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- PDB-5sxu: X-ray structure of 2-bromoethanol bound to a pentameric ligand ga... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5sxu | |||||||||||||||
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Title | X-ray structure of 2-bromoethanol bound to a pentameric ligand gated ion channel (ELIC) in a desensitized state | |||||||||||||||
![]() | Gamma-aminobutyric-acid receptor subunit beta-1 | |||||||||||||||
![]() | PROTEIN TRANSPORT / ELIC / anesthetic / ethanol / desensitized state | |||||||||||||||
Function / homology | ![]() extracellular ligand-gated monoatomic ion channel activity / regulation of membrane potential / transmembrane signaling receptor activity / neuron projection / signal transduction / identical protein binding / membrane Similarity search - Function | |||||||||||||||
Biological species | ![]() | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Chen, Q. / Kinde, M. / Cohen, A. / Xu, Y. / Tang, P. | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Basis of Alcohol Inhibition of the Pentameric Ligand-Gated Ion Channel ELIC. Authors: Chen, Q. / Wells, M.M. / Tillman, T.S. / Kinde, M.N. / Cohen, A. / Xu, Y. / Tang, P. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 625.3 KB | Display | ![]() |
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PDB format | ![]() | 519.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 568 KB | Display | ![]() |
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Full document | ![]() | 619.7 KB | Display | |
Data in XML | ![]() | 109.1 KB | Display | |
Data in CIF | ![]() | 143.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5sxvC ![]() 3rquS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 10 molecules ABCDEFGHIJ
#1: Protein | Mass: 36879.000 Da / Num. of mol.: 10 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: 3937 / Gene: Dda3937_00520 / Plasmid: pET26b / Production host: ![]() ![]() |
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-Non-polymers , 5 types, 57 molecules ![](data/chem/img/BRJ.gif)
![](data/chem/img/3CN.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/3CN.gif)
![](data/chem/img/MES.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-BRJ / #3: Chemical | ChemComp-3CN / #4: Chemical | ChemComp-MES / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.07 Å3/Da / Density % sol: 69.76 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 6.1 Details: PEG 4000, ammonium sulfate, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 4, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9195 Å / Relative weight: 1 |
Reflection | Resolution: 3.07→39.61 Å / Num. obs: 109403 / % possible obs: 99.9 % / Redundancy: 36.4 % / Net I/σ(I): 19.1 |
Reflection shell | Resolution: 3.07→3.12 Å / Redundancy: 37.3 % / Mean I/σ(I) obs: 1 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3RQU Resolution: 3.1→29.927 Å / SU ML: 0.44 / Cross valid method: FREE R-VALUE / σ(F): 1.89 / Phase error: 28.51
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→29.927 Å
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Refine LS restraints |
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LS refinement shell |
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