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- PDB-5m6s: folding intermediate of spectrin R16 -

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Database: PDB / ID: 5m6s
Titlefolding intermediate of spectrin R16
KeywordsSTRUCTURAL PROTEIN / spectrin / r16
Specimen sourceEscherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /
MethodElectron microscopy (4.8 A resolution / Single particle)
AuthorsNilsson, O.B. / Nickson, A.A. / Clarke, J.
CitationNat. Struct. Mol. Biol., 2017, 24, 221-225

Nat. Struct. Mol. Biol., 2017, 24, 221-225 StrPapers
Cotranslational folding of spectrin domains via partially structured states.
Ola B Nilsson / Adrian A Nickson / Jeffrey J Hollins / Stephan Wickles / Annette Steward / Roland Beckmann / Gunnar von Heijne / Jane Clarke

DateDeposition: Oct 25, 2016 / Release: Jan 11, 2017 / Last modification: Mar 15, 2017

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Deposited unit
A: spectrin

Theoretical massNumber of molelcules
Total (without water)19,3711

TypeNameSymmetry operationNumber
identity operation1_5551
Buried area (A2)0
ΔGint (kcal/M)0
Surface area (A2)6420


#1: Polypeptide(L)spectrin

Mass: 19370.596 Da / Num. of mol.: 1
Source: (gene. exp.) Escherichia coli / bacteria / エシェリキア・コリ, 大腸菌 /

Biological process

Cellular component

Molecular function

Experimental details


EM experimentReconstruction method: SINGLE PARTICLE

Sample preparation

ComponentName: spectrin folding intermediate bound to 70s ribosome / Type: RIBOSOME
Specimen supportGrid material: COPPER / Grid type: Quantifoil R3/3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE

Electron microscopy imaging

MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELD


3D reconstructionResolution: 4.8 A / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 46067
Details: To exclude potential overfitting, the data were processed using a frequency limited refinement protocol by truncating high frequencies (low-pass filter at 8 A)
Atomic model buildingRef protocol: RIGID BODY FIT

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