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Yorodumi- PDB-5ewe: Ternary complex of human DNA polymerase eta inserting rCTP opposi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 5ewe | |||||||||
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Title | Ternary complex of human DNA polymerase eta inserting rCTP opposite template G | |||||||||
Components |
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Keywords | TRANSFERASE/DNA / Polymerase-DNA Complex ribonucleotide incorporation / TRANSFERASE-DNA complex | |||||||||
Function / homology | Function and homology information response to UV-C / error-free translesion synthesis / DNA synthesis involved in DNA repair / cellular response to UV-C / pyrimidine dimer repair / error-prone translesion synthesis / regulation of DNA repair / replication fork / Termination of translesion DNA synthesis / Translesion Synthesis by POLH ...response to UV-C / error-free translesion synthesis / DNA synthesis involved in DNA repair / cellular response to UV-C / pyrimidine dimer repair / error-prone translesion synthesis / regulation of DNA repair / replication fork / Termination of translesion DNA synthesis / Translesion Synthesis by POLH / response to radiation / HDR through Homologous Recombination (HRR) / site of double-strand break / DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / DNA repair / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) synthetic construct (others) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.66 Å | |||||||||
Authors | Su, Y. / Egli, M. / Guengerich, F.P. | |||||||||
Funding support | United States, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2016 Title: Mechanism of Ribonucleotide Incorporation by Human DNA Polymerase eta. Authors: Su, Y. / Egli, M. / Guengerich, F.P. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5ewe.cif.gz | 128.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5ewe.ent.gz | 92.8 KB | Display | PDB format |
PDBx/mmJSON format | 5ewe.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 5ewe_validation.pdf.gz | 777.4 KB | Display | wwPDB validaton report |
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Full document | 5ewe_full_validation.pdf.gz | 780 KB | Display | |
Data in XML | 5ewe_validation.xml.gz | 22.7 KB | Display | |
Data in CIF | 5ewe_validation.cif.gz | 34.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ew/5ewe ftp://data.pdbj.org/pub/pdb/validation_reports/ew/5ewe | HTTPS FTP |
-Related structure data
Related structure data | 5ewfC 5ewgC 4o3nS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 48617.707 Da / Num. of mol.: 1 / Fragment: UNP residues 1-432 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: POLH, RAD30, RAD30A, XPV Production host: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (bacteria) References: UniProt: Q9Y253, DNA-directed DNA polymerase |
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-DNA chain , 2 types, 2 molecules TP
#2: DNA chain | Mass: 3662.404 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
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#3: DNA chain | Mass: 2426.617 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Non-polymers , 4 types, 438 molecules
#4: Chemical | ChemComp-CTP / | ||||
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#5: Chemical | #6: Chemical | #7: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.25 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 Details: NaMES (pH 6.0), polyethylene glycol monomethyl ether 2000, and calcium chloride |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.97856 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Oct 8, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97856 Å / Relative weight: 1 |
Reflection | Resolution: 1.66→50 Å / Num. obs: 54114 / % possible obs: 100 % / Redundancy: 8.9 % / Rmerge(I) obs: 0.098 / Net I/σ(I): 19.2 |
Reflection shell | Resolution: 1.66→1.69 Å / Redundancy: 8.8 % / Rmerge(I) obs: 0.978 / Mean I/σ(I) obs: 2.4 / % possible all: 100 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4O3N Resolution: 1.66→42.99 Å / SU ML: 0.18 / Cross valid method: THROUGHOUT / σ(F): 0.02 / Phase error: 21.71 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.66→42.99 Å
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Refine LS restraints |
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LS refinement shell |
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