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Yorodumi- PDB-6ee9: Cytokine-like Peptide Stress-response Peptide-1 from Manduca Sexta -
+Open data
-Basic information
Entry | Database: PDB / ID: 6ee9 | ||||||
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Title | Cytokine-like Peptide Stress-response Peptide-1 from Manduca Sexta | ||||||
Components | Stress-response Peptide-1 | ||||||
Keywords | CYTOKINE / Epidermal Growth Factor / Hemolymph Protein / Insect Immunity / Phenoloxidase | ||||||
Biological species | Manduca sexta (tobacco hornworm) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Schrag, L. | ||||||
Citation | Journal: To be published Title: NMR Solution Structure and Expression Profile of Stress Response Peptide-1: A Cytokine from Manduca sexta Authors: Schrag, L. / Cao, X. / Al Souhail, Q. / Kanost, M. / Jiang, H. / Prakash, O. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6ee9.cif.gz | 133.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6ee9.ent.gz | 94.9 KB | Display | PDB format |
PDBx/mmJSON format | 6ee9.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6ee9_validation.pdf.gz | 432.2 KB | Display | wwPDB validaton report |
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Full document | 6ee9_full_validation.pdf.gz | 519.9 KB | Display | |
Data in XML | 6ee9_validation.xml.gz | 11.8 KB | Display | |
Data in CIF | 6ee9_validation.cif.gz | 17.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ee/6ee9 ftp://data.pdbj.org/pub/pdb/validation_reports/ee/6ee9 | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 2818.129 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Manduca sexta (tobacco hornworm) |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 1.8 mM Stress-response Peptide-1, 90% H2O/10% D2O / Label: Stress-response Peptide-1 / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.8 mM / Component: Stress-response Peptide-1 / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength units: Not defined / Label: Conditions_1 / pH: 2.7 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 500 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 5 | ||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |