ATP phosphoribosyltransferase / ATP phosphoribosyltransferase activity / L-histidine biosynthetic process / magnesium ion binding / cytoplasm Similarity search - Function
Histidine biosynthesis HisG, C-terminal / HisG, C-terminal domain / ATP phosphoribosyltransferase HisG / ATP phosphoribosyltransferase, catalytic domain / ATP phosphoribosyltransferase, conserved site / ATP phosphoribosyltransferase / ATP phosphoribosyltransferase signature. / Nitrogen regulatory PII-like, alpha/beta / Nitrogen regulatory protein PII/ATP phosphoribosyltransferase, C-terminal Similarity search - Domain/homology
Journal: Biochem. J. / Year: 2018 Title: Guarding the gateway to histidine biosynthesis in plants:Medicago truncatulaATP-phosphoribosyltransferase in relaxed and tense states. Authors: Ruszkowski, M.
Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.978 Å / Relative weight: 1
Reflection
Resolution: 2.92→50 Å / Num. obs: 82460 / % possible obs: 99.1 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.067 / Rrim(I) all: 0.076 / Net I/σ(I): 15.2
Reflection shell
Resolution: 2.92→3.09 Å / Redundancy: 3.9 % / Rmerge(I) obs: 0.9 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 12719 / Rrim(I) all: 1.05 / % possible all: 95.3
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0103
refinement
XDS
datareduction
XDS
datascaling
HKL-3000
phasing
Refinement
Method to determine structure: SAD / Resolution: 2.92→39.17 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.917 / SU B: 37.306 / SU ML: 0.299 / Cross valid method: THROUGHOUT / ESU R: 0.524 / ESU R Free: 0.321 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25
1237
1.5 %
RANDOM
Rwork
0.197
-
-
-
obs
0.198
81223
99.15 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å