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Yorodumi- PDB-6g5v: The X-ray structure of the adduct formed in the reaction between ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6g5v | ||||||
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Title | The X-ray structure of the adduct formed in the reaction between lysozyme and a platinum(II) terpyridine compound (pH 7.0) | ||||||
Components | Lysozyme C | ||||||
Keywords | HYDROLASE / Platinum terpyridine compounds / Protein metalation / ESI MS elucidation / X-Ray structure determination | ||||||
Function / homology | Function and homology information Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium ...Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.96 Å | ||||||
Authors | Merlino, A. / Ferraro, G. | ||||||
Citation | Journal: Dalton Trans / Year: 2018 Title: A case of extensive protein platination: the reaction of lysozyme with a Pt(ii)-terpyridine complex. Authors: Ferraro, G. / Marzo, T. / Infrasca, T. / Cilibrizzi, A. / Vilar, R. / Messori, L. / Merlino, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6g5v.cif.gz | 39.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6g5v.ent.gz | 28.9 KB | Display | PDB format |
PDBx/mmJSON format | 6g5v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g5/6g5v ftp://data.pdbj.org/pub/pdb/validation_reports/g5/6g5v | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gallus gallus (chicken) / References: UniProt: P00698, lysozyme | ||||||
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#2: Chemical | ChemComp-PT / #3: Chemical | ChemComp-DMS / | #4: Chemical | ChemComp-CL / | #5: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 38.78 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.8 M succinic acid/NaOH, 0.10 M HEPES pH 7.0, 1% MPEG 2K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU SATURN 944 / Detector: CCD / Date: Mar 16, 2016 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.96→56.18 Å / Num. obs: 8823 / % possible obs: 99.3 % / Redundancy: 2.8 % / CC1/2: 0.812 / Rmerge(I) obs: 0.084 / Rpim(I) all: 0.04 / Net I/σ(I): 8.1 |
Reflection shell | Resolution: 1.96→1.99 Å / Redundancy: 2.4 % / Rmerge(I) obs: 0.478 / Mean I/σ(I) obs: 3.1 / Rpim(I) all: 0.256 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.96→56.18 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.924 / SU B: 4.936 / SU ML: 0.141 / Cross valid method: THROUGHOUT / ESU R: 0.202 / ESU R Free: 0.183 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.567 Å2
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Refinement step | Cycle: 1 / Resolution: 1.96→56.18 Å
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Refine LS restraints |
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