タイプ: solution 内容: 20 mM sodium phosphate, 50 mM sodium chloride, 0.5 mM [U-13C; U-15N] Human liver fatty acid binding protein (hFABP1), 90% H2O/10% D2O 詳細: 0.5 mM protein, 20 mM Sodium Phosphate, 50 mM NaCl, pH 5.5 Label: 15N13C_sample / 溶媒系: 90% H2O/10% D2O
試料
濃度 (mg/ml)
構成要素
Isotopic labeling
Solution-ID
20mM
sodiumphosphate
naturalabundance
1
50mM
sodiumchloride
naturalabundance
1
0.5mM
Humanliverfattyacidbindingprotein (hFABP1)
[U-13C; U-15N]
1
試料状態
詳細: 0.5 mM protein, 20 mM Sodium Phosphate, 50 mM NaCl, pH 5.5 イオン強度: 20 mM Sodium Phosphate, 50 mM NaCl mM / Label: conditions_1 / pH: 5.5 / 圧: 1 atm / 温度: 308 K
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NMR測定
NMRスペクトロメーター
タイプ
製造業者
モデル
磁場強度 (MHz)
Spectrometer-ID
Bruker AVANCE III
Bruker
AVANCEIII
600
1
Bruker AVANCE III
Bruker
AVANCEIII
800
2
-
解析
NMR software
名称
バージョン
開発者
分類
TopSpin
BrukerBiospin
collection
TopSpin
BrukerBiospin
解析
CARA
KellerandWuthrich
chemicalshiftassignment
CARA
KellerandWuthrich
peakpicking
CYANA
Guntert, MumenthalerandWuthrich
structurecalculation
UNIO10-ATNOS/CANID
2.0.2
Dr. TorstenHerrmann
structurecalculation
OPALp
Koradietal, 2000
精密化
Prime
Prime, Schrodinger, LLC, NewYork, NY, 2018
精密化
精密化
手法
ソフトェア番号
詳細
molecular dynamics
8
the structures are based on a total of 2553 NOE-derived distance constraints, 202 dihedral angle constraints, 58 distance restraints from hydrogen bonds