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- PDB-5l0o: IQGAP1 calponin homology domain fragment (CHDF) mutant K161C unde... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5l0o | ||||||
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Title | IQGAP1 calponin homology domain fragment (CHDF) mutant K161C under oxidizing conditions | ||||||
![]() | Ras GTPase-activating-like protein IQGAP1 | ||||||
![]() | SIGNALING PROTEIN / Calponin homology / disulfide-bonded dimer | ||||||
Function / homology | ![]() negative regulation of dephosphorylation / mitotic actomyosin contractile ring assembly actin filament organization / podocyte development / slit diaphragm / GTPase inhibitor activity / fibroblast migration / MAP-kinase scaffold activity / S100 protein binding / Nephrin family interactions / neuron projection extension ...negative regulation of dephosphorylation / mitotic actomyosin contractile ring assembly actin filament organization / podocyte development / slit diaphragm / GTPase inhibitor activity / fibroblast migration / MAP-kinase scaffold activity / S100 protein binding / Nephrin family interactions / neuron projection extension / RHOV GTPase cycle / cortical actin cytoskeleton / cellular response to platelet-derived growth factor stimulus / RHOC GTPase cycle / RHOQ GTPase cycle / phosphatidylinositol-3,4,5-trisphosphate binding / CDC42 GTPase cycle / RHOU GTPase cycle / platelet-derived growth factor receptor signaling pathway / lateral plasma membrane / RHOA GTPase cycle / RAC2 GTPase cycle / positive regulation of protein kinase activity / RHO GTPases activate IQGAPs / fibroblast growth factor receptor signaling pathway / cellular response to epidermal growth factor stimulus / regulation of cytokine production / ruffle / regulation of mitotic cell cycle / RAC1 GTPase cycle / cellular response to calcium ion / protein serine/threonine kinase activator activity / GTPase activator activity / secretory granule membrane / actin filament / Signaling by high-kinase activity BRAF mutants / MAP2K and MAPK activation / epidermal growth factor receptor signaling pathway / cytoplasmic side of plasma membrane / small GTPase binding / cytoplasmic ribonucleoprotein granule / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Signaling by BRAF and RAF1 fusions / actin filament binding / cell migration / cell cortex / midbody / growth cone / basolateral plasma membrane / protein phosphatase binding / microtubule / positive regulation of MAPK cascade / molecular adaptor activity / calmodulin binding / ribonucleoprotein complex / cadherin binding / neuron projection / apical plasma membrane / protein domain specific binding / axon / focal adhesion / calcium ion binding / Neutrophil degranulation / protein kinase binding / signal transduction / extracellular exosome / nucleus / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Liu, J. / Worthylake, D. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The IQGAP1 N-Terminus Forms Dimers, and the Dimer Interface Is Required for Binding F-Actin and Calcium-Bound Calmodulin. Authors: Liu, J. / Kurella, V.B. / LeCour, L. / Vanagunas, T. / Worthylake, D.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 142 KB | Display | ![]() |
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PDB format | ![]() | 112.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 454.9 KB | Display | ![]() |
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Full document | ![]() | 472.3 KB | Display | |
Data in XML | ![]() | 25 KB | Display | |
Data in CIF | ![]() | 32.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3i6xSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | Dimer according to gel filtration, mutagenesis, shape complementary analysis |
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Components
#1: Protein | Mass: 22361.402 Da / Num. of mol.: 4 / Mutation: K161C Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain (production host): in Rosetta Gami 2(DE3) pLysS cells (EMD4Biosciences) References: UniProt: P46940 |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.96 Å3/Da / Density % sol: 58.44 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion / pH: 8 Details: 18% PEG 3350, 10% ethylene glycol, 10% glucose, 100mM -160mM potassium acetate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: Bruker Platinum 135 / Detector: CCD / Date: Jun 25, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.36→68.06 Å / Num. obs: 41528 / % possible obs: 98.4 % / Redundancy: 3.13 % / Rmerge(I) obs: 0.0847 / Net I/σ(I): 25.57 |
Reflection shell | Resolution: 2.36→2.46 Å / Redundancy: 1.79 % / Rmerge(I) obs: 0.3504 / Mean I/σ(I) obs: 2.14 / % possible all: 94.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3I6X Resolution: 2.36→15 Å / Data cutoff low absF: 0 / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 2.36→15 Å
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