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- PDB-6c5r: Crystal structure of the soluble domain of the mitochondrial calc... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6c5r | ||||||
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Title | Crystal structure of the soluble domain of the mitochondrial calcium uniporter | ||||||
![]() | calcium uniporter | ||||||
![]() | CYTOSOLIC PROTEIN | ||||||
Function / homology | uniporter activity / Calcium uniporter protein, C-terminal / MCU family / Mitochondrial calcium uniporter / mitochondrial calcium ion homeostasis / calcium channel activity / mitochondrial inner membrane / identical protein binding / Calcium uniporter protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Fan, C. / Fan, M. / Fastman, N. / Zhang, J. / Feng, L. | ||||||
![]() | ![]() Title: X-ray and cryo-EM structures of the mitochondrial calcium uniporter. Authors: Chao Fan / Minrui Fan / Benjamin J Orlando / Nathan M Fastman / Jinru Zhang / Yan Xu / Melissa G Chambers / Xiaofang Xu / Kay Perry / Maofu Liao / Liang Feng / ![]() ![]() Abstract: Mitochondrial calcium uptake is critical for regulating ATP production, intracellular calcium signalling, and cell death. This uptake is mediated by a highly selective calcium channel called the ...Mitochondrial calcium uptake is critical for regulating ATP production, intracellular calcium signalling, and cell death. This uptake is mediated by a highly selective calcium channel called the mitochondrial calcium uniporter (MCU). Here, we determined the structures of the pore-forming MCU proteins from two fungi by X-ray crystallography and single-particle cryo-electron microscopy. The stoichiometry, overall architecture, and individual subunit structure differed markedly from those described in the recent nuclear magnetic resonance structure of Caenorhabditis elegans MCU. We observed a dimer-of-dimer architecture across species and chemical environments, which was corroborated by biochemical experiments. Structural analyses and functional characterization uncovered the roles of key residues in the pore. These results reveal a new ion channel architecture, provide insights into calcium coordination, selectivity and conduction, and establish a structural framework for understanding the mechanism of mitochondrial calcium uniporter function. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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PDBx/mmCIF format | ![]() | 316.2 KB | Display | ![]() |
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PDB format | ![]() | 208.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 502.3 KB | Display | ![]() |
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Full document | ![]() | 516.8 KB | Display | |
Data in XML | ![]() | 42.4 KB | Display | |
Data in CIF | ![]() | 57.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7800C ![]() 7801C ![]() 7802C ![]() 7803C ![]() 7804C ![]() 6c5wC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 24267.676 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: CQMa 102 / Gene: MAC_01752 / Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.43 Å3/Da / Density % sol: 64.1 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 0.1 M glycine, pH 9.3, 15.5% PEG 4000, and 0.6 M NaNO3 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Mar 25, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.09608311048→50 Å / Num. obs: 47337 / % possible obs: 100 % / Redundancy: 3.4 % / Biso Wilson estimate: 97.3071351599 Å2 / Rmerge(I) obs: 0.089 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 3.1→3.17 Å / Num. unique obs: 3153 |
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Processing
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Refinement | Resolution: 3.09608311048→42.105090976 Å / SU ML: 0.448428869909 / Cross valid method: FREE R-VALUE / σ(F): 1.33787117744 / Phase error: 34.267652959 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 99.0706387757 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.09608311048→42.105090976 Å
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Refine LS restraints |
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LS refinement shell |
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