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Open data
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Basic information
Entry | Database: PDB / ID: 6aay | ||||||
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Title | the Cas13b binary complex | ||||||
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![]() | RNA BINDING PROTEIN/RNA / Cas13b / ![]() | ||||||
Function / homology | ![]() ![]() | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Bo, Z. / Weiwei, Y. / Yangmiao, Y. / Ouyang, S.Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural insights into Cas13b-guided CRISPR RNA maturation and recognition. Authors: Zhang, B. / Ye, W.W. / Ye, Y.M. / Zhou, H. / Saeed, A.F.U.H. / Chen, J. / Lin, J.Y. / Perculija, V. / Chen, Q. / Chen, C.J. / Chang, M.X. / Choudhary, M.I. / Ouyang, S.Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 286.9 KB | Display | ![]() |
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PDB format | ![]() | 236.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 147588.750 Da / Num. of mol.: 1 / Mutation: R1177A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: HMPREF9699_02005 / Production host: ![]() ![]() ![]() |
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#2: RNA chain | Mass: 19072.414 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.8 Å3/Da / Density % sol: 56.13 % |
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 0.15 M Sodium chloride, 0.1 M Sodium citrate (pH 5.5), 22% (w/v) Polyethylene glycol 1,000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Apr 27, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.79→65.58 Å / Num. obs: 47521 / % possible obs: 99.88 % / Redundancy: 20 % / Biso Wilson estimate: 50.315 Å2 / CC1/2: 0.997 / Net I/σ(I): 12.2 |
Reflection shell | Resolution: 2.79→2.89 Å / Mean I/σ(I) obs: 1.9 / Num. unique obs: 2376 / CC1/2: 0.544 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 69.642 Å2
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Refinement step | Cycle: 1 / Resolution: 2.79→65.02 Å
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Refine LS restraints |
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