SerrateRNAeffectormoleculehomolog / Arsenite-resistance protein 2
Mass: 12027.658 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Residues 171-270 and 408-763 were co-expressed.Residues 271-407 were deleted Source: (gene. exp.) Homo sapiens (human) / Gene: SRRT, ARS2, ASR2 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta 2 / References: UniProt: Q9BXP5
#2: Protein
SerrateRNAeffectormoleculehomolog / Arsenite-resistance protein 2
Mass: 41169.176 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: Residues 171-270 and 408-763 were co-expressed.Residues 271-407 were deleted Source: (gene. exp.) Homo sapiens (human) / Gene: SRRT, ARS2, ASR2 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): Rosetta 2 / References: UniProt: Q9BXP5
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3 Å3/Da / Density % sol: 58.94 %
Crystal grow
Temperature: 281 K / Method: vapor diffusion, sitting drop / pH: 10.5 Details: Protein solution at 6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8, mixed 1:1 with reservoir solution containing 1.2 M sodium dihydrogen phosphate, 0.8 M ...Details: Protein solution at 6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8, mixed 1:1 with reservoir solution containing 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.2 M lithium sulphate and 0.1 M CAPS pH 10.5.
Resolution: 3.22→3.3 Å / Redundancy: 9.13 % / Mean I/σ(I) obs: 1.42 / Num. unique obs: 805 / CC1/2: 0.544 / Rsym value: 1.67 / % possible all: 99.9
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0189
refinement
XDS
datareduction
XSCALE
datascaling
CRANK2
phasing
Refinement
Method to determine structure: SIRAS / Resolution: 3.22→78.74 Å / Cor.coef. Fo:Fc: 0.864 / Cor.coef. Fo:Fc free: 0.779 / SU B: 33.469 / SU ML: 0.526 / Cross valid method: THROUGHOUT / ESU R Free: 0.611 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.32174
516
4.7 %
RANDOM
Rwork
0.28867
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obs
0.29033
10435
96.98 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å