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- PDB-5lm4: Structure of the thermostalilized EAAT1 cryst-II mutant in comple... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5lm4 | ||||||
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Title | Structure of the thermostalilized EAAT1 cryst-II mutant in complex with L-ASP and the allosteric inhibitor UCPH101 | ||||||
![]() | Excitatory amino acid transporter 1,Neutral amino acid transporter B(0),Excitatory amino acid transporter 1 | ||||||
![]() | TRANSPORT PROTEIN / excitatory amino acid transporter 1 / human glutamate transporter / SLC1A3 / UCPH101 | ||||||
Function / homology | ![]() Defective SLC1A3 causes episodic ataxia 6 (EA6) / auditory behavior / Astrocytic Glutamate-Glutamine Uptake And Metabolism / membrane protein complex / glutamine secretion / neurotransmitter uptake / L-glutamine import across plasma membrane / cranial nerve development / cell morphogenesis involved in neuron differentiation / glutamine transport ...Defective SLC1A3 causes episodic ataxia 6 (EA6) / auditory behavior / Astrocytic Glutamate-Glutamine Uptake And Metabolism / membrane protein complex / glutamine secretion / neurotransmitter uptake / L-glutamine import across plasma membrane / cranial nerve development / cell morphogenesis involved in neuron differentiation / glutamine transport / L-glutamine transmembrane transporter activity / L-serine transmembrane transporter activity / gamma-aminobutyric acid biosynthetic process / high-affinity L-glutamate transmembrane transporter activity / glutamate:sodium symporter activity / L-glutamate import / ligand-gated channel activity / Transport of inorganic cations/anions and amino acids/oligopeptides / L-glutamate transmembrane transport / L-glutamate transmembrane transporter activity / neutral amino acid transport / D-aspartate import across plasma membrane / amino acid transmembrane transporter activity / L-aspartate transmembrane transporter activity / Amino acid transport across the plasma membrane / L-aspartate import across plasma membrane / neutral L-amino acid transmembrane transporter activity / Glutamate Neurotransmitter Release Cycle / L-glutamate import across plasma membrane / symporter activity / transepithelial transport / intracellular sodium ion homeostasis / neurotransmitter transport / antiporter activity / cellular response to cocaine / glutamate binding / amino acid transport / RHOJ GTPase cycle / RHOQ GTPase cycle / protein homotrimerization / RHOH GTPase cycle / neuromuscular process controlling balance / transport across blood-brain barrier / response to light stimulus / RAC3 GTPase cycle / positive regulation of synaptic transmission / monoatomic ion transport / potassium ion transmembrane transport / chloride transmembrane transport / RAC1 GTPase cycle / erythrocyte differentiation / basal plasma membrane / sensory perception of sound / response to wounding / melanosome / virus receptor activity / signaling receptor activity / cytoplasmic vesicle / chemical synaptic transmission / response to xenobiotic stimulus / neuron projection / response to antibiotic / neuronal cell body / synapse / perinuclear region of cytoplasm / cell surface / extracellular exosome / membrane / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Canul-Tec, J. / Assal, R. / Legrand, P. / Reyes, N. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure and allosteric inhibition of excitatory amino acid transporter 1. Authors: Canul-Tec, J.C. / Assal, R. / Cirri, E. / Legrand, P. / Brier, S. / Chamot-Rooke, J. / Reyes, N. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 169.7 KB | Display | ![]() |
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PDB format | ![]() | 135.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 746.6 KB | Display | ![]() |
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Full document | ![]() | 752.9 KB | Display | |
Data in XML | ![]() | 15.1 KB | Display | |
Data in CIF | ![]() | 20.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5llmC ![]() 5lluC ![]() 5mjuC ![]() 2nwxS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 56460.324 Da / Num. of mol.: 1 Fragment: UNP residues 1-149,UNP residues 158-230,UNP residues 243-542 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: SLC1A3, EAAT1, GLAST, GLAST1, SLC1A5, ASCT2, M7V1, RDR, RDRC Plasmid: pcDNA3 / Cell (production host): embrionic / Cell line (production host): HEK-293F / Production host: ![]() |
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#2: Chemical | ChemComp-NA / |
#3: Chemical | ChemComp-ASP / |
#4: Chemical | ChemComp-6Z6 / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.5 Å3/Da / Density % sol: 64.6 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 8.2 Details: 28% PEG 400, 100 mM Tris pH 8.2, 50 mM Calcium chloride, 50 mM Barium Chloride PH range: 8.0 - 8.4 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: May 6, 2016 |
Radiation | Monochromator: Si (111) monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.976 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→45.82 Å / Num. all: 290672 / Num. obs: 14115 / % possible obs: 99.9 % / Redundancy: 20.6 % / Biso Wilson estimate: 121.37 Å2 / Rmerge(I) obs: 0.1 / Net I/σ(I): 15.8 |
Reflection shell | Resolution: 3.1→3.68 Å / Redundancy: 21.2 % / Rmerge(I) obs: 6.71 / Mean I/σ(I) obs: 0.8 / CC1/2: 0.434 / % possible all: 99.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2NWX Resolution: 3.1→45.8 Å / Cor.coef. Fo:Fc: 0.885 / Cor.coef. Fo:Fc free: 0.7792 / Cross valid method: THROUGHOUT / σ(F): 0 / SU Rfree Blow DPI: 0.444
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Displacement parameters | Biso mean: 111.65 Å2
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Refine analyze | Luzzati coordinate error obs: 0.43 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.1→45.8 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 3.1→3.47 Å / Total num. of bins used: 5
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Refinement TLS params. | Method: refined / Origin x: -468.133 Å / Origin y: 289.3662 Å / Origin z: 4.302 Å
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Refinement TLS group | Selection details: { B|* } |