Evidence: isothermal titration calorimetry, dilution ITC does not dissociate this dimer, gel filtration, SEC-MALS data indicate that this molecule is a homodimer
Method to determine structure: SAD / Resolution: 1.5→41.39 Å / Cor.coef. Fo:Fc: 0.971 / Cor.coef. Fo:Fc free: 0.965 / SU B: 2.313 / SU ML: 0.039 / Cross valid method: THROUGHOUT / ESU R: 0.061 / ESU R Free: 0.058 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.18317
1261
4.7 %
RANDOM
Rwork
0.15233
-
-
-
obs
0.1538
25696
99.89 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å