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- PDB-5j3d: Crystal structure of human Fab 14N4 in complex with post-fusion RSV F -
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Open data
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Basic information
Entry | Database: PDB / ID: 5j3d | ||||||
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Title | Crystal structure of human Fab 14N4 in complex with post-fusion RSV F | ||||||
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![]() | IMMUNE SYSTEM / antibody / virus / complex | ||||||
Function / homology | ![]() positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane ...positive regulation of syncytium formation by virus / Translation of respiratory syncytial virus mRNAs / RSV-host interactions / Maturation of hRSV A proteins / Assembly and release of respiratory syncytial virus (RSV) virions / host cell Golgi membrane / Respiratory syncytial virus (RSV) attachment and entry / entry receptor-mediated virion attachment to host cell / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mousa, J.J. / Crowe, J.E. | ||||||
![]() | ![]() Title: Structural basis for nonneutralizing antibody competition at antigenic site II of the respiratory syncytial virus fusion protein. Authors: Mousa, J.J. / Sauer, M.F. / Sevy, A.M. / Finn, J.A. / Bates, J.T. / Alvarado, G. / King, H.G. / Loerinc, L.B. / Fong, R.H. / Doranz, B.J. / Correia, B.E. / Kalyuzhniy, O. / Wen, X. / ...Authors: Mousa, J.J. / Sauer, M.F. / Sevy, A.M. / Finn, J.A. / Bates, J.T. / Alvarado, G. / King, H.G. / Loerinc, L.B. / Fong, R.H. / Doranz, B.J. / Correia, B.E. / Kalyuzhniy, O. / Wen, X. / Jardetzky, T.S. / Schief, W.R. / Ohi, M.D. / Meiler, J. / Crowe, J.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 500.7 KB | Display | ![]() |
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PDB format | ![]() | 409.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 528.9 KB | Display | ![]() |
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Full document | ![]() | 593.8 KB | Display | |
Data in XML | ![]() | 91.8 KB | Display | |
Data in CIF | ![]() | 123.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5itbC ![]() 3rrrS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 23597.490 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Antibody | Mass: 24001.520 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #3: Protein | Mass: 8301.455 Da / Num. of mol.: 3 / Fragment: UNP residues 26-98 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Protein | Mass: 43782.762 Da / Num. of mol.: 3 / Fragment: UNP residues 147-513 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 5.17 Å3/Da / Density % sol: 76.23 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 2 M ammonium sulfate, 5% 2-propanol |
-Data collection
Diffraction | Mean temperature: 80 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX-225 / Detector: CCD / Date: Dec 11, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97872 Å / Relative weight: 1 |
Reflection | Resolution: 4.077→49.5 Å / Num. obs: 91490 / % possible obs: 98.1 % / Redundancy: 8.3 % / CC1/2: 0.987 / Rmerge(I) obs: 0.296 / Net I/σ(I): 5.5 |
Reflection shell | Resolution: 4.077→4.25 Å / Redundancy: 8.5 % / Rmerge(I) obs: 1.191 / Mean I/σ(I) obs: 1.7 / CC1/2: 0.511 / % possible all: 98.7 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3RRR Resolution: 4.077→49.5 Å / SU ML: 0.54 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 26.91 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.077→49.5 Å
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Refine LS restraints |
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LS refinement shell |
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