MHz spectrometer equipped with a cryogenically cooled cold-probe fitted with a pulsed field gradient (z-axis only) accessory |
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Processing
NMR software |
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Refinement | Method: na / Software ordinal: 2 | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 999 / Conformers submitted total number: 25 |