- PDB-6igc: Crystal structure of HPV58/33/52 chimeric L1 pentamer -
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基本情報
登録情報
データベース: PDB / ID: 6igc
タイトル
Crystal structure of HPV58/33/52 chimeric L1 pentamer
要素
Major capsid protein L1
キーワード
STRUCTURAL PROTEIN / capsid protein
機能・相同性
機能・相同性情報
T=7 icosahedral viral capsid / endocytosis involved in viral entry into host cell / host cell nucleus / virion attachment to host cell / structural molecule activity 類似検索 - 分子機能
Major capsid L1 (late) protein, Papillomavirus / Major capsid L1 (late) superfamily, Papillomavirus / L1 (late) protein / Double-stranded DNA virus, group I, capsid 類似検索 - ドメイン・相同性
ジャーナル: Nat Commun / 年: 2018 タイトル: Rational design of a triple-type human papillomavirus vaccine by compromising viral-type specificity. 著者: Zhihai Li / Shuo Song / Maozhou He / Daning Wang / Jingjie Shi / Xinlin Liu / Yunbing Li / Xin Chi / Shuangping Wei / Yurou Yang / Zhiping Wang / Jinjin Li / Huilian Qian / Hai Yu / Qingbing ...著者: Zhihai Li / Shuo Song / Maozhou He / Daning Wang / Jingjie Shi / Xinlin Liu / Yunbing Li / Xin Chi / Shuangping Wei / Yurou Yang / Zhiping Wang / Jinjin Li / Huilian Qian / Hai Yu / Qingbing Zheng / Xiaodong Yan / Qinjian Zhao / Jun Zhang / Ying Gu / Shaowei Li / Ningshao Xia / 要旨: Sequence variability in surface-antigenic sites of pathogenic proteins is an important obstacle in vaccine development. Over 200 distinct genomic sequences have been identified for human ...Sequence variability in surface-antigenic sites of pathogenic proteins is an important obstacle in vaccine development. Over 200 distinct genomic sequences have been identified for human papillomavirus (HPV), of which more than 18 are associated with cervical cancer. Here, based on the high structural similarity of L1 surface loops within a group of phylogenetically close HPV types, we design a triple-type chimera of HPV33/58/52 using loop swapping. The chimeric VLPs elicit neutralization titers comparable with a mix of the three wild-type VLPs both in mice and non-human primates. This engineered region of the chimeric protein recapitulates the conformational contours of the antigenic surfaces of the parental-type proteins, offering a basis for this high immunity. Our stratagem is equally successful in developing other triplet-type chimeras (HPV16/35/31, HPV56/66/53, HPV39/68/70, HPV18/45/59), paving the way for the development of an improved HPV prophylactic vaccine against all carcinogenic HPV strains. This technique may also be extrapolated to other microbes.
#200 - 2016年8月 正二十面体型ウイルスの準対称性 (Quasisymmetry in Icosahedral Viruses) 類似性 (2)
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集合体
登録構造単位
A: Major capsid protein L1 B: Major capsid protein L1 C: Major capsid protein L1 D: Major capsid protein L1 E: Major capsid protein L1 F: Major capsid protein L1 G: Major capsid protein L1 H: Major capsid protein L1 I: Major capsid protein L1 J: Major capsid protein L1 K: Major capsid protein L1 L: Major capsid protein L1 M: Major capsid protein L1 N: Major capsid protein L1 O: Major capsid protein L1 P: Major capsid protein L1 Q: Major capsid protein L1 R: Major capsid protein L1 S: Major capsid protein L1 T: Major capsid protein L1 U: Major capsid protein L1 V: Major capsid protein L1 W: Major capsid protein L1 X: Major capsid protein L1 Y: Major capsid protein L1 Z: Major capsid protein L1 a: Major capsid protein L1 b: Major capsid protein L1 c: Major capsid protein L1 d: Major capsid protein L1 e: Major capsid protein L1 f: Major capsid protein L1 g: Major capsid protein L1 h: Major capsid protein L1 i: Major capsid protein L1 j: Major capsid protein L1 k: Major capsid protein L1 l: Major capsid protein L1 m: Major capsid protein L1 n: Major capsid protein L1