Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature
要素
Thermonuclease
キーワード
HYDROLASE / nuclease / hyperstable / pdTp / ionizable group
機能・相同性
機能・相同性情報
endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / micrococcal nuclease / nucleic acid binding / extracellular region / membrane / metal ion binding / cytoplasm 類似検索 - 分子機能
Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) ...Thermonuclease family signature 1. / OB fold (Dihydrolipoamide Acetyltransferase, E2P) - #90 / Thermonuclease active site / Thermonuclease family signature 2. / Staphylococcal nuclease (SNase-like), OB-fold / Staphylococcal nuclease homologue / Thermonuclease domain profile. / Staphylococcal nuclease homologues / SNase-like, OB-fold superfamily / OB fold (Dihydrolipoamide Acetyltransferase, E2P) / Beta Barrel / Mainly Beta 類似検索 - ドメイン・相同性
解像度: 2.1→47.89 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.894 / WRfactor Rfree: 0.2255 / WRfactor Rwork: 0.1718 / FOM work R set: 0.8571 / SU B: 8.689 / SU ML: 0.127 / SU R Cruickshank DPI: 0.2054 / SU Rfree: 0.1877 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.205 / ESU R Free: 0.188 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2442
450
4.9 %
RANDOM
Rwork
0.1854
-
-
-
obs
0.1883
8689
99.98 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK