+Open data
-Basic information
Entry | Database: PDB / ID: 5hc5 | ||||||
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Title | The structure of esterase Est22 mutant-S188A | ||||||
Components | Lipolytic enzyme | ||||||
Keywords | HYDROLASE / esterase / Est22 | ||||||
Function / homology | Function and homology information Hydrolases; Acting on ester bonds; Carboxylic-ester hydrolases / hydrolase activity Similarity search - Function | ||||||
Biological species | uncultured bacterium (environmental samples) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 2.43 Å | ||||||
Authors | Li, J. / Huang, J. | ||||||
Citation | Journal: Sci Rep / Year: 2016 Title: Structural insights of a hormone sensitive lipase homologue Est22. Authors: Huang, J. / Huo, Y.Y. / Ji, R. / Kuang, S. / Ji, C. / Xu, X.W. / Li, J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5hc5.cif.gz | 262.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5hc5.ent.gz | 214.1 KB | Display | PDB format |
PDBx/mmJSON format | 5hc5.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hc/5hc5 ftp://data.pdbj.org/pub/pdb/validation_reports/hc/5hc5 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 39066.684 Da / Num. of mol.: 4 / Mutation: S188A Source method: isolated from a genetically manipulated source Source: (gene. exp.) uncultured bacterium (environmental samples) Production host: Escherichia coli (E. coli) References: UniProt: H6BDX1, Hydrolases; Acting on ester bonds; Carboxylic-ester hydrolases #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.53 Å3/Da / Density % sol: 51.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: 1M Sodium citrate, 0.1M imidazole, pH 8.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL18U1 / Wavelength: 0.9785 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 6, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 2.43→94.56 Å / Num. obs: 55338 / % possible obs: 96.2 % / Redundancy: 5.4 % / Net I/σ(I): 8.66 |
-Processing
Software |
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Refinement | Resolution: 2.43→50 Å / Cor.coef. Fo:Fc: 0.954 / Cor.coef. Fo:Fc free: 0.915 / SU B: 10.021 / SU ML: 0.212 / Cross valid method: THROUGHOUT / ESU R: 0.587 / ESU R Free: 0.275 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.011 Å2
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Refinement step | Cycle: LAST / Resolution: 2.43→50 Å
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Refine LS restraints |
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