5-(hydroxymethyl)furfural oxidase / Oxidoreductases; Acting on a sulfur group of donors; With oxygen as acceptor / oxidoreductase activity, acting on a sulfur group of donors, oxygen as acceptor / choline dehydrogenase activity / oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor / glycine betaine biosynthetic process from choline / FAD binding Similarity search - Function
Mass: 57078.086 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The sequence contains the V465T mutation (with respect to the wild type sequence) which was intentionally introduced by site-directed mutagenesis. Source: (gene. exp.) Methylovorus sp. (strain MP688) (bacteria) Strain: MP688 / Gene: MPQ_0130 / Production host: Escherichia coli (E. coli) References: UniProt: E4QP00, 5-(hydroxymethyl)furfural oxidase, Oxidoreductases; Acting on a sulfur group of donors; With oxygen as acceptor
Resolution: 1.9→72.67 Å / Cor.coef. Fo:Fc: 0.975 / Cor.coef. Fo:Fc free: 0.953 / SU B: 6.205 / SU ML: 0.162 / Cross valid method: THROUGHOUT / ESU R: 0.162 / ESU R Free: 0.156 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2311
3334
4.8 %
RANDOM
Rwork
0.17162
-
-
-
obs
0.17442
66372
99.49 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å