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- PDB-6k5o: Development of Novel Lithocholic Acid Derivatives as Vitamin D Re... -

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Basic information

Entry
Database: PDB / ID: 6k5o
TitleDevelopment of Novel Lithocholic Acid Derivatives as Vitamin D Receptor Agonists
Components
  • Mediator of RNA polymerase II transcription subunit 1
  • Vitamin D3 receptor
KeywordsTRANSCRIPTION / VITAMIN D RECEPTOR
Function / homology
Function and homology information


positive regulation of parathyroid hormone secretion / negative regulation of bone trabecula formation / Vitamin D (calciferol) metabolism / androgen biosynthetic process / regulation of RNA biosynthetic process / SUMOylation of intracellular receptors / retinal pigment epithelium development / Nuclear Receptor transcription pathway / thyroid hormone receptor signaling pathway / response to bile acid ...positive regulation of parathyroid hormone secretion / negative regulation of bone trabecula formation / Vitamin D (calciferol) metabolism / androgen biosynthetic process / regulation of RNA biosynthetic process / SUMOylation of intracellular receptors / retinal pigment epithelium development / Nuclear Receptor transcription pathway / thyroid hormone receptor signaling pathway / response to bile acid / dense fibrillar component / cellular response to vitamin D / positive regulation of apoptotic process involved in mammary gland involution / calcitriol binding / lithocholic acid binding / nuclear receptor-mediated bile acid signaling pathway / bile acid nuclear receptor activity / positive regulation of keratinocyte differentiation / ventricular trabecula myocardium morphogenesis / vitamin D binding / mediator complex / Generic Transcription Pathway / nuclear retinoic acid receptor binding / response to aldosterone / lens development in camera-type eye / cellular response to thyroid hormone stimulus / vitamin D receptor signaling pathway / positive regulation of vitamin D receptor signaling pathway / phosphate ion transmembrane transport / megakaryocyte development / negative regulation of ossification / nuclear vitamin D receptor binding / peroxisome proliferator activated receptor binding / intestinal absorption / nuclear thyroid hormone receptor binding / cellular response to steroid hormone stimulus / negative regulation of neuron differentiation / histone acetyltransferase binding / mammary gland branching involved in pregnancy / LBD domain binding / RSV-host interactions / decidualization / regulation of calcium ion transport / nuclear receptor-mediated steroid hormone signaling pathway / negative regulation of keratinocyte proliferation / keratinocyte differentiation / general transcription initiation factor binding / positive regulation of transcription initiation by RNA polymerase II / fat cell differentiation / nuclear retinoid X receptor binding / ubiquitin ligase complex / retinoic acid receptor signaling pathway / RNA polymerase II preinitiation complex assembly / erythrocyte development / intracellular receptor signaling pathway / Regulation of lipid metabolism by PPARalpha / heterochromatin / lactation / BMAL1:CLOCK,NPAS2 activates circadian expression / RORA,B,C and NR1D1 (REV-ERBA) regulate gene expression / Activation of gene expression by SREBF (SREBP) / Expression of BMAL (ARNTL), CLOCK, and NPAS2 / positive regulation of erythrocyte differentiation / T-tubule / cellular response to epidermal growth factor stimulus / animal organ morphogenesis / nuclear estrogen receptor binding / nuclear receptor binding / skeletal system development / apoptotic signaling pathway / transcription coregulator activity / Heme signaling / positive regulation of transcription elongation by RNA polymerase II / PPARA activates gene expression / promoter-specific chromatin binding / Transcriptional activation of mitochondrial biogenesis / Cytoprotection by HMOX1 / euchromatin / Nuclear Receptor transcription pathway / protein-DNA complex / Transcriptional regulation of white adipocyte differentiation / chromatin DNA binding / response to calcium ion / caveola / mRNA transcription by RNA polymerase II / cell morphogenesis / nuclear receptor activity / RNA polymerase II transcription regulator complex / transcription coactivator binding / cellular response to amyloid-beta / intracellular calcium ion homeostasis / nuclear matrix / calcium ion transport / transcription corepressor activity / response to estradiol / ubiquitin protein ligase activity / heart development / MLL4 and MLL3 complexes regulate expression of PPARG target genes in adipogenesis and hepatic steatosis / angiogenesis / Estrogen-dependent gene expression
Similarity search - Function
: / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / Vitamin D receptor / VDR, DNA-binding domain / : / Retinoid X Receptor / Retinoid X Receptor / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. ...: / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / Vitamin D receptor / VDR, DNA-binding domain / : / Retinoid X Receptor / Retinoid X Receptor / Nuclear hormone receptor / Nuclear hormones receptors DNA-binding region signature. / Zinc finger, nuclear hormone receptor-type / Double treble clef zinc finger, C4 type / Nuclear hormone receptors DNA-binding domain profile. / c4 zinc finger in nuclear hormone receptors / Nuclear hormone receptor, ligand-binding domain / Nuclear hormone receptor-like domain superfamily / Ligand-binding domain of nuclear hormone receptor / Nuclear receptor (NR) ligand-binding (LBD) domain profile. / Ligand binding domain of hormone receptors / Zinc finger, NHR/GATA-type / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Chem-D0O / Vitamin D3 receptor / Mediator of RNA polymerase II transcription subunit 1
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.8 Å
AuthorsMasuno, H. / Kagechika, H. / Ito, N.
Funding support Japan, 3items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science15K08018 Japan
Japan Society for the Promotion of Science16K08318 Japan
Japan Society for the Promotion of Science17H03997 Japan
CitationJournal: Bioorg.Med.Chem. / Year: 2019
Title: Development of novel lithocholic acid derivatives as vitamin D receptor agonists.
Authors: Masuno, H. / Kazui, Y. / Tanatani, A. / Fujii, S. / Kawachi, E. / Ikura, T. / Ito, N. / Yamamoto, K. / Kagechika, H.
History
DepositionMay 29, 2019Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Jul 24, 2019Provider: repository / Type: Initial release
Revision 1.1Aug 14, 2019Group: Data collection / Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Nov 22, 2023Group: Data collection / Database references / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Vitamin D3 receptor
C: Mediator of RNA polymerase II transcription subunit 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)32,6213
Polymers32,1662
Non-polymers4551
Water46826
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: native gel electrophoresis
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1020 Å2
ΔGint-9 kcal/mol
Surface area11590 Å2
MethodPISA
Unit cell
Length a, b, c (Å)153.884, 43.690, 41.792
Angle α, β, γ (deg.)90.00, 96.05, 90.00
Int Tables number5
Space group name H-MC121

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Components

#1: Protein Vitamin D3 receptor / VDR / 1 / 25-dihydroxyvitamin D3 receptor / Nuclear receptor subfamily 1 group I member 1


Mass: 30595.037 Da / Num. of mol.: 1 / Mutation: 165-211 deletion
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Vdr, Nr1i1 / Production host: Escherichia coli (E. coli) / References: UniProt: P13053
#2: Protein/peptide Mediator of RNA polymerase II transcription subunit 1 / Activator-recruited cofactor 205 kDa component / ARC205 / Mediator complex subunit 1 / Peroxisome ...Activator-recruited cofactor 205 kDa component / ARC205 / Mediator complex subunit 1 / Peroxisome proliferator-activated receptor-binding protein / PPAR-binding protein / Thyroid hormone receptor-associated protein complex 220 kDa component / Trap220 / Thyroid receptor-interacting protein 2 / TRIP-2 / Vitamin D receptor-interacting protein complex component DRIP205 / p53 regulatory protein RB18A


Mass: 1570.898 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: Q15648
#3: Chemical ChemComp-D0O / (4~{R})-4-[(3~{R},5~{R},8~{R},9~{S},10~{S},13~{R},14~{S},17~{R})-10,13-dimethyl-3-methylsulfonyloxy-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1~{H}-cyclopenta[a]phenanthren-17-yl]pentanoic acid


Mass: 454.663 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C25H42O5S / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.17 Å3/Da / Density % sol: 43.36 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 0.1M MOPS/NaOH (pH 7.0), 0.1-0.4 M sodium formate, 12-22% (w/v) PEG4000, and 5% (v/v) ethylene glycol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Photon Factory / Beamline: BL-6A / Wavelength: 0.978 Å
DetectorType: ADSC QUANTUM 4r / Detector: CCD / Date: Jun 12, 2009
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.978 Å / Relative weight: 1
ReflectionResolution: 1.8→50 Å / Num. obs: 25497 / % possible obs: 98.6 % / Redundancy: 3.5 % / Rmerge(I) obs: 0.043 / Net I/σ(I): 16.3
Reflection shellResolution: 1.8→1.83 Å / Redundancy: 2.9 % / Rmerge(I) obs: 0.76 / Mean I/σ(I) obs: 1.28 / Num. unique obs: 1198 / CC1/2: 0.796 / % possible all: 91.9

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Processing

Software
NameVersionClassification
REFMAC5.8.0222refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2ZLC

2zlc


Resolution: 1.8→35.01 Å / Cor.coef. Fo:Fc: 0.965 / Cor.coef. Fo:Fc free: 0.949 / SU B: 6.727 / SU ML: 0.172 / Cross valid method: THROUGHOUT / ESU R: 0.138 / ESU R Free: 0.137 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
RfactorNum. reflection% reflectionSelection details
Rfree0.25672 1214 4.8 %RANDOM
Rwork0.21116 ---
obs0.21324 24273 98.37 %-
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å
Displacement parametersBiso mean: 41.527 Å2
Baniso -1Baniso -2Baniso -3
1--4.19 Å20 Å2-0.97 Å2
2--6.32 Å20 Å2
3----1.88 Å2
Refinement stepCycle: 1 / Resolution: 1.8→35.01 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1984 0 31 26 2041
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.010.0142059
X-RAY DIFFRACTIONr_bond_other_d0.0010.0171916
X-RAY DIFFRACTIONr_angle_refined_deg1.4061.6762789
X-RAY DIFFRACTIONr_angle_other_deg0.9281.6624510
X-RAY DIFFRACTIONr_dihedral_angle_1_deg5.685244
X-RAY DIFFRACTIONr_dihedral_angle_2_deg32.56422.941102
X-RAY DIFFRACTIONr_dihedral_angle_3_deg15.9115378
X-RAY DIFFRACTIONr_dihedral_angle_4_deg18.0331511
X-RAY DIFFRACTIONr_chiral_restr0.070.2274
X-RAY DIFFRACTIONr_gen_planes_refined0.0060.022203
X-RAY DIFFRACTIONr_gen_planes_other0.0010.02341
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it2.9574.071985
X-RAY DIFFRACTIONr_mcbond_other2.944.07984
X-RAY DIFFRACTIONr_mcangle_it4.1736.0851226
X-RAY DIFFRACTIONr_mcangle_other4.1756.0871227
X-RAY DIFFRACTIONr_scbond_it3.5964.4471074
X-RAY DIFFRACTIONr_scbond_other3.5954.4481075
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other5.4736.5051564
X-RAY DIFFRACTIONr_long_range_B_refined6.39147.9832299
X-RAY DIFFRACTIONr_long_range_B_other6.39647.9852300
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
LS refinement shellResolution: 1.799→1.845 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.423 88 -
Rwork0.384 1593 -
obs--89.89 %

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