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- PDB-5fzp: Structure of the dispase autolysis inducing protein from Streptom... -
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Open data
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Basic information
Entry | Database: PDB / ID: 5fzp | ||||||
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Title | Structure of the dispase autolysis inducing protein from Streptomyces mobaraensis | ||||||
![]() | DISPASE AUTOLYSIS-INDUCING PROTEIN | ||||||
![]() | SIGNALING PROTEIN / DISPASE-AUTOLYSIS INDUCING PROTEIN / DAIP / GLUTAMINE CROSS- LINKING SITES / STREPTOMYCES MOBARAENSIS / 7-BLADED BETA-PROPELLER | ||||||
Function / homology | WD40/YVTN repeat-like-containing domain superfamily / extracellular region / Dispase autolysis-inducing protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Schmelz, S. / Fiebig, D. / Beck, J. / Fuchsbauer, H.L. / Scrima, A. | ||||||
![]() | ![]() Title: Structure of the Dispase Autolysis Inducing Protein from Streptomyces Mobaraensis and Glutamine Cross-Linking Sites for Transglutaminase Authors: Fiebig, D. / Schmelz, S. / Zindel, S. / Ehret, V. / Beck, J. / Ebenig, A. / Ehret, M. / Froels, S. / Pfeifer, F. / Kolmar, H. / Fuchsbauer, H.L. / Scrima, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 152.3 KB | Display | ![]() |
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PDB format | ![]() | 128.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 448.8 KB | Display | ![]() |
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Full document | ![]() | 452.3 KB | Display | |
Data in XML | ![]() | 33.2 KB | Display | |
Data in CIF | ![]() | 51 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 36187.570 Da / Num. of mol.: 2 / Fragment: UNP RESIDUES 27-374 / Source method: isolated from a natural source Details: GERMAN COLLECTION OF MICROORGANISMS AND CELL CULTURES, BRAUNSCHWEIG, GERMANY Source: (natural) ![]() #2: Chemical | ChemComp-GOL / #3: Chemical | ChemComp-CA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 54.4 % / Description: NONE |
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Crystal grow | Details: 15% (V/V) GLYCEROL, 8.5% (V/V) 2-PROPANOL, 85 MM HEPES PH 7.5 AND 17% (W/V) PEG 4K WITH 10 MG/ML DAIP PROTEIN (IN WATER) |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Date: Jun 13, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.38568 Å / Relative weight: 1 |
Reflection | Resolution: 1.7→42.3 Å / Num. obs: 148865 / % possible obs: 98.7 % / Observed criterion σ(I): 3 / Redundancy: 2.7 % / Biso Wilson estimate: 16.71 Å2 / Rmerge(I) obs: 0.04 / Net I/σ(I): 15.56 |
Reflection shell | Resolution: 1.7→1.8 Å / Redundancy: 2.6 % / Rmerge(I) obs: 0.32 / Mean I/σ(I) obs: 3.02 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 1.7→19.938 Å / SU ML: 0.15 / σ(F): 1.25 / Phase error: 18.75 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.7→19.938 Å
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Refine LS restraints |
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LS refinement shell |
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