[English] 日本語
![](img/lk-miru.gif)
- PDB-5kfs: Human DNA polymerase eta R61A-DNA ternary complex: ground state a... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 5kfs | ||||||
---|---|---|---|---|---|---|---|
Title | Human DNA polymerase eta R61A-DNA ternary complex: ground state at pH7.0 (K+ MES) with 1 Ca2+ ion | ||||||
![]() |
| ||||||
![]() | replication / transferase/DNA / in crystallo reaction / DNA polymerase / metal ion dependent catalysis / transferase-DNA complex | ||||||
Function / homology | ![]() response to UV-C / DNA synthesis involved in DNA repair / error-free translesion synthesis / cellular response to UV-C / pyrimidine dimer repair / error-prone translesion synthesis / regulation of DNA repair / replication fork / Termination of translesion DNA synthesis / Translesion Synthesis by POLH ...response to UV-C / DNA synthesis involved in DNA repair / error-free translesion synthesis / cellular response to UV-C / pyrimidine dimer repair / error-prone translesion synthesis / regulation of DNA repair / replication fork / Termination of translesion DNA synthesis / Translesion Synthesis by POLH / response to radiation / HDR through Homologous Recombination (HRR) / site of double-strand break / DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gao, Y. / Yang, W. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Capture of a third Mg2+ is essential for catalyzing DNA synthesis. Authors: Gao, Y. / Yang, W. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 128.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 94.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 785 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 791.5 KB | Display | |
Data in XML | ![]() | 23.5 KB | Display | |
Data in CIF | ![]() | 35.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5kfaC ![]() 5kfbC ![]() 5kfcC ![]() 5kfdC ![]() 5kfeC ![]() 5kffC ![]() 5kfgC ![]() 5kfhC ![]() 5kfiC ![]() 5kfjC ![]() 5kfkC ![]() 5kflC ![]() 5kfmC ![]() 5kfnC ![]() 5kfoC ![]() 5kfpC ![]() 5kfqC ![]() 5kfrC ![]() 5kftC ![]() 5kfuC ![]() 5kfvC ![]() 5kfwC ![]() 5kfxC ![]() 5kfyC ![]() 5kfzC ![]() 5kg0C ![]() 5kg1C ![]() 5kg2C ![]() 5kg3C ![]() 5kg4C ![]() 5kg5C ![]() 5kg6C ![]() 5kg7C ![]() 5l9xC ![]() 4ecqS S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-
Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 48531.590 Da / Num. of mol.: 1 / Mutation: R61A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
---|
-DNA chain , 2 types, 2 molecules TP
#2: DNA chain | Mass: 3637.391 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
---|---|
#3: DNA chain | Mass: 2426.617 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
-Non-polymers , 5 types, 453 molecules ![](data/chem/img/CA.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/DTP.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/DTP.gif)
![](data/chem/img/K.gif)
![](data/chem/img/HOH.gif)
#4: Chemical | ChemComp-CA / | ||||||
---|---|---|---|---|---|---|---|
#5: Chemical | #6: Chemical | ChemComp-DTP / | #7: Chemical | ChemComp-K / | #8: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.73 % |
---|---|
Crystal grow | Temperature: 298 K / Method: evaporation / pH: 6 / Details: 17% PEG 2000MME, 0.1 M MES |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jul 3, 2014 / Details: 4*4 mosiac |
Radiation | Monochromator: double crystal-liquid nitrogen cooled / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.46→19.95 Å / Num. obs: 78686 / % possible obs: 100 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 22.21 Å2 / Rmerge(I) obs: 0.065 / Net I/σ(I): 19.76 |
Reflection shell | Resolution: 1.46→1.5 Å / Rmerge(I) obs: 0.941 / Mean I/σ(I) obs: 2.18 / % possible all: 100 |
-
Processing
Software |
| ||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 4ECQ Resolution: 1.46→19.95 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.37
| ||||||||||||||||||
Displacement parameters | Biso mean: 22.81 Å2 | ||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.46→19.95 Å
|