- PDB-5ki0: NMR structure of human antimicrobial peptide KAMP-19 -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 5ki0
Title
NMR structure of human antimicrobial peptide KAMP-19
Components
Antimicrobial peptide KAMP-19
Keywords
ANTIMICROBIAL PROTEIN / Keratin 6A fragment / non-alpha beta structure
Function / homology
Function and homology information
: / keratin filament / Keratinization / negative regulation of entry of bacterium into host cell / Formation of the cornified envelope / cytolysis by host of symbiont cells / morphogenesis of an epithelium / wound healing / structural constituent of cytoskeleton / antimicrobial humoral immune response mediated by antimicrobial peptide ...: / keratin filament / Keratinization / negative regulation of entry of bacterium into host cell / Formation of the cornified envelope / cytolysis by host of symbiont cells / morphogenesis of an epithelium / wound healing / structural constituent of cytoskeleton / antimicrobial humoral immune response mediated by antimicrobial peptide / cell differentiation / defense response to Gram-positive bacterium / positive regulation of cell population proliferation / extracellular exosome / membrane / nucleus / cytosol Similarity search - Function
Keratin, type II / Keratin type II head / Keratin type II head / Intermediate filament protein, conserved site / Intermediate filament protein / Intermediate filament (IF) rod domain signature. / Intermediate filament, rod domain / Intermediate filament (IF) rod domain profile. / Intermediate filament protein Similarity search - Domain/homology
Mass: 1768.971 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human) / References: UniProt: P02538*PLUS
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D NOESY
1
2
1
isotropic
1
2D DQF-COSY
1
3
1
isotropic
1
2D 1H-1H TOCSY
1
4
1
isotropic
1
2D 1H-15N HSQC
1
5
1
isotropic
1
2D 1H-13C HSQC
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Sample preparation
Details
Type: micelle / Contents: 3.4 mM KAMP-19, 90% H2O/10% D2O Details: The peptide was solubilized with deuterated SDS at a peptide/SDS molar ratio of 1:60, pH 5.4 Label: 1H sample / Solvent system: 90% H2O/10% D2O
Details: The peptide was solubilized with deuterated SDS at a peptide/SDS molar ratio of 1:60, Ionic strength: nd Not defined / Ionic strength err: 0.2 / Label: condition_1 / pH: 5.4 / PH err: 0.1 / Pressure: 1 atm / Pressure err: 0.01 / Temperature: 298 K / Temperature err: 0.2
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