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Yorodumi- PDB-5i0k: Insights into Substrate Modification by Dehydratases from Type I ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5i0k | |||||||||
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Title | Insights into Substrate Modification by Dehydratases from Type I Polyketide Synthases | |||||||||
Components | Phthiocerol synthesis polyketide synthase type I PpsC | |||||||||
Keywords | TRANSFERASE / dehydratase / polyketide / complex / tuberculosis | |||||||||
Function / homology | Function and homology information (phenol)carboxyphthiodiolenone synthase / phthiocerol biosynthetic process / phenolic phthiocerol biosynthetic process / polyketide synthase complex / Actinobacterium-type cell wall biogenesis / DIM/DIP cell wall layer assembly / fatty acid synthase activity / secondary metabolite biosynthetic process / phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity ...(phenol)carboxyphthiodiolenone synthase / phthiocerol biosynthetic process / phenolic phthiocerol biosynthetic process / polyketide synthase complex / Actinobacterium-type cell wall biogenesis / DIM/DIP cell wall layer assembly / fatty acid synthase activity / secondary metabolite biosynthetic process / phosphopantetheine binding / 3-oxoacyl-[acyl-carrier-protein] synthase activity / fatty acid biosynthetic process / oxidoreductase activity / plasma membrane / cytoplasm / cytosol Similarity search - Function | |||||||||
Biological species | Mycobacterium tuberculosis (bacteria) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.197 Å | |||||||||
Authors | Faille, A. / Mourey, L. / Pedelacq, J.D. | |||||||||
Citation | Journal: J. Mol. Biol. / Year: 2017 Title: Insights into Substrate Modification by Dehydratases from Type I Polyketide Synthases. Authors: Faille, A. / Gavalda, S. / Slama, N. / Lherbet, C. / Maveyraud, L. / Guillet, V. / Laval, F. / Quemard, A. / Mourey, L. / Pedelacq, J.D. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5i0k.cif.gz | 66.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5i0k.ent.gz | 47.5 KB | Display | PDB format |
PDBx/mmJSON format | 5i0k.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5i0k_validation.pdf.gz | 686.8 KB | Display | wwPDB validaton report |
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Full document | 5i0k_full_validation.pdf.gz | 696.2 KB | Display | |
Data in XML | 5i0k_validation.xml.gz | 13.4 KB | Display | |
Data in CIF | 5i0k_validation.cif.gz | 16.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/i0/5i0k ftp://data.pdbj.org/pub/pdb/validation_reports/i0/5i0k | HTTPS FTP |
-Related structure data
Related structure data | 5l84C 5njiC 4oocS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 34311.336 Da / Num. of mol.: 1 / Fragment: UNP residues 921-1224 / Mutation: H959F Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mycobacterium tuberculosis (bacteria) / Gene: ppsC, Rv2933 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: P96202, beta-ketoacyl-[acyl-carrier-protein] synthase I |
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#2: Chemical | ChemComp-COO / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.26 Å3/Da / Density % sol: 71.1 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6 / Details: Na/K PO4 1.8 M pH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID29 / Wavelength: 0.97625 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 25, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97625 Å / Relative weight: 1 |
Reflection | Resolution: 3.1→50 Å / Num. obs: 10715 / % possible obs: 95.3 % / Redundancy: 4.76 % / Net I/σ(I): 7.83 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4OOC Resolution: 3.197→46.39 Å / SU ML: 0.45 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 34.89 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.197→46.39 Å
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Refine LS restraints |
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LS refinement shell |
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