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- PDB-5xt8: Magnesium bound apo structure of thymidylate kinase (form I) from... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5xt8 | ||||||
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Title | Magnesium bound apo structure of thymidylate kinase (form I) from Thermus thermophilus HB8 | ||||||
![]() | Thymidylate kinase | ||||||
![]() | TRANSFERASE / Nucleotide monophosphate kinase | ||||||
Function / homology | ![]() dUDP biosynthetic process / dTMP kinase / thymidylate kinase activity / dTDP biosynthetic process / dTTP biosynthetic process / phosphorylation / ATP binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Chaudhary, S.K. / Jeyakanthan, J. / Sekar, K. | ||||||
![]() | ![]() Title: Structural and functional roles of dynamically correlated residues in thymidylate kinase. Authors: Chaudhary, S.K. / Jeyakanthan, J. / Sekar, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 94.6 KB | Display | ![]() |
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PDB format | ![]() | 70.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454.3 KB | Display | ![]() |
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Full document | ![]() | 458.4 KB | Display | |
Data in XML | ![]() | 18.2 KB | Display | |
Data in CIF | ![]() | 26.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5x86C ![]() 5x8aC ![]() 5x8bC ![]() 5x8cC ![]() 5x8dC ![]() 5x8jC ![]() 5x8kC ![]() 5x8vC ![]() 5x98C ![]() 5x99C ![]() 5xakC ![]() 5xalC ![]() 5x7jS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 22009.543 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: HB8 / ATCC 27634 / DSM 579 / Gene: tmk, TTHA1607 Production host: ![]() ![]() References: UniProt: Q5SHX3, dTMP kinase #2: Chemical | ChemComp-MG / #3: Chemical | ChemComp-CL / #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44.8 % |
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Crystal grow | Temperature: 295 K / Method: microbatch Details: 0.2M MgCl2.6H2O, 0.1M Tris hydrochloride, 30% PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Jul 10, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.541 Å / Relative weight: 1 |
Reflection | Resolution: 2→48.87 Å / Num. obs: 26844 / % possible obs: 97.2 % / Redundancy: 10 % / CC1/2: 0.998 / Rmerge(I) obs: 0.103 / Net I/σ(I): 14 |
Reflection shell | Resolution: 2→2.05 Å |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5X7J Resolution: 2.01→48.87 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.913 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.182 / ESU R Free: 0.163 Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 95.3 Å2 / Biso mean: 23.2332 Å2 / Biso min: 9.5 Å2
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Refinement step | Cycle: LAST / Resolution: 2.01→48.87 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.01→2.062 Å / Total num. of bins used: 20
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