Mass: 18.015 Da / Num. of mol.: 127 / Source method: isolated from a natural source / Formula: H2O
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Details
Sequence details
The authors did not cloned this gene from the standard strain of Staphylococcus aureus, but from a ...The authors did not cloned this gene from the standard strain of Staphylococcus aureus, but from a clinically drug-resistant strain (MRSA R3708). ProRS of MRSA R3708 shows THR 230, ALA 278.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.85 Å3/Da / Density % sol: 56.92 %
Crystal grow
Temperature: 297 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 32-40% (v/v) Pentaerythritol propoxylate (5/4 PO/OH), 0.1-0.2 M KCl, 50 mM HEPES, pH 7.5
Resolution: 2.07→50 Å / Cor.coef. Fo:Fc: 0.912 / Cor.coef. Fo:Fc free: 0.894 / SU B: 10.946 / SU ML: 0.15 / Cross valid method: THROUGHOUT / ESU R: 0.22 / ESU R Free: 0.182 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.25449
2202
5.1 %
RANDOM
Rwork
0.22829
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obs
0.22963
40645
97.36 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å