#48 - Dec 2003 Catabolite Activator Protein similarity (8)
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Assembly
Deposited unit
A: cAMP-dependent protein kinase type I-alpha regulatory subunit B: cAMP-dependent protein kinase type I-alpha regulatory subunit C: Small membrane A-kinase anchor protein
Mass: 18.015 Da / Num. of mol.: 29 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.02 Å3/Da / Density % sol: 39.17 %
Crystal grow
Temperature: 297 K / Method: microbatch / pH: 3.5 Details: a 2:3 ratio of protein solution:crystallizing (crystallizing solution: 0.1 M Citric acid pH 3.5, 28% w/v Polyethylene glycol 8,000) Temp details: room temperature
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Data collection
Diffraction
Mean temperature: 200 K / Ambient temp details: cryo under liquid nitrogen
Diffraction source
Source: SYNCHROTRON / Site: ALS / Beamline: 8.2.2 / Wavelength: 1 Å
Resolution: 2→39.94 Å / Cor.coef. Fo:Fc: 0.939 / Cor.coef. Fo:Fc free: 0.917 / SU B: 3.823 / SU ML: 0.111 / Cross valid method: THROUGHOUT / ESU R: 0.214 / ESU R Free: 0.175 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.24133
392
4.7 %
RANDOM
Rwork
0.20976
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obs
0.21127
7937
97.53 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å