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Open data
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Basic information
Entry | Database: PDB / ID: 6coo | ||||||
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Title | CSP1-E1A | ||||||
![]() | Competence-stimulating peptide type 1 | ||||||
![]() | SIGNALING PROTEIN / pneumococcus / quorum sensing / signaling molecule / CSP | ||||||
Function / homology | Competence protein, ComC / COMC family / pheromone activity / establishment of competence for transformation / extracellular region / Competence-stimulating peptide type 1![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Yang, Y. | ||||||
![]() | ![]() Title: Structural Characterization of Competence-Stimulating Peptide Analogues Reveals Key Features for ComD1 and ComD2 Receptor Binding in Streptococcus pneumoniae. Authors: Yang, Y. / Cornilescu, G. / Tal-Gan, Y. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.6 KB | Display | ![]() |
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PDB format | ![]() | 80.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6cj8C ![]() 6copC ![]() 6coqC ![]() 6corC ![]() 6cosC ![]() 6cotC ![]() 6couC ![]() 6covC ![]() 6cowC C: citing same article ( |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 2190.699 Da / Num. of mol.: 1 / Fragment: UNP residues 25-41 / Mutation: E1A / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 250 mM D DPC, 1.9 mM competence stimulating peptide type 1 mutant - CSP1-E1A, 90% H2O/10% D2O Label: 1 / Solvent system: 90% H2O/10% D2O | ||||||||||||
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Sample |
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Sample conditions | Ionic strength: 0.15 M / Label: 1 / pH: 7.4 / Pressure: ambient atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 3 / Details: also torsion angle dynamics with eefx refinement | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |