National Natural Science Foundation of China (NSFC)
11774012
中国
National Natural Science Foundation of China (NSFC)
91530321
中国
National Natural Science Foundation of China (NSFC)
中国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI93256
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI24982
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI100645
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI24755
米国
引用
ジャーナル: Angew Chem Int Ed Engl / 年: 2018 タイトル: Folding DNA into a Lipid-Conjugated Nanobarrel for Controlled Reconstitution of Membrane Proteins. 著者: Yuanchen Dong / Shuobing Chen / Shijian Zhang / Joseph Sodroski / Zhongqiang Yang / Dongsheng Liu / Youdong Mao / 要旨: Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared ...Building upon DNA origami technology, we introduce a method to reconstitute a single membrane protein into a self-assembled DNA nanobarrel that scaffolds a nanodisc-like lipid environment. Compared with the membrane-scaffolding-protein nanodisc technique, our approach gives rise to defined stoichiometry, controlled sizes, as well as enhanced stability and homogeneity in membrane protein reconstitution. We further demonstrate potential applications of the DNA nanobarrels in the structural analysis of membrane proteins.
1A: DNA (52-MER) 1B: DNA (55-MER) 1C: DNA (5'-D(*AP*AP*TP*AP*AP*CP*GP*GP*CP*TP*CP*AP*GP*AP*GP*C)-3') 1D: DNA (59-MER) 1E: DNA (51-MER) 1F: DNA (46-MER) 1G: DNA (5'-D(*TP*CP*AP*AP*CP*CP*GP*AP*GP*CP*TP*TP*GP*CP*TP*T)-3') 1H: DNA (48-MER) 1I: DNA (47-MER) 1J: DNA (5'-D(*TP*AP*TP*TP*AP*GP*CP*GP*AP*GP*AP*TP*GP*GP*TP*TP*TP*TP*AP*TP*TP*AP*CP*A)-3') 1K: DNA (46-MER) 1L: DNA (5'-D(*CP*GP*AP*CP*AP*GP*AP*AP*TP*GP*AP*AP*AP*GP*AP*G)-3') 1M: DNA (32-MER) 1O: DNA (40-MER) 1P: DNA (46-MER) 1Q: DNA (37-MER) 1R: DNA (48-MER) 1S: DNA (37-MER) 1T: DNA (5'-D(*CP*GP*CP*CP*AP*CP*CP*AP*GP*AP*TP*TP*CP*AP*TP*C)-3') 1U: DNA (40-MER) 1V: DNA (26-MER) 1W: DNA (40-MER) 1X: DNA (48-MER) 1Y: DNA (27-MER) 1Z: DNA (26-MER) 1a: DNA (47-MER) 1b: DNA (32-MER) 1c: DNA (40-MER) 1d: DNA (40-MER) 1e: DNA (26-MER) 1f: DNA (40-MER) 1g: DNA (48-MER) 1h: DNA (48-MER) 1i: DNA (48-MER) 1j: DNA (32-MER) 1k: DNA (40-MER) 1l: DNA (48-MER) 1m: DNA (5'-D(P*TP*CP*GP*AP*GP*GP*TP*GP*AP*TP*TP*CP*GP*CP*GP*T)-3') 1n: DNA (56-MER) 1o: DNA (40-MER) 1p: DNA (40-MER) 1q: DNA (48-MER) 1r: DNA (32-MER) 1s: DNA (32-MER) 1t: DNA (32-MER) 1u: DNA (48-MER) 1v: DNA (5'-D(P*CP*CP*CP*CP*CP*AP*GP*CP*TP*GP*GP*TP*CP*AP*TP*A)-3') 1w: DNA (40-MER) 1x: DNA (5'-D(P*GP*AP*CP*AP*GP*AP*TP*GP*CP*GP*TP*GP*CP*CP*AP*G)-3') 2N: DNA (48-MER) 2A: DNA (40-MER) 2B: DNA (48-MER) 2C: DNA (40-MER) 2D: DNA (29-MER) 2E: DNA (5'-D(P*CP*AP*TP*AP*AP*CP*GP*CP*AP*TP*AP*AP*AP*AP*CP*GP*AP*GP*GP*AP*GP*GP*TP*T)-3') 2F: DNA (32-MER) 2G: DNA (26-MER) 2H: DNA (40-MER) 2I: DNA (40-MER) 2J: DNA (58-MER) 2K: DNA (52-MER) 2L: DNA (40-MER) 2M: DNA (48-MER) 2O: DNA (48-MER) 2P: DNA (32-MER) 2Q: DNA (58-MER) 2R: DNA (56-MER) 2S: DNA (52-MER) 2T: DNA (37-MER) 2U: DNA (48-MER) 2V: DNA (48-MER) 2W: DNA (32-MER) 2X: DNA (32-MER) 2Y: DNA (48-MER) 2Z: DNA (48-MER) 2a: DNA (5'-D(P*TP*TP*TP*GP*TP*TP*AP*AP*AP*AP*CP*CP*GP*AP*TP*A)-3') 2b: DNA (32-MER) 2c: DNA (32-MER) 2d: DNA (40-MER) 2e: DNA (5'-D(P*CP*TP*GP*GP*CP*CP*TP*TP*CP*CP*TP*GP*TP*AP*GP*CP*CP*AP*AP*AP*AP*AP*TP*A)-3') 2f: DNA (32-MER) 2g: DNA (40-MER) 2h: DNA (32-MER) 2i: DNA (32-MER) 2j: DNA (47-MER) 2k: DNA (5'-D(P*CP*CP*AP*GP*TP*GP*CP*CP*AP*AP*AP*TP*CP*CP*GP*C)-3') 2l: DNA (40-MER) 2m: DNA (48-MER) 2n: DNA (48-MER) 2o: DNA (48-MER) 2p: DNA (56-MER) 2q: DNA (56-MER) 2r: DNA (32-MER) 2s: DNA (48-MER) 2t: DNA (37-MER) 2u: DNA (55-MER) 2v: DNA (48-MER) 2w: DNA (38-MER) 2x: DNA (32-MER) 3N: DNA (4346-MER) 3A: DNA (40-MER) 3B: DNA (40-MER) 3C: DNA (48-MER) 3D: DNA (48-MER) 3E: DNA (48-MER) 3F: DNA (56-MER) 3G: DNA (5'-D(*GP*TP*GP*CP*CP*TP*AP*AP*GP*GP*AP*TP*AP*TP*TP*C)-3') 3H: DNA (56-MER) 3I: DNA (36-MER) 3J: DNA (35-MER) 3S: DNA (32-MER) 3K: DNA (48-MER) 3L: DNA (37-MER) 3M: DNA (55-MER) 3O: DNA (48-MER) 3P: DNA (38-MER) 3Q: DNA (26-MER) 3R: DNA (32-MER)