- PDB-5fkq: Unraveling the first step of xyloglucan degradation by the soil s... -
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Basic information
Entry
Database: PDB / ID: 5fkq
Title
Unraveling the first step of xyloglucan degradation by the soil saprophyte Cellvibrio japonicus through the functional and structural characterization of a potent GH74 endo-xyloglucanase
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9795 Å / Relative weight: 1
Reflection
Resolution: 1.71→48.88 Å / Num. obs: 172392 / % possible obs: 99.8 % / Observed criterion σ(I): 2 / Redundancy: 4.1 % / Rmerge(I) obs: 0.1 / Net I/σ(I): 11
Reflection shell
Resolution: 1.71→1.75 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.73 / Mean I/σ(I) obs: 1.6 / % possible all: 99.7
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0131
refinement
Aimless
datascaling
MOLREP
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT Starting model: APO STRUCTURE Resolution: 1.71→102.82 Å / Cor.coef. Fo:Fc: 0.94 / Cor.coef. Fo:Fc free: 0.921 / SU B: 2.678 / SU ML: 0.084 / Cross valid method: THROUGHOUT / ESU R: 0.108 / ESU R Free: 0.106 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES, REFINED INDIVIDUALLY
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22462
8556
5 %
RANDOM
Rwork
0.19248
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obs
0.19408
163901
99.9 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK