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- PDB-5ify: Crystal structure of Glucose-1-phosphate thymidylyltransferase fr... -

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Basic information

Entry
Database: PDB / ID: 5ify
TitleCrystal structure of Glucose-1-phosphate thymidylyltransferase from Burkholderia vietnamiensis in complex with 2 -Deoxyuridine-5'-monophosphate and 2'-Deoxy-Thymidine-B-L-Rhamnose
ComponentsGlucose-1-phosphate thymidylyltransferase
KeywordsTRANSFERASE / SSGCID / Glucose-1-phosphate thymidylyltransferase / 2'-Deoxy-Thymidine-B-L-Rhamnose / 2 -Deoxyuridine-5'-monophosphate / Structural Genomics / Seattle Structural Genomics Center for Infectious Disease
Function / homology
Function and homology information


: / glucose-1-phosphate thymidylyltransferase / glucose-1-phosphate thymidylyltransferase activity / metal ion binding
Similarity search - Function
Glucose-1-phosphate thymidylyltransferase, short form / Nucleotidyl transferase domain / Nucleotidyl transferase / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A / Nucleotide-diphospho-sugar transferases / Alpha-Beta Complex / Alpha Beta
Similarity search - Domain/homology
2'-DEOXY-THYMIDINE-BETA-L-RHAMNOSE / 2'-DEOXYURIDINE 5'-MONOPHOSPHATE / Glucose-1-phosphate thymidylyltransferase
Similarity search - Component
Biological speciesBurkholderia vietnamiensis (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.25 Å
AuthorsSeattle Structural Genomics Center for Infectious Disease (SSGCID)
CitationJournal: to be published
Title: Crystal structure of Glucose-1-phosphate thymidylyltransferase from Burkholderia vietnamiensis in complex with 2'-Deoxyuridine-5'-monophosphate and 2'-Deoxy-Thymidine-B-L-Rhamnose
Authors: Abendroth, J. / Dranow, D.M. / Lorimer, D. / Edwards, T.E.
History
DepositionFeb 26, 2016Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 9, 2016Provider: repository / Type: Initial release
Revision 1.1Sep 27, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_prerelease_seq / pdbx_struct_oper_list / struct_ncs_dom_lim
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucose-1-phosphate thymidylyltransferase
B: Glucose-1-phosphate thymidylyltransferase
C: Glucose-1-phosphate thymidylyltransferase
D: Glucose-1-phosphate thymidylyltransferase
E: Glucose-1-phosphate thymidylyltransferase
F: Glucose-1-phosphate thymidylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)209,53918
Polymers204,4006
Non-polymers5,13912
Water15,079837
1
A: Glucose-1-phosphate thymidylyltransferase
B: Glucose-1-phosphate thymidylyltransferase
C: Glucose-1-phosphate thymidylyltransferase
D: Glucose-1-phosphate thymidylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,69312
Polymers136,2674
Non-polymers3,4268
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area18610 Å2
ΔGint-78 kcal/mol
Surface area40170 Å2
MethodPISA
2
E: Glucose-1-phosphate thymidylyltransferase
F: Glucose-1-phosphate thymidylyltransferase
hetero molecules

E: Glucose-1-phosphate thymidylyltransferase
F: Glucose-1-phosphate thymidylyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)139,69312
Polymers136,2674
Non-polymers3,4268
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-x,-y,z1
Buried area19950 Å2
ΔGint-70 kcal/mol
Surface area39630 Å2
MethodPISA
Unit cell
Length a, b, c (Å)109.600, 254.720, 82.050
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP21212
Components on special symmetry positions
IDModelComponents
11E-691-

HOH

21F-647-

HOH

31F-701-

HOH

Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and (resid 2:57 or (resid 58 and (name...
21(chain B and (resid 2:57 or (resid 58 and (name...
31(chain C and (resid 2:57 or (resid 58 and (name...
41(chain D and (resid 2:65 or resid 67:100 or resid...
51(chain E and (resid 2:57 or (resid 58 and (name...
61(chain F and (resid 2:57 or (resid 58 and (name...

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ALAALAPROPRO(chain A and (resid 2:57 or (resid 58 and (name...AA2 - 5710 - 65
12GLNGLNGLNGLN(chain A and (resid 2:57 or (resid 58 and (name...AA5866
13ALAALAUMPUMP(chain A and (resid 2:57 or (resid 58 and (name...AA - H2 - 50110
14ALAALAUMPUMP(chain A and (resid 2:57 or (resid 58 and (name...AA - H2 - 50110
15ALAALAUMPUMP(chain A and (resid 2:57 or (resid 58 and (name...AA - H2 - 50110
16ALAALAUMPUMP(chain A and (resid 2:57 or (resid 58 and (name...AA - H2 - 50110
21ALAALAPROPRO(chain B and (resid 2:57 or (resid 58 and (name...BB2 - 5710 - 65
22GLNGLNGLNGLN(chain B and (resid 2:57 or (resid 58 and (name...BB5866
23METMETUMPUMP(chain B and (resid 2:57 or (resid 58 and (name...BB - J1 - 5019
24METMETUMPUMP(chain B and (resid 2:57 or (resid 58 and (name...BB - J1 - 5019
25METMETUMPUMP(chain B and (resid 2:57 or (resid 58 and (name...BB - J1 - 5019
26METMETUMPUMP(chain B and (resid 2:57 or (resid 58 and (name...BB - J1 - 5019
31ALAALAPROPRO(chain C and (resid 2:57 or (resid 58 and (name...CC2 - 5710 - 65
32GLNGLNGLNGLN(chain C and (resid 2:57 or (resid 58 and (name...CC5866
33ALAALAUMPUMP(chain C and (resid 2:57 or (resid 58 and (name...CC - L2 - 50110
34ALAALAUMPUMP(chain C and (resid 2:57 or (resid 58 and (name...CC - L2 - 50110
35ALAALAUMPUMP(chain C and (resid 2:57 or (resid 58 and (name...CC - L2 - 50110
36ALAALAUMPUMP(chain C and (resid 2:57 or (resid 58 and (name...CC - L2 - 50110
41ALAALASERSER(chain D and (resid 2:65 or resid 67:100 or resid...DD2 - 6510 - 73
42LEULEUGLYGLY(chain D and (resid 2:65 or resid 67:100 or resid...DD67 - 10075 - 108
43GLUGLUGLUGLU(chain D and (resid 2:65 or resid 67:100 or resid...DD102 - 123110 - 131
44ARGARGALAALA(chain D and (resid 2:65 or resid 67:100 or resid...DD124 - 125132 - 133
45ALAALAUMPUMP(chain D and (resid 2:65 or resid 67:100 or resid...DD - N2 - 50110
46ALAALAUMPUMP(chain D and (resid 2:65 or resid 67:100 or resid...DD - N2 - 50110
47ALAALAUMPUMP(chain D and (resid 2:65 or resid 67:100 or resid...DD - N2 - 50110
48ALAALAUMPUMP(chain D and (resid 2:65 or resid 67:100 or resid...DD - N2 - 50110
51ALAALAPROPRO(chain E and (resid 2:57 or (resid 58 and (name...EE2 - 5710 - 65
52GLNGLNGLNGLN(chain E and (resid 2:57 or (resid 58 and (name...EE5866
53ALAALAUMPUMP(chain E and (resid 2:57 or (resid 58 and (name...EE - P2 - 50110
54ALAALAUMPUMP(chain E and (resid 2:57 or (resid 58 and (name...EE - P2 - 50110
55ALAALAUMPUMP(chain E and (resid 2:57 or (resid 58 and (name...EE - P2 - 50110
56ALAALAUMPUMP(chain E and (resid 2:57 or (resid 58 and (name...EE - P2 - 50110
61ALAALAPROPRO(chain F and (resid 2:57 or (resid 58 and (name...FF2 - 5710 - 65
62GLNGLNGLNGLN(chain F and (resid 2:57 or (resid 58 and (name...FF5866
63ALAALAUMPUMP(chain F and (resid 2:57 or (resid 58 and (name...FF - R2 - 50110
64ALAALAUMPUMP(chain F and (resid 2:57 or (resid 58 and (name...FF - R2 - 50110
65ALAALAUMPUMP(chain F and (resid 2:57 or (resid 58 and (name...FF - R2 - 50110
66ALAALAUMPUMP(chain F and (resid 2:57 or (resid 58 and (name...FF - R2 - 50110

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Components

#1: Protein
Glucose-1-phosphate thymidylyltransferase


Mass: 34066.629 Da / Num. of mol.: 6 / Fragment: BuviA.00118.a.B1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Burkholderia vietnamiensis (strain G4 / LMG 22486) (bacteria)
Strain: G4 / LMG 22486 / Gene: Bcep1808_0806 / Plasmid: BuviA.00118.a.B1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)
References: UniProt: A4JC15, glucose-1-phosphate thymidylyltransferase
#2: Chemical
ChemComp-TRH / 2'-DEOXY-THYMIDINE-BETA-L-RHAMNOSE


Mass: 548.330 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C16H26N2O15P2
#3: Chemical
ChemComp-UMP / 2'-DEOXYURIDINE 5'-MONOPHOSPHATE / DUMP


Mass: 308.182 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C9H13N2O8P
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 837 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.8 Å3/Da / Density % sol: 56 %
Crystal growTemperature: 290 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: MCSG1 b8: 25.5% PEG 4000, 15% Glycerol, 170mM NaOAc, 100mM Tris base/HCl pH 8.5 BuviA.00118.a.B1.PS02510 (21mg/ml) + 4mM each Glucose-1-phosphate, dUTP (neither of which is bound); cryo: ...Details: MCSG1 b8: 25.5% PEG 4000, 15% Glycerol, 170mM NaOAc, 100mM Tris base/HCl pH 8.5 BuviA.00118.a.B1.PS02510 (21mg/ml) + 4mM each Glucose-1-phosphate, dUTP (neither of which is bound); cryo: direct; tray 267382b8, puck zto7-1

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.97856 Å
DetectorType: RAYONIX MX-300 / Detector: CCD / Date: Dec 19, 2015
RadiationMonochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97856 Å / Relative weight: 1
ReflectionResolution: 2.25→46.197 Å / Num. obs: 109242 / % possible obs: 99.5 % / Observed criterion σ(I): -3 / Biso Wilson estimate: 33.85 Å2 / CC1/2: 0.997 / Rmerge(I) obs: 0.075 / Net I/σ(I): 14.52
Reflection shell
Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsDiffraction-ID% possible all
2.25-2.3150.4843.07199.9
2.31-2.370.4253.54199.9
2.37-2.440.3773.95199.9
2.44-2.520.2955.02199.9
2.52-2.60.2416.16199.8
2.6-2.690.2077.11199.8
2.69-2.790.1738.46199.9
2.79-2.90.13710.51199.8
2.9-3.030.1112.8199.8
3.03-3.180.08915.45199.8
3.18-3.350.07418.39199.7
3.35-3.560.06122.09199.5
3.56-3.80.05225.13199.4
3.8-4.110.04528.81199.3
4.11-4.50.04131.38199.2
4.5-5.030.03832.78198.9
5.03-5.810.03831.92198.8
5.81-7.120.0431.43198.5
7.12-10.060.03635.37197.8
10.06-46.1970.03533.81192.3

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
XSCALEdata scaling
MOLREPphasing
PHENIXdev_2328refinement
PDB_EXTRACTdata extraction
XDSdata reduction
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: pdb entry 1fxo, chain A

1fxo


Resolution: 2.25→46.197 Å / SU ML: 0.23 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 20.04
RfactorNum. reflection% reflection
Rfree0.197 1984 1.82 %
Rwork0.1569 --
obs0.1576 109167 99.46 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 121.78 Å2 / Biso mean: 42.2469 Å2 / Biso min: 13.21 Å2
Refinement stepCycle: final / Resolution: 2.25→46.197 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13303 0 330 837 14470
Biso mean--37.87 40.98 -
Num. residues----1753
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00913996
X-RAY DIFFRACTIONf_angle_d0.99519143
X-RAY DIFFRACTIONf_chiral_restr0.0592151
X-RAY DIFFRACTIONf_plane_restr0.0072554
X-RAY DIFFRACTIONf_dihedral_angle_d13.8698346
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A7307X-RAY DIFFRACTION8.595TORSIONAL
12B7307X-RAY DIFFRACTION8.595TORSIONAL
13C7307X-RAY DIFFRACTION8.595TORSIONAL
14D7307X-RAY DIFFRACTION8.595TORSIONAL
15E7307X-RAY DIFFRACTION8.595TORSIONAL
16F7307X-RAY DIFFRACTION8.595TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.25-2.30630.28271280.211276047732100
2.3063-2.36870.26351390.201676407779100
2.3687-2.43840.22611260.190276017727100
2.4384-2.51710.23391580.177475647722100
2.5171-2.6070.21491290.170276207749100
2.607-2.71140.22991480.1875927740100
2.7114-2.83480.21841680.175276587826100
2.8348-2.98420.25371320.170676267758100
2.9842-3.17110.21671340.173876577791100
3.1711-3.41590.18451500.167376697819100
3.4159-3.75950.2081350.149776617796100
3.7595-4.30320.17491410.12627699784099
4.3032-5.42020.13551450.12447745789099
5.4202-46.2070.18011510.15497847799897
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.3845-0.6919-0.99721.18090.34842.4618-0.2842-0.5366-0.39270.14830.16660.03190.38130.20490.08450.21210.06850.07740.29330.11250.2997-32.405243.8033-18.9121
22.7567-0.38830.10062.91070.18050.9016-0.01690.0438-0.4437-0.1867-0.05990.11030.1746-0.09010.07330.23360.01490.05440.18030.01290.256-31.526643.6211-31.61
34.2946-0.03371.72480.2150.91195.545-0.0954-0.17230.9738-0.8218-0.2194-0.547-0.91050.24430.07010.53780.08790.12270.15930.12650.459-16.669361.5913-28.8248
42.9283-0.31830.59782.05421.43473.0308-0.01240.0123-0.0178-0.17780.0912-0.4730.10450.1561-0.04040.18590.01540.10170.15660.04580.3072-15.556449.2112-31.2216
50.59780.0504-0.28312.73832.52472.5227-0.05570.0785-0.1529-0.344-0.0423-0.1054-0.62530.3914-0.05760.3147-0.02480.08340.16750.01180.2706-15.464151.3933-37.9392
62.11080.3763-0.7692.19990.10461.5027-0.0591-0.0774-0.2697-0.1242-0.01820.16480.1021-0.0860.10830.16670.04040.01020.18560.05690.2725-42.93752.5609-26.6072
72.7381-1.04982.61582.6835-4.38149.0839-0.1742-0.26040.02070.00710.20020.8068-0.3888-1.2145-0.08860.26310.0086-0.00240.3469-0.00420.5276-55.106451.1079-29.3986
81.4179-0.38651.03432.5583-1.52174.6838-0.0344-0.16310.03260.18120.17370.3309-0.4737-0.332-0.08440.19130.0498-0.02310.17140.00290.2204-44.938281.8534-16.8332
92.1878-0.75220.32212.3691-0.10921.0869-0.04040.03690.2299-0.0455-0.0365-0.1449-0.23920.00460.07020.21880.0242-0.03810.16670.0270.1512-42.686985.0956-28.5711
102.2249-0.0753-0.42713.8778-0.78182.1638-0.04550.0226-0.1-0.17710.03290.46430.0433-0.14940.01620.15890.0425-0.07460.2385-0.01050.1884-57.815675.9268-33.4545
112.0664-0.08231.02950.85870.14711.0746-0.02290.0526-0.0179-0.07270.01090.0427-0.02180.01970.02290.17470.0290.01240.1824-0.00410.1459-40.615274.0872-27.7154
122.53140.2301-0.74575.5411-2.5842.27510.0037-0.04820.1018-0.145-0.0982-0.09170.03310.47470.15770.21380.0127-0.06170.28870.04320.3102-19.648280.9007-20.0327
130.54080.3485-1.19910.6007-1.47764.7833-0.1639-0.4115-0.38240.04160.05590.08840.71240.23210.06340.36140.15620.10270.46430.18040.3937-42.443240.7092-1.4341
142.2822-0.2333-0.34291.6484-0.8481.3943-0.0643-0.1953-0.6741-0.3371-0.3096-0.22771.00040.50210.28730.67670.2660.20920.49180.16790.4477-44.466434.64763.1517
153.9590.3557-0.28852.6152-0.81842.45830.0048-0.09780.16220.2973-0.11470.0279-0.08430.26030.00650.30930.03270.08480.41010.09230.2803-56.076450.718511.4257
161.64950.4256-1.211.4737-0.60512.3118-0.1394-0.3452-0.33440.0915-0.0609-0.1240.3760.45980.07450.28020.13550.04510.48620.12220.2604-45.444745.87585.6489
170.6092-0.6808-1.43710.97930.87926.86260.319-0.77020.59960.4464-0.050.0591-0.74280.6375-0.2420.341-0.00870.02010.404-0.05880.2773-37.355780.5641-0.8215
181.4165-0.49190.04973.20710.52882.43060.0614-0.80540.3460.6642-0.0425-0.1717-0.65890.1093-0.03110.5466-0.06050.02860.7212-0.12860.2866-40.01980.491811.6703
191.95010.2084-0.18914.48871.92972.72180.0558-1.04690.20480.84570.1512-1.1826-0.15840.831-0.24540.5932-0.1173-0.23581.2536-0.08290.5295-24.739673.326516.9855
200.58740.25910.29452.544-0.31592.30620.0143-0.6582-0.00550.2694-0.01950.3698-0.1052-0.1349-0.00850.23450.00420.02050.48810.01530.2179-48.482970.53633.5062
212.19090.1823-1.72592.2444-0.7897.48290.0103-0.2254-0.19830.13110.2441.13560.5054-0.9602-0.50850.33610.03340.05880.63380.04420.5429-61.236270.20273.1268
221.6212-0.4981-0.62971.81770.99212.27790.06180.04210.14670.04790.19340.1735-0.2468-0.1463-0.16850.27860.00380.02950.20770.06780.3625-13.51314.6728-32.9274
232.6179-0.613-0.13972.64310.64511.5053-0.1138-0.14280.09270.3720.1910.5327-0.016-0.214-0.09630.32990.04950.0920.21290.07280.3681-18.211615.2858-22.5584
244.63811.3969-2.37066.6035-1.46363.4594-0.3046-0.5649-0.06420.46840.1919-0.43540.33460.43150.06940.41540.139-0.07090.2385-0.02320.31610.863715.0404-15.2115
251.3257-0.42090.08322.40330.44721.0615-0.0778-0.15070.06380.43280.23550.10850.0186-0.061-0.14410.33690.05750.01960.18790.02990.2635-7.701112.946-20.5863
263.2818-2.91670.95767.7615-5.52764.4994-0.1342-0.246-0.76710.0521-0.09370.41981.2926-0.12780.20720.5458-0.02440.06350.32480.12310.7996-19.9074-9.6743-25.5765
270.34990.23630.08431.7473-0.36928.7343-0.01850.0464-0.0769-0.10230.22150.6165-0.2592-0.9486-0.10390.2981-0.094-0.12670.36120.14940.6679-22.42435.2689-47.9208
281.37270.5583-0.11891.81260.08771.20010.06520.0016-0.153-0.19680.23720.629-0.3924-0.5782-0.2130.31190.0028-0.05930.32980.12440.4226-17.558312.2911-52.3894
290.6592-0.1953-0.00932.1748-0.09151.0575-0.01770.1544-0.2588-0.52530.25730.8952-0.0438-0.4868-0.16350.3988-0.0945-0.21640.45090.17040.6253-22.6639.0894-59.8332
304.55050.54180.73850.2443-0.37454.45-0.14790.4394-0.2542-0.83910.16330.47160.4530.0025-0.0650.631-0.1254-0.1740.314-0.00540.37-13.6043-6.9725-64.9105
310.538-0.6621-0.32892.7787-0.13611.6556-0.03720.1899-0.2688-0.63570.2720.88080.3779-0.4413-0.08560.4992-0.2398-0.27350.34070.10640.5261-17.7653-4.3645-59.6175
321.90030.19971.14155.8783-0.22613.5398-0.00870.2091-0.0075-0.58140.1142-0.1224-0.07030.1483-0.25690.3094-0.06440.0060.19670.02050.2366-3.560422.3685-57.0856
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 2 through 34 )A2 - 34
2X-RAY DIFFRACTION2chain 'A' and (resid 35 through 138 )A35 - 138
3X-RAY DIFFRACTION3chain 'A' and (resid 139 through 155 )A139 - 155
4X-RAY DIFFRACTION4chain 'A' and (resid 156 through 198 )A156 - 198
5X-RAY DIFFRACTION5chain 'A' and (resid 199 through 216 )A199 - 216
6X-RAY DIFFRACTION6chain 'A' and (resid 217 through 275 )A217 - 275
7X-RAY DIFFRACTION7chain 'A' and (resid 276 through 294 )A276 - 294
8X-RAY DIFFRACTION8chain 'B' and (resid 1 through 34 )B1 - 34
9X-RAY DIFFRACTION9chain 'B' and (resid 35 through 138 )B35 - 138
10X-RAY DIFFRACTION10chain 'B' and (resid 139 through 198 )B139 - 198
11X-RAY DIFFRACTION11chain 'B' and (resid 199 through 264 )B199 - 264
12X-RAY DIFFRACTION12chain 'B' and (resid 265 through 293 )B265 - 293
13X-RAY DIFFRACTION13chain 'C' and (resid 2 through 55 )C2 - 55
14X-RAY DIFFRACTION14chain 'C' and (resid 56 through 117 )C56 - 117
15X-RAY DIFFRACTION15chain 'C' and (resid 118 through 155 )C118 - 155
16X-RAY DIFFRACTION16chain 'C' and (resid 156 through 290 )C156 - 290
17X-RAY DIFFRACTION17chain 'D' and (resid 2 through 34 )D2 - 34
18X-RAY DIFFRACTION18chain 'D' and (resid 35 through 138 )D35 - 138
19X-RAY DIFFRACTION19chain 'D' and (resid 139 through 217 )D139 - 217
20X-RAY DIFFRACTION20chain 'D' and (resid 218 through 275 )D218 - 275
21X-RAY DIFFRACTION21chain 'D' and (resid 276 through 294 )D276 - 294
22X-RAY DIFFRACTION22chain 'E' and (resid 2 through 34 )E2 - 34
23X-RAY DIFFRACTION23chain 'E' and (resid 35 through 117 )E35 - 117
24X-RAY DIFFRACTION24chain 'E' and (resid 118 through 155 )E118 - 155
25X-RAY DIFFRACTION25chain 'E' and (resid 156 through 275 )E156 - 275
26X-RAY DIFFRACTION26chain 'E' and (resid 276 through 294 )E276 - 294
27X-RAY DIFFRACTION27chain 'F' and (resid 2 through 24 )F2 - 24
28X-RAY DIFFRACTION28chain 'F' and (resid 25 through 67 )F25 - 67
29X-RAY DIFFRACTION29chain 'F' and (resid 68 through 117 )F68 - 117
30X-RAY DIFFRACTION30chain 'F' and (resid 118 through 155 )F118 - 155
31X-RAY DIFFRACTION31chain 'F' and (resid 156 through 246 )F156 - 246
32X-RAY DIFFRACTION32chain 'F' and (resid 247 through 293 )F247 - 293

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