PDB-6io4: Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A

Basic information

• Entry: Database: PDB / ID: 6io4
• Title: Silver-bound Glyceraldehyde-3-phosphate dehydrogenase A
• Components: Glyceraldehyde-3-phosphate dehydrogenase A
• Keywords: CYTOSOLIC PROTEIN / silver / dehydrogenase

Function / homology

Function:

glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) / glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity / glycolytic process / glucose metabolic process / NAD binding / NADP binding / identical protein binding / membrane / cytosol

Domain/homology:

Glyceraldehyde 3-phosphate dehydrogenase, active site / Glyceraldehyde 3-phosphate dehydrogenase active site. / Glyceraldehyde-3-phosphate dehydrogenase, type I / Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain / Glyceraldehyde 3-phosphate dehydrogenase, NAD(P) binding domain / Glyceraldehyde 3-phosphate dehydrogenase, catalytic domain / Glyceraldehyde/Erythrose phosphate dehydrogenase family / Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain / Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain / Dihydrodipicolinate Reductase; domain 2 / Dihydrodipicolinate Reductase; domain 2 / NAD(P)-binding domain superfamily / 2-Layer Sandwich / Alpha Beta

Component:

SILVER ION / Glyceraldehyde-3-phosphate dehydrogenase A

• Biological species: Escherichia coli (E. coli)
• Method: X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.1 Å
• Authors: Wang, H. / Sun, H. / Wang, M.

Funding support

Hong Kong, 1items

Organization	Grant number	Country
The University Grants Committee, Research Grants Council (RGC)	17305415P	Hong Kong

• Citation: Journal: Chem Sci / Year: 2019; Title: Antimicrobial silver targets glyceraldehyde-3-phosphate dehydrogenase in glycolysis ofE. coli.; Authors: Wang, H. / Wang, M. / Yang, X. / Xu, X. / Hao, Q. / Yan, A. / Hu, M. / Lobinski, R. / Li, H. / Sun, H.

History

Deposition	Oct 29, 2018	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Jul 3, 2019	Provider: repository / Type: Initial release
Revision 1.1	Aug 14, 2019	Group: Data collection / Database references / Category: citation Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2	Nov 20, 2019	Group: Database references / Category: citation / citation_author Item: _citation.journal_id_ISSN / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID
Revision 1.3	Nov 22, 2023	Group: Data collection / Database references / Refinement description Category: chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession

Assembly

Deposited unit

A: Glyceraldehyde-3-phosphate dehydrogenase A

B: Glyceraldehyde-3-phosphate dehydrogenase A

C: Glyceraldehyde-3-phosphate dehydrogenase A

D: Glyceraldehyde-3-phosphate dehydrogenase A

E: Glyceraldehyde-3-phosphate dehydrogenase A

F: Glyceraldehyde-3-phosphate dehydrogenase A

G: Glyceraldehyde-3-phosphate dehydrogenase A

H: Glyceraldehyde-3-phosphate dehydrogenase A

I: Glyceraldehyde-3-phosphate dehydrogenase A

J: Glyceraldehyde-3-phosphate dehydrogenase A

K: Glyceraldehyde-3-phosphate dehydrogenase A

L: Glyceraldehyde-3-phosphate dehydrogenase A

M: Glyceraldehyde-3-phosphate dehydrogenase A

N: Glyceraldehyde-3-phosphate dehydrogenase A

O: Glyceraldehyde-3-phosphate dehydrogenase A

P: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• 566 kDa, 16 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	566,320	32
Polymers	564,594	16
Non-polymers	1,726	16
Water	0	0

1

A: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• Evidence: gel filtration
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

2

B: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

3

C: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

4

D: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

5

E: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

6

F: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

7

G: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

8

H: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

9

I: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

10

J: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

11

K: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

12

L: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

13

M: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

14

N: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

15

O: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

16

P: Glyceraldehyde-3-phosphate dehydrogenase A

hetero molecules

Summary:

• defined by author
• 35.4 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	35,395	2
Polymers	35,287	1
Non-polymers	108	1
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Unit cell

Length a, b, c (Å)	87.991, 110.019, 139.712
Angle α, β, γ (deg.)	87.660, 86.940, 87.260
Int Tables number	1
Space group name H-M	P1

Components

#1: Protein

A B C D E F G H I J K L M N O P
Glyceraldehyde-3-phosphate dehydrogenase A / GAPDH-A / NAD-dependent glyceraldehyde-3-phosphate dehydrogenase

Details:

Mass: 35287.102 Da / Num. of mol.: 16 / Mutation: D78G
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (strain K12) (bacteria)
Strain: K12 / Gene: gapA, b1779, JW1768 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: P0A9B2, glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)

#2: Chemical

A-401 B-401 C-401 D-401 E-401 F-401 G-401 H-401 I-401 J-401 K-401 L-401 M-401 N-401 O-401 P-401
ChemComp-AG / SILVER ION

Details:

Mass: 107.868 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Ag

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 2.39 ų/Da / Density % sol: 48.48 %
• Crystal grow: Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7 / Details: 0.1M Malic acid, PEG3350 20%

Data collection

• Diffraction: Mean temperature: 100 K / Ambient temp details: nitrogen flow / Serial crystal experiment: N
• Diffraction source: Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.97914 Å
• Detector: Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 22, 2017
• Radiation: Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 0.97914 Å / Relative weight: 1
• Reflection: Resolution: 3.1→139.41 Å / Num. obs: 85684 / % possible obs: 90.4 % / Redundancy: 3.6 % / CC_1/2: 0.996 / R_merge(I) obs: 0.089 / R_pim(I) all: 0.055 / R_rim(I) all: 0.105 / Net I/σ(I): 8.5 / Num. measured all: 306417

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	Rmerge(I) obs	Num. measured all	Num. unique obs	CC1/2	Rpim(I) all	Rrim(I) all	Net I/σ(I) obs	% possible all
3.1-3.18	3.6	0.56	23970	6570	0.818	0.338	0.655	2.1	93.1
13.86-139.41	3.4	0.052	3252	967	0.994	0.032	0.061	18.2	91.2

Processing

Software

Name	Version	Classification
PHENIX	(1.14_3260:001)	refinement
Aimless	0.5.32	data scaling
PDB_EXTRACT	3.24	data extraction
xia2		data reduction
PHASER	2.7.2	phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 1GAD

1gad

Resolution: 3.1→87.773 Å / SU ML: 0.43 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 28.83 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.2397	4129	4.83 %
Rwork	0.1747	81440	-
obs	0.1779	85569	90.25 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
• Displacement parameters: B_iso max: 219.97 Ų / B_iso mean: 94.9022 Ų / B_iso min: 42.11 Ų

Refinement step

Cycle: final / Resolution: 3.1→87.773 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	38625	0	16	0	38641
Biso mean	-	-	111.54	-	-
Num. residues	-	-	-	-	5264

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 29

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Num. reflection all	% reflection obs (%)
3.1-3.1365	0.3394	161	0.2846	2944	3105	93
3.1365-3.1747	0.3398	155	0.2586	2854	3009	92
3.1747-3.2149	0.3527	137	0.249	2885	3022	93
3.2149-3.2572	0.3269	124	0.2529	2925	3049	92
3.2572-3.3019	0.3839	130	0.2466	2834	2964	92
3.3019-3.349	0.3506	155	0.2402	2845	3000	90
3.349-3.399	0.3105	134	0.2314	2796	2930	91
3.399-3.4521	0.3369	131	0.2197	2756	2887	89
3.4521-3.5087	0.2866	120	0.2163	2799	2919	87
3.5087-3.5692	0.3467	127	0.2077	2592	2719	85
3.5692-3.6341	0.2997	99	0.225	2393	2492	76
3.6341-3.704	0.2829	123	0.2142	2643	2766	84
3.704-3.7796	0.2789	146	0.2074	2957	3103	96
3.7796-3.8618	0.2935	149	0.1976	2971	3120	96
3.8618-3.9517	0.2523	145	0.1945	2996	3141	96
3.9517-4.0505	0.2452	178	0.1716	2913	3091	95
4.0505-4.16	0.2277	163	0.1651	2938	3101	94
4.16-4.2824	0.2804	149	0.1551	2891	3040	94
4.2824-4.4206	0.2139	178	0.1455	2942	3120	94
4.4206-4.5786	0.1811	164	0.1405	2856	3020	93
4.5786-4.7619	0.2152	139	0.1402	2865	3004	92
4.7619-4.9786	0.1977	127	0.1455	2838	2965	91
4.9786-5.2411	0.1953	180	0.1444	2799	2979	92
5.2411-5.5694	0.2531	156	0.162	2835	2991	91
5.5694-5.9993	0.2344	113	0.1771	2726	2839	87
5.9993-6.6029	0.2751	122	0.1765	2631	2753	85
6.6029-7.5579	0.2206	111	0.1622	2372	2483	76
7.5579-9.5203	0.1592	173	0.14	2863	3036	93
9.5203-87.8085	0.1954	140	0.148	2781	2921	89

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.6615	0.2338	1.169	0.9887	-1.405	3.0765	0.0706	-0.1337	0.173	0.0356	0.3403	-0.1167	0.2772	0.9244	-0.4966	1.0966	0.098	0.2288	1.0169	-0.243	0.7307	98.7637	-22.015	-64.6188
2	8.9337	-2.0681	1.2091	2.2954	0.5461	2.0958	0.2105	1.268	1.0863	-0.7901	-0.0814	-0.4594	-0.336	0.7363	-0.2674	1.1608	0.1294	0.4849	0.8847	-0.0619	0.7598	97.2695	-14.3725	-74.8918
3	5.0225	-0.8819	4.0707	4.3667	0.2727	3.5289	0.1024	-0.1867	0.9148	-0.0621	-0.2134	-0.066	-0.0478	0.7037	0.1916	1.3439	0.0042	0.3461	0.6562	0.0147	0.8366	85.273	-9.4933	-74.5654
4	3.1012	1.2862	-2.1373	2.8918	-0.6483	4.645	0.3555	0.6992	-0.0746	-0.5027	-0.1766	0.0853	-0.2163	-0.5125	-0.193	1.0815	0.1937	0.056	0.8033	-0.1513	0.5611	75.6034	-23.7998	-72.3141
5	1.6142	-1.0387	-1.7214	0.755	1.0296	2.0887	0.1078	0.4309	0.1207	-0.6816	0.2432	-0.0618	-0.4322	-0.1135	-0.2818	0.881	0.0964	-0.0118	0.5836	-0.0652	0.492	74.0861	-17.3701	-52.3177
6	1.5594	-0.2525	-0.4314	1.1696	1.1492	2.4102	0.1159	0.299	-0.2967	-0.0684	-0.1512	0.1383	0.4862	-0.3041	0.0468	0.9036	0.0634	0.0058	0.6592	-0.152	0.51	72.9969	-31.2383	-58.881
7	3.9074	-0.4754	-2.0764	0.5179	0.8972	2.3292	-0.3969	0.0548	0.0905	0.3901	0.3111	-0.1881	0.6309	0.036	0.0329	0.9533	0.0825	0.0351	0.3251	-0.069	0.7021	72.4451	-25.9971	-15.4434
8	4.2211	-0.146	-2.2212	3.2878	2.5354	3.5788	0.4296	-0.2037	0.5088	-0.1017	0.0157	-0.3576	0.0813	0.1708	-0.3871	1.1419	0.1467	0.0338	0.35	-0.1602	0.6727	77.677	-21.9734	-11.407
9	0.778	-0.2819	-0.9649	0.757	0.1486	2.8463	-0.1256	0.1611	-0.2485	0.2247	-0.0538	0.2561	0.7481	-0.3318	0.1802	0.9921	-0.1312	0.1102	0.4867	-0.1721	0.6816	62.8495	-34.017	-29.7145
10	4.1666	0.8202	0.2723	3.1297	0.9667	4.4782	0.0208	0.2281	0.1846	-0.1076	-0.2331	0.235	-0.1941	-0.5719	0.1751	0.661	0.1003	0.0656	0.4749	-0.1229	0.4793	61.466	-18.736	-42.7254
11	3.8942	2.658	1.6407	6.2635	-2.5009	3.7233	0.2162	0.266	0.1303	0.3262	-0.2985	0.7405	-0.6096	-0.3769	0.0905	0.999	0.0931	0.1262	0.4114	-0.191	0.7162	58.9937	-24.7587	-21.6385
12	5.3228	-1.164	0.6942	7.2454	-0.5711	4.6612	0.3551	0.9447	-0.368	-0.6842	-0.361	0.5442	-0.1039	0.7094	0.1649	0.7364	0.04	-0.1204	0.6293	-0.064	0.4937	6.6238	-37.7677	4.1783
13	4.2844	-0.2238	-1.7111	3.5227	-2.7437	5.657	0.1638	0.3709	-0.2614	-0.7122	0.1942	-0.1578	0.455	-0.567	-0.3271	1.2413	0.2352	-0.1056	0.8198	-0.0319	0.7192	1.3344	-40.8116	6.2118
14	7.583	-0.1028	5.3206	0.1629	0.6076	6.6861	-0.2027	0.0868	-0.7146	-0.0387	0.3416	0.0937	-0.0208	0.1041	-0.21	0.9226	0.2356	0.0196	0.7986	0.0537	0.7186	-2.1086	-35.715	10.7664
15	2.3066	-1.382	0.9894	1.9248	-1.2319	3.0226	-0.2473	0.5217	-0.6928	-0.951	0.167	0.8281	-0.2281	-0.3975	0.0795	1.1254	0.2809	-0.1604	0.9278	-0.1845	0.7868	2.0701	-46.8574	-2.5475
16	0.0943	-0.0312	-0.2108	1.8722	-0.1908	4.828	0.619	0.6487	-0.7266	-1.3461	-0.0013	-0.5203	2.0087	0.8547	-0.7177	1.8308	0.3388	-0.1018	0.8461	-0.2369	0.8534	13.9823	-51.9171	-3.1602
17	4.3969	0.3491	0.6907	2.4073	-0.0174	3.9852	0.4188	1.2356	-0.3187	-1.0125	0.1454	-0.4401	1.5487	0.6619	-0.1834	1.2112	0.5953	-0.0051	1.0843	-0.1346	0.6028	20.9695	-41.1817	-6.6087
18	4.9635	1.5554	2.1083	5.8888	0.678	0.8896	-0.0066	0.8488	0.1254	-0.5241	0.0639	-0.1768	-0.2289	0.106	-0.2534	1.1223	0.2346	0.1684	0.824	-0.0005	0.6	28.4115	-32.463	0.6539
19	1.5392	-0.8754	0.9502	0.5559	-0.5078	2.5239	0.2515	0.3215	-0.0121	-0.8062	0.0211	-0.2691	0.6016	0.5136	-0.1858	1.0217	0.2692	0.1618	0.6423	-0.0202	0.62	27.9912	-42.0758	16.5682
20	3.3024	-0.2873	0.0433	1.4291	-2.0866	3.092	0.3202	0.6735	0.5261	-0.2122	-0.1724	-0.0761	-0.238	0.0377	-0.1876	0.9171	0.2238	0.1903	0.6241	0.0803	0.6127	32.4606	-32.2345	9.9722
21	4.5142	1.4915	-1.146	2.5413	-1.124	3.8628	0.2216	0.5566	0.9519	-0.7945	-0.1108	-0.2314	-1.1541	0.3014	0.1788	1.06	0.1881	0.0424	0.6538	0.2747	0.8021	24.5219	-19.3882	6.9639
22	5.2499	0.5264	1.4863	1.709	1.4709	3.8468	0.1473	0.6149	0.1435	-0.8411	-0.3247	-0.2221	-0.5623	0.3417	0.2002	0.9637	0.194	0.1528	0.6128	0.151	0.5642	24.341	-27.8684	7.3067
23	4.7819	1.1057	2.0808	1.3953	-0.8354	2.4241	-0.0222	-0.1859	0.0109	0.2066	0.1239	-0.134	-0.4223	-0.0375	-0.0708	0.796	0.1458	-0.0104	0.545	-0.13	0.5569	32.7452	-31.8379	51.315
24	2.7883	0.8531	-0.4783	3.901	-1.493	5.4542	0.1185	-0.3945	0.8355	0.6856	-0.0534	-0.1984	-1.2754	0.2313	-0.065	0.8165	-0.0586	-0.092	0.5595	-0.1627	0.7622	42.9568	-22.1903	50.0293
25	2.2534	0.0323	0.4038	1.0185	-0.61	2.7461	0.0972	0.2296	0.373	-0.1427	-0.1677	-0.1925	-0.2456	0.3904	0.0525	0.6629	0.0087	0.1095	0.4538	0.0381	0.63	40.0646	-30.1027	25.0972
26	3.4061	-1.1911	-0.6132	3.5346	0.1134	5.4143	0.1303	0.1828	-0.2695	-0.3745	-0.1806	-0.2771	0.6543	0.5557	0.074	0.6606	0.1355	0.1066	0.5091	-0.0213	0.631	41.5544	-43.796	26.8024
27	1.1427	-0.3708	0.9524	3.0064	1.923	5.7308	-0.0247	0.5437	0.2474	-0.1192	-0.1741	0.0045	0.2507	0.6733	0.0463	0.6457	0.0681	0.1049	0.5725	0.0771	0.6098	43.3471	-34.2622	35.941
28	0.8243	0.4491	-0.9997	2.884	-1.3248	3.5567	-0.0836	0.0026	0.4079	0.1695	0.2538	0.7518	-0.5345	-0.726	-0.1398	1.0739	0.2677	0.1934	0.7173	-0.0665	1.0803	39.2338	36.7067	7.009
29	0.2361	0.4028	0.4493	2.4218	0.0084	1.0694	-0.065	0.0317	0.2087	-0.2218	0.0991	0.0501	-0.542	-0.3617	0.0822	1.6836	0.3308	0.4198	-0.0483	-0.1297	0.9548	61.696	43.476	-0.2336
30	6.362	2.0546	0.8883	6.4658	-0.5522	7.7467	0.0077	0.3301	-0.3239	0.0721	0.2592	-0.335	-0.7881	0.5872	-0.0569	0.7261	-0.1541	0.0362	0.4825	-0.1348	0.6717	91.564	14.9971	-4.4581
31	2.0898	1.0863	-1.536	1.8356	-0.467	3.3728	-0.3372	0.3093	-0.7281	0.0157	-0.2076	-0.5178	0.123	0.1615	0.3925	0.9562	0.0545	0.0873	0.5697	-0.3159	1.0404	92.7214	10.5736	0.1271
32	6.8428	-0.6821	1.2584	0.9729	-0.9859	1.0475	-0.1695	0.8172	-0.6253	0.0475	0.1901	-0.1603	-0.2471	0.9625	-0.0377	1.0192	0.0764	0.1102	0.6769	-0.2735	0.8013	86.9167	5.0529	-0.6431
33	2.9739	2.5862	-1.5328	6.838	0.7689	2.0018	0.2182	0.3622	-1.1453	0.8172	0.0344	-1.6376	-0.3606	0.8945	-0.0731	0.8711	-0.1289	-0.091	0.7338	-0.2596	1.2151	102.2818	11.8339	0.8063
34	1.3573	2.3756	-0.1565	4.6958	-1.0012	2.0697	0.0368	-0.5327	0.028	0.3726	-0.195	-0.5479	-1.1085	0.7071	0.1423	0.9095	-0.3917	0.077	0.7632	-0.2961	1.0565	100.6379	27.9605	-1.3161
35	4.8539	-0.8659	0.4498	1.1238	1.6287	3.0495	0.8098	0.1447	-0.7234	-0.9369	0.2909	0.1348	-1.0199	0.6934	-0.4878	1.3606	-0.3248	0.6547	0.5146	-0.2657	1.5001	95.6995	31.4313	-12.5
36	0.7841	0.6179	-0.1368	1.6435	-0.8835	0.5413	0.3773	0.118	0.2366	-0.5016	-0.1744	-0.6027	-0.1023	0.0483	0.2098	1.6481	-0.1129	0.2956	0.2885	-0.0646	0.9813	80.2351	39.3552	-14.6669
37	0.6204	-0.4339	-0.6896	1.3696	-0.0277	1.3137	-0.0052	0.0611	0.314	-0.3484	0.0902	-0.3212	-0.2672	0.2693	-0.1516	1.3067	-0.1549	0.2553	0.4011	-0.1087	0.946	77.5278	35.8142	-4.7526
38	1.2962	-0.5513	2.128	4.5086	-0.3684	6.1603	0.5097	0.7407	-0.053	-1.507	-0.0261	-0.7322	0.6351	0.4776	-0.3642	1.1436	0.0677	0.3027	0.4956	-0.0962	0.869	75.2349	26.9318	-20.9507
39	1.8896	0.4896	0.6183	0.4807	0.6649	1.134	0.1608	0.2351	0.2872	-0.3102	0.175	-0.1757	-0.6498	0.0787	-0.1913	1.3606	-0.0224	0.376	0.3712	-0.0862	0.9354	79.363	28.8446	-13.5207
40	3.4236	-0.0917	-0.1559	1.2001	0.2822	3.6299	0.3546	0.1362	-0.6317	-0.4122	0.2419	-0.0156	0.2993	-0.5708	-0.4849	0.8947	-0.0745	-0.1181	0.4939	0.0601	0.7622	112.5543	-59.8436	35.995
41	3.4264	-0.2414	0.3641	1.4463	0.8066	4.3153	0.1876	0.4359	-1.449	-0.3539	0.131	-0.3266	1.3387	-0.7035	-0.5157	1.0715	-0.1608	-0.3038	0.655	-0.0684	0.8203	114.5325	-69.3896	30.9119
42	3.2964	0.5948	-0.5431	2.2394	-0.0917	2.879	0.3092	-0.2699	-1.0216	-0.1001	0.1867	0.6558	1.1139	-1.2866	-0.4099	1.0733	-0.4558	-0.3769	1.0193	0.206	1.0541	95.864	-68.8199	39.3361
43	2.0613	-3.2615	-2.225	6.3703	5.2038	6.6653	-0.0446	-0.9835	-0.7252	0.3649	0.6855	0.079	0.851	-0.8099	-0.335	0.5647	-0.2284	-0.0154	1.5776	0.5166	1.0317	90.5582	-53.7162	49.0645
44	0.687	0.0684	0.6047	3.6481	-1.9688	1.6585	0.3246	-0.6327	-0.383	0.1806	0.0634	0.5781	0.0368	-0.951	-0.3242	0.6114	0.0285	0.0002	1.0692	0.164	0.6193	90.6789	-45.0399	32.7043
45	0.8963	-0.8148	0.8667	1.109	-0.4022	1.2633	0.0252	-0.5417	-0.5862	-0.0108	0.4113	0.4645	0.3524	-1.2367	-0.4605	0.5314	-0.0676	-0.1021	1.2009	0.2529	0.6704	89.5771	-46.4594	37.6158
46	3.6344	-0.2983	-1.7858	1.1173	-0.2499	2.511	0.2949	-1.2425	0.0098	0.4894	0.3817	0.4389	-0.385	-1.1517	-0.6454	0.8305	0.115	0.1398	1.4163	0.1767	0.6307	92.5956	-42.5455	56.2623
47	3.3417	-0.505	-1.5723	1.7723	0.1106	2.9555	0.1884	-0.7031	-0.2879	0.3948	0.3291	0.3495	-0.3153	-1.0409	-0.4571	0.5026	-0.0002	-0.0367	1.0761	0.2492	0.5464	97.3482	-47.659	48.5369
48	2.1056	0.522	0.2505	2.7078	0.6526	3.0921	-0.3857	-0.223	0.2403	-0.1778	0.0224	0.1412	-0.9954	-0.5854	0.376	1.359	0.4487	0.0256	0.7791	-0.09	0.8484	96.7966	-8.3578	25.1488
49	5.6892	-1.4726	-1.7793	7.2982	2.9934	5.8134	-0.0166	0.2769	0.846	-0.935	-0.404	-0.7615	-0.7012	-0.292	0.4722	1.1973	0.2223	-0.0106	0.6281	0.0702	0.8199	100.9122	-7.5022	18.6463
50	3.2464	-0.8342	-3.4834	2.5944	-0.9049	5.1417	-0.0929	-0.8338	0.7085	0.3329	0.0704	-0.0474	-1.2705	-0.0644	0.1257	1.6056	0.6607	-0.1678	1.2643	-0.3628	0.8382	92.1726	-2.2854	38.7553
51	1.9169	-1.8985	-0.9949	8.9791	1.5219	4.0499	0.2227	-0.343	0.3731	-0.3284	-0.0179	0.1139	-0.1322	-1.0057	0.2291	1.3234	0.8997	0.127	1.6045	-0.0986	0.6166	79.726	-11.4121	38.2705
52	1.5195	-0.0626	-1.3873	0.5043	-0.4401	1.7519	0.3283	-0.9357	0.0875	0.1912	0.4911	0.5907	-0.8896	-1.5762	-0.6193	0.9915	0.4289	0.1921	2.0691	0.3067	0.7199	77.7309	-28.6115	40.4846
53	0.5502	-0.5774	-0.4585	1.5414	0.1763	0.4791	0.1728	-0.8176	0.4885	0.2434	0.0897	0.1023	-0.887	-0.9646	-0.3605	0.9947	0.353	0.1513	1.2168	-0.0741	0.5752	95.3001	-27.0432	42.7332
54	1.2013	-0.5664	-1.6165	1.536	1.0162	2.2239	-0.2264	-0.8499	-0.7042	-0.1497	0.1142	0.5765	0.2118	-2.1309	0.0003	0.614	0.2148	0.0041	2.0275	0.3951	0.8692	73.4196	-35.1152	36.0694
55	4.9794	-0.1185	-2.0241	2.0005	1.192	1.8372	0.4331	-0.0224	0.1562	-0.3511	-0.0506	0.5133	-0.3481	-1.5294	-0.322	0.4984	0.1825	-0.0364	1.1915	0.0936	0.669	79.9722	-32.4212	28.697
56	3.1764	0.2381	1.0451	9.3265	1.4208	0.6962	-0.4659	-0.9909	-0.0322	-0.2268	0.761	0.5033	-0.7954	-0.9842	-0.199	0.9905	0.582	0.1238	1.2348	0.0669	0.5834	83.7168	-20.155	32.0948
57	0.4934	-0.7831	0.9505	2.4375	-1.5468	1.8632	-0.2234	-0.0345	-0.4399	0.3829	0.2745	-0.3331	1.238	0.6651	0.0446	1.994	0.5554	0.0612	0.9396	-0.1939	0.8087	93.0584	-50.4372	-45.0895
58	6.587	2.3058	-2.0224	1.1681	-0.1587	1.4493	0.4871	-0.4904	-0.9728	-0.9342	-0.2212	-1.1579	2.144	1.2574	-0.0922	2.211	0.8406	0.1541	1.4086	0.018	0.9342	97.8441	-59.6362	-34.1216
59	0.8835	0.4852	0.5621	2.7282	0.7972	2.0101	0.0021	-0.216	-0.2597	0.3308	0.2855	-0.2355	1.1267	0.9806	-0.1988	1.7274	0.9932	-0.1509	1.9158	-0.367	0.7517	109.7604	-45.5701	-30.3981
60	1.2123	0.1862	0.5098	1.8957	-0.3117	0.6849	-0.0775	-0.1284	-0.1107	0.3025	0.698	-0.2077	0.7647	1.6722	-0.0842	1.1552	0.5682	-0.0439	1.3777	-0.4443	0.548	100.7532	-28.7909	-27.9518
61	1.3828	0.729	1.0107	1.6665	0.4917	0.736	-0.0394	0.5945	0.4052	-0.1479	0.7113	-0.4926	0.0267	2.1656	-0.3795	0.8227	0.1941	0.0757	2.283	-0.5368	1.0502	119.0199	-22.05	-37.5975
62	4.5243	0.2493	-0.8334	0.3389	-0.0822	0.1586	-0.475	0.0994	-0.2014	0.1987	0.7561	-0.6007	0.5891	1.5622	-0.212	0.9017	0.2994	0.0989	1.5896	-0.3758	0.7012	109.6548	-28.892	-46.0463
63	0.1974	0.2063	0.308	0.9837	0.0222	1.1251	0.1378	-0.2136	-0.1814	0.0866	0.1088	-0.1508	0.7201	1.2609	-0.2461	1.0858	0.5353	-0.0363	1.7164	-0.5209	0.7322	110.4378	-31.759	-38.468
64	4.5962	0.5929	0.7714	2.6354	2.5775	4.4452	0.2105	0.2795	0.3966	-0.2372	-0.0622	-0.2061	-0.3117	0.5352	-0.0828	0.9745	-0.0456	0.1922	0.5007	-0.0743	0.6393	79.5637	2.1991	-35.098
65	5.3213	-0.227	2.6021	9.7891	1.3761	5.7303	0.2069	1.2847	0.6265	-0.5143	-0.1005	0.4644	-1.2923	0.7382	-0.1197	1.1899	-0.0257	0.108	0.6731	0.0927	0.5234	75.8283	10.9499	-40.7168
66	2.3001	-0.2275	-0.5218	0.8066	0.9373	1.4475	0.1645	-0.0421	0.4043	-0.3861	0.2229	-0.4188	-0.5092	1.057	-0.4325	0.9777	-0.3051	0.2357	0.9771	-0.2256	0.7517	100.0086	0.0618	-34.4729
67	0.9076	0.6037	1.1505	1.643	1.4663	4.8514	0.1448	0.0821	0.0298	0.1898	0.3846	-0.5077	0.0139	1.2617	-0.4269	0.7206	0.0204	0.0234	1.012	-0.2982	0.6599	99.306	-12.9042	-21.1655
68	2.4772	-0.2945	-0.038	2.4652	-2.021	1.6982	-0.2921	-0.2602	0.3351	0.5364	0.9485	-0.1797	-0.0347	1.0061	-0.1199	0.8511	0.0632	0.1002	0.9999	-0.4294	0.497	98.3844	-7.1395	-23.8161
69	2.8702	0.3164	-2.5751	4.105	-1.5086	2.9263	-0.0168	-0.5654	0.1256	0.0969	0.7086	0.5363	0.4458	-2.0653	-0.7847	0.9751	-0.1455	-0.0598	1.7865	0.4112	0.8163	37.1963	16.5561	-38.5742
70	3.6123	0.625	1.0832	2.0662	0.2058	0.8114	0.0711	-0.9956	0.007	0.488	-0.6643	0.3987	0.8404	-0.703	0.0744	1.0937	-0.2365	-0.0781	1.8078	0.3355	0.8321	27.9302	16.9193	-30.7989
71	1.6707	0.8299	-0.0663	0.9335	0.7464	1.1679	0.4548	-0.5432	0.1855	0.8986	-0.7665	0.2062	0.4517	-1.1652	0.2075	1.1022	0.0915	0.0491	2.314	0.0461	0.9299	28.5554	27.8973	-27.411
72	1.444	1.5933	-0.5548	2.2243	0.1518	1.3827	-0.2507	-1.0724	-0.0655	0.3295	-0.0128	-0.0601	-0.7739	-0.645	0.1016	1.2319	0.2602	-0.0904	2.0039	0.1074	0.7192	41.6628	39.6278	-31.0779
73	0.8253	-0.7915	0.8272	0.8543	-1.1238	1.9404	-0.2606	-0.6871	0.0647	0.7384	0.6689	0.1063	-0.1819	-0.268	-0.2477	1.0112	0.1352	-0.0012	1.3286	0.0847	0.6562	35.8469	41.5637	-51.2737
74	2.1498	-0.9499	0.9601	1.8172	-1.6255	2.2947	-0.0317	-1.1679	0.1451	0.8672	0.4404	-0.2197	-0.4685	-0.7035	-0.2767	1.0568	0.0646	0.0532	1.2327	0.0095	0.5629	39.4357	43.1753	-47.6903
75	1.8523	-0.5736	0.238	3.323	-0.6279	0.573	-0.4953	-0.9158	0.6804	0.4404	0.4429	-1.1503	-0.8421	-0.1017	0.1092	1.162	-0.1921	-0.4248	1.0836	-0.2005	0.7391	57.8866	48.4044	-43.4511
76	1.8495	-1.006	1.1841	2.3897	-0.7945	2.5396	-0.1929	-0.931	-0.1659	0.5126	0.4097	-0.6958	-0.6027	-0.0247	-0.0873	1.0204	-0.2408	-0.1981	1.1714	-0.0492	0.744	56.3974	38.563	-46.1647
77	1.2925	-0.7293	0.286	1.3624	-0.9259	2.9363	-0.1986	-0.7689	-0.1539	0.5018	0.6031	0.163	-0.6391	-0.9123	-0.27	1.0717	-0.0167	-0.2757	1.4667	0.168	0.6198	46.5873	34.8782	-38.2499
78	2.5983	0.4208	1.5477	5.3253	0.4279	3.818	0.218	0.1884	-0.2543	-0.3886	-0.0485	-0.8912	0.1295	0.4761	-0.1588	0.6455	0.008	0.1115	0.5321	-0.0332	0.7452	50.4083	45.3318	-87.0288
79	1.4578	0.4482	0.6308	1.8437	-0.9612	3.2602	0.0258	-0.3795	0.0741	0.2454	-0.1536	-0.4657	-0.6623	0.1642	0.1229	0.7424	-0.1805	-0.0804	0.8241	-0.0378	0.5454	50.0022	48.8458	-61.8045
80	4.2064	0.9033	-0.1038	7.2276	0.7165	5.5944	0.1065	-0.5519	0.2333	0.764	-0.0315	0.4677	-1.2102	-1.6723	-0.1949	0.847	0.2272	0.0319	0.8738	-0.0832	0.5356	33.122	58.3431	-63.68
81	5.1902	-0.0262	-1.9548	4.4243	0.716	6.3956	-0.3293	-0.6449	0.2701	0.9174	0.5004	-0.1497	-0.3798	-0.6297	-0.1629	0.7037	0.1407	-0.0761	0.646	-0.0232	0.5623	40.7337	52.6004	-67.9974
82	1.7472	-0.2357	-1.0805	0.4599	0.5228	0.998	0.0581	0.2456	0.3005	0.0573	0.1058	-0.7767	0.6047	-0.2942	-0.2339	0.9243	-0.1662	-0.1939	0.9571	0.2473	1.2981	63.915	13.8093	-59.6935
83	4.1207	1.7557	-3.2317	1.9879	0.0057	4.1349	0.5223	-0.1026	-0.7839	-0.1216	0.4775	-0.1314	0.881	0.5668	-0.8773	0.8865	0.1871	-0.3495	1.0379	0.0042	1.1669	67.6062	18.9893	-58.8347
84	5.3002	-0.5519	-1.027	4.4886	0.036	3.3767	0.0519	-1.1277	-1.0153	-0.2514	0.467	-0.153	0.3385	0.2268	-0.5381	0.7792	0.0082	-0.1851	0.8116	0.1765	1.1342	69.2221	20.3317	-57.2023
85	3.6998	2.6697	0.9693	2.7095	1.9718	2.4099	-0.0454	0.6843	-0.7363	-0.0764	0.2796	-2.2394	1.3481	1.2415	-0.4281	1.2865	0.3132	0.0426	0.825	-0.1402	1.6388	71.86	9.342	-71.4807
86	0.8838	2.6365	0.2468	8.8711	-0.7712	5.9275	0.4442	-0.0089	-0.259	-1.8913	0.2897	0.1615	1.9469	0.6138	-0.6188	1.4139	0.175	-0.4171	0.8146	0.0427	1.1884	61.3864	0.7992	-71.2237
87	0.4736	0.8168	-0.2046	1.8861	-0.3953	0.0851	0.3238	-0.1921	-0.7811	-1.0055	0.1223	0.364	1.3487	-0.1365	-0.211	1.6051	-0.2175	-0.5149	0.7038	0.323	1.3198	42.257	6.2993	-69.5388
88	4.4479	5.2109	0.3578	8.0022	2.1119	1.5387	0.5279	-1.0882	-1.3858	-0.4206	-0.3284	0.158	1.6078	-0.8787	-0.2565	1.264	-0.4932	-0.4296	1.2517	0.7611	1.7519	37.9205	4.4189	-60.8279
89	1.7294	0.7604	-0.4392	0.3711	-0.4962	2.4601	0.6413	0.1598	-1.0245	-0.2941	0.0542	0.3755	1.9354	0.0469	-0.6113	1.6989	0.0382	-0.5564	0.7926	0.2956	1.408	51.7028	4.5944	-63.3605
90	1.1391	1.5664	0.9251	2.1519	1.2718	0.7505	0.2404	-1.4223	-1.2633	0.3499	0.5206	0.6563	0.2093	-1.769	-0.8125	0.6141	-0.3488	-0.1653	1.5391	0.4934	1.1284	14.1527	34.8646	-70.512
91	6.812	1.0915	0.014	4.5282	0.4591	1.0336	-0.3349	-0.8759	-0.1586	0.3672	0.7097	1.2703	0.1901	-1.1146	-0.3342	0.884	-0.0328	0.0028	1.5812	0.5659	1.0015	14.7855	42.5241	-67.2094
92	6.1625	-0.9156	2.6398	3.9939	0.2011	1.2913	0.0615	-1.4688	-0.3326	0.9684	0.4905	1.4754	0.5606	-0.9863	-0.3955	1.1301	-0.1456	0.1538	2.0828	0.8408	1.6515	4.1618	30.7216	-62.3265
93	0.6948	0.2861	-0.6166	0.1919	-0.2966	0.5704	0.1421	-0.3901	-0.1184	0.4282	-0.4855	0.3251	0.1736	-0.0021	-0.3878	0.8822	-0.5488	-0.0486	2.0094	0.8272	1.7305	0.7353	24.7677	-64.9734
94	2.0134	0.503	0.4302	1.2098	-0.4601	0.8371	0.367	0.0211	-0.3728	-0.3526	0.5216	0.7686	0.7619	-1.3063	-0.2428	1.3056	-0.7935	-0.5421	1.5807	0.6955	1.7253	12.4106	15.3411	-76.3883
95	2.1645	0.6775	0.9735	1.0887	0.7206	0.901	0.5522	-0.5248	-0.5626	-0.1499	0.6102	0.5268	0.8748	-0.5742	0.0706	1.3381	-0.6627	-0.4417	1.3177	0.8065	1.5214	28.0511	15.1431	-63.1528
96	1.7416	0.506	-0.6672	0.6547	-0.286	0.2715	-0.1686	0.0992	0.0238	-0.6213	0.4183	1.4077	0.2404	-0.6551	-0.2496	1.3287	-0.587	-0.3491	1.2056	0.5919	1.7447	23.9024	11.593	-68.1645
97	0.6818	0.147	-0.0356	0.0907	0.0911	0.1321	0.3311	-0.2776	-0.6541	-0.1348	0.124	0.0754	0.2688	-0.0113	-0.1111	1.3822	-0.5814	-0.5909	0.8537	0.3478	1.1796	29.5607	11.1297	-75.0589
98	2.232	4.2665	2.4312	8.1717	4.8002	4.4643	0.1986	0.3494	-0.1853	-0.3512	0.5632	0.0416	1.2301	-0.81	-0.7558	1.2365	-0.403	-0.4189	0.6608	0.1776	0.9983	28.6169	17.4453	-89.1863
99	1.0723	0.5068	-0.7966	0.2528	-0.2203	2.133	0.2358	-0.1098	-0.686	-0.6508	0.1	0.2604	1.165	-0.3933	-0.2834	1.1798	-0.3836	-0.583	0.8069	0.1992	1.1822	31.6269	16.9319	-82.7004
100	5.1342	-1.3475	-1.1615	0.4459	0.6005	1.1804	0.8752	-0.5532	-0.6829	-0.5246	0.0978	0.4413	1.0169	-0.8514	-0.7611	1.1015	-0.4972	-0.4964	1.298	0.5663	1.264	19.514	21.7867	-77.5445
101	1.6024	0.1659	0.1947	1.4578	-1.637	1.9446	0.1977	-0.1743	0.1548	0.5812	-0.1062	-0.2218	-0.732	0.0466	-0.0846	1.3584	-0.0348	0.0958	0.5621	-0.2948	0.8442	74.2618	35.3249	29.9304
102	2.4707	1.9745	1.1167	4.0852	-0.4122	1.2006	0.2959	-0.2684	-0.1622	1.7315	-0.3595	-0.5596	-0.2655	0.6144	0.0895	1.379	-0.0609	-0.2578	0.6762	-0.1497	0.8194	82.5987	24.8791	39.2899
103	5.5768	0.2489	-0.3751	4.9512	-0.0899	4.471	-0.57	-1.0494	0.3393	1.2788	0.022	0.013	0.0383	-0.1632	0.4638	1.484	0.0899	0.0106	0.6562	-0.1659	0.6178	73.7172	15.7866	40.9224
104	0.8746	-0.4317	-0.6305	0.655	-0.0308	1.874	-0.0847	-0.2187	0.0039	0.4206	0.1915	0.0605	0.238	-0.0592	-0.0844	1.1615	0.0015	0.1538	0.4681	-0.1133	0.6555	62.1965	9.8802	25.5615
105	2.9881	1.5715	-1.2206	4.8355	1.4048	3.962	-0.0174	-0.0259	0.2143	0.2445	0.2658	0.3661	-0.2311	-0.1275	-0.1882	0.9904	0.0605	0.0738	0.3743	-0.0021	0.7122	58.2764	6.6485	-17.1029
106	1.6148	2.1558	-0.9962	3.8479	-0.3015	1.6356	0.1882	0.4369	0.3053	0.0173	0.1516	0.3101	-0.4497	-0.346	-0.3166	0.8238	0.145	0.0603	0.4085	0.0257	0.7203	52.6169	5.5885	-18.934
107	4.0231	0.5975	0.1418	0.7217	1.4642	4.0068	0.1723	0.0385	-0.0424	0.6432	-0.3593	0.423	0.9556	-0.3278	0.104	0.9183	-0.1495	0.0766	0.3915	-0.0207	0.6878	50.134	-8.617	-6.5879
108	2.4235	2.6305	-0.791	5.5982	-1.4043	0.9653	-0.3702	0.217	-0.1224	0.2415	0.6875	0.3324	0.6399	-0.1497	-0.2033	1.2915	-0.0391	0.0909	0.3154	-0.0354	0.6756	61.0872	-7.7408	10.2271
109	1.5815	-0.3058	-0.9603	0.1237	-0.3033	3.954	0.1394	-0.1037	0.1481	0.3715	0.0931	0.2775	0.0388	-0.4373	-0.1994	0.913	-0.0358	0.1478	0.4445	-0.0875	0.7573	52.4391	7.7765	7.9428
110	2.2712	-0.4107	-0.8534	2.0674	0.7101	3.3361	0.124	-0.1306	-0.0119	0.2111	0.0406	-0.0184	0.3848	0.1932	-0.1682	1.0294	0.0151	-0.0075	0.2976	-0.102	0.6501	69.0331	-3.9962	9.6638
111	2.9435	-0.4007	2.0493	1.2086	-1.5413	2.8031	-0.0944	0.1092	-0.0076	0.7422	-0.3211	0.3211	0.2778	-0.0208	0.274	0.9091	-0.0348	0.1132	0.3163	-0.0857	0.6856	62.0115	-3.6855	-1.7746

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 66 )	A	1 - 66
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 67 through 94 )	A	67 - 94
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 95 through 111 )	A	95 - 111
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 112 through 164 )	A	112 - 164
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 165 through 196 )	A	165 - 196
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 197 through 330 )	A	197 - 330
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 1 through 45 )	B	1 - 45
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 46 through 74 )	B	46 - 74
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 75 through 224 )	B	75 - 224
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 225 through 314 )	B	225 - 314
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 315 through 330 )	B	315 - 330
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 1 through 21 )	C	1 - 21
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 22 through 45 )	C	22 - 45
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 46 through 66 )	C	46 - 66
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 67 through 94 )	C	67 - 94
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 95 through 111 )	C	95 - 111
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 112 through 142 )	C	112 - 142
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 143 through 164 )	C	143 - 164
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 165 through 196 )	C	165 - 196
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 197 through 263 )	C	197 - 263
21	X-RAY DIFFRACTION	21	chain 'C' and (resid 264 through 279 )	C	264 - 279
22	X-RAY DIFFRACTION	22	chain 'C' and (resid 280 through 330 )	C	280 - 330
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 1 through 56 )	D	1 - 56
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 57 through 142 )	D	57 - 142
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 143 through 263 )	D	143 - 263
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 264 through 303 )	D	264 - 303
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 304 through 330 )	D	304 - 330
28	X-RAY DIFFRACTION	28	chain 'E' and (resid 1 through 142 )	E	1 - 142
29	X-RAY DIFFRACTION	29	chain 'E' and (resid 143 through 330 )	E	143 - 330
30	X-RAY DIFFRACTION	30	chain 'F' and (resid 1 through 21 )	F	1 - 21
31	X-RAY DIFFRACTION	31	chain 'F' and (resid 22 through 45 )	F	22 - 45
32	X-RAY DIFFRACTION	32	chain 'F' and (resid 46 through 63 )	F	46 - 63
33	X-RAY DIFFRACTION	33	chain 'F' and (resid 64 through 90 )	F	64 - 90
34	X-RAY DIFFRACTION	34	chain 'F' and (resid 91 through 128 )	F	91 - 128
35	X-RAY DIFFRACTION	35	chain 'F' and (resid 129 through 150 )	F	129 - 150
36	X-RAY DIFFRACTION	36	chain 'F' and (resid 151 through 176 )	F	151 - 176
37	X-RAY DIFFRACTION	37	chain 'F' and (resid 177 through 263 )	F	177 - 263
38	X-RAY DIFFRACTION	38	chain 'F' and (resid 264 through 279 )	F	264 - 279
39	X-RAY DIFFRACTION	39	chain 'F' and (resid 280 through 330 )	F	280 - 330
40	X-RAY DIFFRACTION	40	chain 'G' and (resid 1 through 56 )	G	1 - 56
41	X-RAY DIFFRACTION	41	chain 'G' and (resid 57 through 83 )	G	57 - 83
42	X-RAY DIFFRACTION	42	chain 'G' and (resid 84 through 142 )	G	84 - 142
43	X-RAY DIFFRACTION	43	chain 'G' and (resid 143 through 164 )	G	143 - 164
44	X-RAY DIFFRACTION	44	chain 'G' and (resid 165 through 196 )	G	165 - 196
45	X-RAY DIFFRACTION	45	chain 'G' and (resid 197 through 237 )	G	197 - 237
46	X-RAY DIFFRACTION	46	chain 'G' and (resid 238 through 279 )	G	238 - 279
47	X-RAY DIFFRACTION	47	chain 'G' and (resid 280 through 330 )	G	280 - 330
48	X-RAY DIFFRACTION	48	chain 'H' and (resid 1 through 45 )	H	1 - 45
49	X-RAY DIFFRACTION	49	chain 'H' and (resid 46 through 74 )	H	46 - 74
50	X-RAY DIFFRACTION	50	chain 'H' and (resid 75 through 128 )	H	75 - 128
51	X-RAY DIFFRACTION	51	chain 'H' and (resid 129 through 150 )	H	129 - 150
52	X-RAY DIFFRACTION	52	chain 'H' and (resid 151 through 176 )	H	151 - 176
53	X-RAY DIFFRACTION	53	chain 'H' and (resid 177 through 237 )	H	177 - 237
54	X-RAY DIFFRACTION	54	chain 'H' and (resid 238 through 263 )	H	238 - 263
55	X-RAY DIFFRACTION	55	chain 'H' and (resid 264 through 303 )	H	264 - 303
56	X-RAY DIFFRACTION	56	chain 'H' and (resid 304 through 330 )	H	304 - 330
57	X-RAY DIFFRACTION	57	chain 'I' and (resid 1 through 74 )	I	1 - 74
58	X-RAY DIFFRACTION	58	chain 'I' and (resid 75 through 94 )	I	75 - 94
59	X-RAY DIFFRACTION	59	chain 'I' and (resid 95 through 164 )	I	95 - 164
60	X-RAY DIFFRACTION	60	chain 'I' and (resid 165 through 237 )	I	165 - 237
61	X-RAY DIFFRACTION	61	chain 'I' and (resid 238 through 263 )	I	238 - 263
62	X-RAY DIFFRACTION	62	chain 'I' and (resid 264 through 293 )	I	264 - 293
63	X-RAY DIFFRACTION	63	chain 'I' and (resid 294 through 330 )	I	294 - 330
64	X-RAY DIFFRACTION	64	chain 'J' and (resid 1 through 56 )	J	1 - 56
65	X-RAY DIFFRACTION	65	chain 'J' and (resid 57 through 82 )	J	57 - 82
66	X-RAY DIFFRACTION	66	chain 'J' and (resid 83 through 224 )	J	83 - 224
67	X-RAY DIFFRACTION	67	chain 'J' and (resid 225 through 293 )	J	225 - 293
68	X-RAY DIFFRACTION	68	chain 'J' and (resid 294 through 330 )	J	294 - 330
69	X-RAY DIFFRACTION	69	chain 'K' and (resid 1 through 66 )	K	1 - 66
70	X-RAY DIFFRACTION	70	chain 'K' and (resid 67 through 83 )	K	67 - 83
71	X-RAY DIFFRACTION	71	chain 'K' and (resid 84 through 111 )	K	84 - 111
72	X-RAY DIFFRACTION	72	chain 'K' and (resid 112 through 164 )	K	112 - 164
73	X-RAY DIFFRACTION	73	chain 'K' and (resid 165 through 196 )	K	165 - 196
74	X-RAY DIFFRACTION	74	chain 'K' and (resid 197 through 237 )	K	197 - 237
75	X-RAY DIFFRACTION	75	chain 'K' and (resid 238 through 264 )	K	238 - 264
76	X-RAY DIFFRACTION	76	chain 'K' and (resid 265 through 303 )	K	265 - 303
77	X-RAY DIFFRACTION	77	chain 'K' and (resid 304 through 330 )	K	304 - 330
78	X-RAY DIFFRACTION	78	chain 'L' and (resid 1 through 148 )	L	1 - 148
79	X-RAY DIFFRACTION	79	chain 'L' and (resid 149 through 251 )	L	149 - 251
80	X-RAY DIFFRACTION	80	chain 'L' and (resid 252 through 280 )	L	252 - 280
81	X-RAY DIFFRACTION	81	chain 'L' and (resid 281 through 330 )	L	281 - 330
82	X-RAY DIFFRACTION	82	chain 'M' and (resid 1 through 21 )	M	1 - 21
83	X-RAY DIFFRACTION	83	chain 'M' and (resid 22 through 45 )	M	22 - 45
84	X-RAY DIFFRACTION	84	chain 'M' and (resid 46 through 83 )	M	46 - 83
85	X-RAY DIFFRACTION	85	chain 'M' and (resid 84 through 111 )	M	84 - 111
86	X-RAY DIFFRACTION	86	chain 'M' and (resid 112 through 150 )	M	112 - 150
87	X-RAY DIFFRACTION	87	chain 'M' and (resid 151 through 280 )	M	151 - 280
88	X-RAY DIFFRACTION	88	chain 'M' and (resid 281 through 303 )	M	281 - 303
89	X-RAY DIFFRACTION	89	chain 'M' and (resid 304 through 330 )	M	304 - 330
90	X-RAY DIFFRACTION	90	chain 'N' and (resid 1 through 21 )	N	1 - 21
91	X-RAY DIFFRACTION	91	chain 'N' and (resid 22 through 74 )	N	22 - 74
92	X-RAY DIFFRACTION	92	chain 'N' and (resid 75 through 101 )	N	75 - 101
93	X-RAY DIFFRACTION	93	chain 'N' and (resid 102 through 118 )	N	102 - 118
94	X-RAY DIFFRACTION	94	chain 'N' and (resid 119 through 164 )	N	119 - 164
95	X-RAY DIFFRACTION	95	chain 'N' and (resid 165 through 196 )	N	165 - 196
96	X-RAY DIFFRACTION	96	chain 'N' and (resid 197 through 224 )	N	197 - 224
97	X-RAY DIFFRACTION	97	chain 'N' and (resid 225 through 251 )	N	225 - 251
98	X-RAY DIFFRACTION	98	chain 'N' and (resid 252 through 280 )	N	252 - 280
99	X-RAY DIFFRACTION	99	chain 'N' and (resid 281 through 303 )	N	281 - 303
100	X-RAY DIFFRACTION	100	chain 'N' and (resid 304 through 330 )	N	304 - 330
101	X-RAY DIFFRACTION	101	chain 'O' and (resid 1 through 83 )	O	1 - 83
102	X-RAY DIFFRACTION	102	chain 'O' and (resid 84 through 110 )	O	84 - 110
103	X-RAY DIFFRACTION	103	chain 'O' and (resid 111 through 148 )	O	111 - 148
104	X-RAY DIFFRACTION	104	chain 'O' and (resid 149 through 330 )	O	149 - 330
105	X-RAY DIFFRACTION	105	chain 'P' and (resid 1 through 45 )	P	1 - 45
106	X-RAY DIFFRACTION	106	chain 'P' and (resid 46 through 110 )	P	46 - 110
107	X-RAY DIFFRACTION	107	chain 'P' and (resid 111 through 148 )	P	111 - 148
108	X-RAY DIFFRACTION	108	chain 'P' and (resid 149 through 176 )	P	149 - 176
109	X-RAY DIFFRACTION	109	chain 'P' and (resid 177 through 224 )	P	177 - 224
110	X-RAY DIFFRACTION	110	chain 'P' and (resid 225 through 303 )	P	225 - 303
111	X-RAY DIFFRACTION	111	chain 'P' and (resid 304 through 330 )	P	304 - 330