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Open data
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Basic information
Entry | Database: PDB / ID: 6fvi | ||||||
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Title | ASH / PapD-like domain of human CEP192 (PapD-like domain 7) | ||||||
![]() | Centrosomal protein of 192 kDa | ||||||
![]() | CELL CYCLE / Centrosome / centriole / PapD-like domain / ASH domain | ||||||
Function / homology | ![]() centrosome-templated microtubule nucleation / procentriole / procentriole replication complex / centriole replication / protein localization to centrosome / pericentriolar material / mitotic spindle assembly / phosphatase binding / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome ...centrosome-templated microtubule nucleation / procentriole / procentriole replication complex / centriole replication / protein localization to centrosome / pericentriolar material / mitotic spindle assembly / phosphatase binding / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / centriole / AURKA Activation by TPX2 / response to bacterium / Regulation of PLK1 Activity at G2/M Transition / centrosome / protein kinase binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kovalevskiy, O.V. / van Breugel, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: ASH / PapD-like domain of human CEP192 (PapD-like domain 7) Authors: Kovalevskiy, O. / van Breugel, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 156.2 KB | Display | ![]() |
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PDB format | ![]() | 131.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 422.5 KB | Display | ![]() |
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Full document | ![]() | 424.3 KB | Display | |
Data in XML | ![]() | 10.6 KB | Display | |
Data in CIF | ![]() | 15.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 16950.646 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-CL / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.44 % |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: 100 mM Na-Citrate, pH 5.5, 30 % (v/v) PEG-400, 100 mM NaCl, 100 mM MgCl2 |
-Data collection
Diffraction | Mean temperature: 100 K | ||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||||||||
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Apr 12, 2015 | ||||||||||||||||||||||||||||||
Radiation | Protocol: MAD / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.72932 Å / Relative weight: 1 | ||||||||||||||||||||||||||||||
Reflection | Resolution: 1→23.03 Å / Num. obs: 82358 / % possible obs: 98.8 % / Redundancy: 14.8 % / CC1/2: 0.999 / Rmerge(I) obs: 0.071 / Rpim(I) all: 0.019 / Rrim(I) all: 0.074 / Net I/σ(I): 18.2 / Num. measured all: 1218883 / Scaling rejects: 90 | ||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 388.59 Å2 / Biso mean: 15.199 Å2 / Biso min: 0.53 Å2
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Refinement step | Cycle: final / Resolution: 1→43.83 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1→1.026 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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