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- PDB-5ej4: EcMenD-ThDP-Mn2+ complex soaked with 2-ketoglutarate for 15 min -

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Basic information

Entry
Database: PDB / ID: 5ej4
TitleEcMenD-ThDP-Mn2+ complex soaked with 2-ketoglutarate for 15 min
Components2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
KeywordsTRANSFERASE / Post-decarboxylation intermediate
Function / homology
Function and homology information


2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity / menaquinone biosynthetic process / thiamine pyrophosphate binding / manganese ion binding / magnesium ion binding / protein homodimerization activity
Similarity search - Function
Menaquinone biosynthesis protein MenD, middle domain / Middle domain of thiamine pyrophosphate / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase / Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain / Thiamine pyrophosphate enzyme, N-terminal TPP binding domain / Thiamine pyrophosphate enzyme, C-terminal TPP-binding / Thiamine pyrophosphate enzyme, C-terminal TPP binding domain / Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains / TPP-binding domain / Thiamin diphosphate-binding fold ...Menaquinone biosynthesis protein MenD, middle domain / Middle domain of thiamine pyrophosphate / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase / Thiamine pyrophosphate enzyme, N-terminal TPP-binding domain / Thiamine pyrophosphate enzyme, N-terminal TPP binding domain / Thiamine pyrophosphate enzyme, C-terminal TPP-binding / Thiamine pyrophosphate enzyme, C-terminal TPP binding domain / Thiamin diphosphate (ThDP)-binding fold, Pyr/PP domains / TPP-binding domain / Thiamin diphosphate-binding fold / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
FORMIC ACID / : / Chem-TD6 / 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Similarity search - Component
Biological speciesEscherichia coli K12 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.773 Å
AuthorsSong, H.G. / Dong, C. / Chen, Y.Z. / Sun, Y.R. / Guo, Z.H.
CitationJournal: J.Am.Chem.Soc. / Year: 2016
Title: A Thiamine-Dependent Enzyme Utilizes an Active Tetrahedral Intermediate in Vitamin K Biosynthesis
Authors: Song, H.G. / Dong, C. / Qin, M.M. / Chen, Y.Z. / Sun, Y.R. / Liu, J.J. / Chan, W. / Guo, Z.H.
History
DepositionNov 1, 2015Deposition site: RCSB / Processing site: PDBJ
Revision 1.0Jun 1, 2016Provider: repository / Type: Initial release
Revision 1.1Jun 29, 2016Group: Database references
Revision 1.2Mar 20, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_oper_list / struct_conn
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_oper_list.symmetry_operation / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
B: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
C: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
D: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
E: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
F: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
G: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
H: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)497,97148
Polymers491,4708
Non-polymers6,50040
Water78,4914357
1
A: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
B: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)124,49312
Polymers122,8682
Non-polymers1,62510
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8880 Å2
ΔGint-49 kcal/mol
Surface area37850 Å2
MethodPISA
2
C: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
D: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)124,49312
Polymers122,8682
Non-polymers1,62510
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8850 Å2
ΔGint-47 kcal/mol
Surface area37860 Å2
MethodPISA
3
E: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
F: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)124,49312
Polymers122,8682
Non-polymers1,62510
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8890 Å2
ΔGint-45 kcal/mol
Surface area37750 Å2
MethodPISA
4
G: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
H: 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)124,49312
Polymers122,8682
Non-polymers1,62510
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area8890 Å2
ΔGint-48 kcal/mol
Surface area37690 Å2
MethodPISA
Unit cell
Length a, b, c (Å)90.791, 90.771, 169.561
Angle α, β, γ (deg.)83.27, 76.01, 64.30
Int Tables number1
Space group name H-MP1

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Components

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Protein , 1 types, 8 molecules ABCDEFGH

#1: Protein
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase / ThDP-dependent enzyme MenD / SEPHCHC synthase / Menaquinone biosynthesis protein MenD


Mass: 61433.781 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli K12 (bacteria) / Strain: K12 substr. MG1655 / Gene: menD, b2264, JW5374 / Plasmid: pET28a / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3)
References: UniProt: P17109, 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase

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Non-polymers , 5 types, 4397 molecules

#2: Chemical
ChemComp-TD6 / (4S)-4-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3lambda~5~-thiazol-2-yl}-4-hydroxybutanoic acid


Mass: 527.403 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C16H25N4O10P2S
#3: Chemical
ChemComp-MN / MANGANESE (II) ION


Mass: 54.938 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Mn
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: C3H8O3
#5: Chemical
ChemComp-FMT / FORMIC ACID


Mass: 46.025 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: CH2O2
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 4357 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.49 Å3/Da / Density % sol: 50.52 %
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop / pH: 7
Details: 0.16 M magnesium formate, 1% tascimate pH 7.0, 0.02 M HEPES pH 7.0, 14% PEG 3350 and 2% PEG MME 5000

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9792 Å
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 9, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 1.77→41.82 Å / Num. obs: 443335 / % possible obs: 96.6 % / Redundancy: 2 % / Rmerge(I) obs: 0.077 / Net I/σ(I): 7.1
Reflection shellResolution: 1.77→1.84 Å / Redundancy: 2 % / Rmerge(I) obs: 0.417 / % possible all: 94.8

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Processing

Software
NameVersionClassification
PHENIX1.9_1692refinement
iMOSFLMdata reduction
Aimlessdata scaling
PHASERphasing
RefinementResolution: 1.773→41.82 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 21.26 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2169 21846 4.93 %
Rwork0.1758 --
obs0.1778 443335 96.59 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.773→41.82 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms34485 0 392 4357 39234
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00736027
X-RAY DIFFRACTIONf_angle_d1.07649221
X-RAY DIFFRACTIONf_dihedral_angle_d13.59512993
X-RAY DIFFRACTIONf_chiral_restr0.0455436
X-RAY DIFFRACTIONf_plane_restr0.0056418
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.7731-1.79320.30726760.242213398X-RAY DIFFRACTION93
1.7932-1.81430.28537620.222614066X-RAY DIFFRACTION96
1.8143-1.83640.25777280.210513836X-RAY DIFFRACTION96
1.8364-1.85970.26977460.207813977X-RAY DIFFRACTION96
1.8597-1.88420.26637970.208113948X-RAY DIFFRACTION96
1.8842-1.910.25367450.195713872X-RAY DIFFRACTION96
1.91-1.93730.26416700.20114014X-RAY DIFFRACTION96
1.9373-1.96620.24167140.190514096X-RAY DIFFRACTION96
1.9662-1.99690.23617110.187113975X-RAY DIFFRACTION96
1.9969-2.02960.25057260.186614058X-RAY DIFFRACTION97
2.0296-2.06460.23367180.183914154X-RAY DIFFRACTION97
2.0646-2.10220.24417180.183214026X-RAY DIFFRACTION96
2.1022-2.14260.237570.173713957X-RAY DIFFRACTION97
2.1426-2.18630.22746310.173714131X-RAY DIFFRACTION97
2.1863-2.23390.21257790.170614031X-RAY DIFFRACTION97
2.2339-2.28580.23077000.175214198X-RAY DIFFRACTION97
2.2858-2.3430.22387570.174914094X-RAY DIFFRACTION97
2.343-2.40630.2167800.167314090X-RAY DIFFRACTION97
2.4063-2.47710.21757400.170414130X-RAY DIFFRACTION97
2.4771-2.55710.22047050.16914165X-RAY DIFFRACTION97
2.5571-2.64850.21397540.174314209X-RAY DIFFRACTION97
2.6485-2.75450.21827830.17514108X-RAY DIFFRACTION97
2.7545-2.87980.22497690.175414129X-RAY DIFFRACTION98
2.8798-3.03160.21647660.173314220X-RAY DIFFRACTION98
3.0316-3.22150.19987380.170314217X-RAY DIFFRACTION98
3.2215-3.47010.21696340.177314277X-RAY DIFFRACTION98
3.4701-3.81910.20357840.170414043X-RAY DIFFRACTION97
3.8191-4.37120.16927440.155413977X-RAY DIFFRACTION96
4.3712-5.50530.17766390.15714124X-RAY DIFFRACTION97
5.5053-41.83590.19096750.174413969X-RAY DIFFRACTION96
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
10.1901-0.22140.09370.2213-0.09210.1116-0.0208-0.00550.0180.02640.0321-0.0165-0.0180.01920.01880.0324-0.0047-0.01080.0702-0.01110.061618.66540.715222.6596
20.0902-0.05220.02670.0362-0.07020.0025-0.0365-0.061-0.0246-0.0678-0.01790.0215-0.0337-0.0617-0.00860.0642-0.0052-0.00470.1109-0.03320.1035-4.987938.473826.0943
30.2294-0.10280.25770.16280.07320.20530.0032-0.0345-0.03950.0332-0.00950.06980.0374-0.0399-0.00010.0598-0.01080.01530.10050.01650.1066-8.177412.75233.8537
40.20490.043-0.03050.0598-0.09080.12470.0049-0.0123-0.04020.0063-0.0028-0.00780.03460.0432-0.01180.05970.0121-0.00060.06780.01150.055415.391411.670939.3202
5-0.0178-0.0335-0.00880.1288-0.08880.27250.0031-0.0028-0.0089-0.01670.00850.0060.0410.02170.01020.05510.0068-0.00410.0896-0.01370.082219.97110.54189.9086
60.05510.0015-0.03660.00720.03610.16390.00960.0328-0.1044-0.0428-0.0191-0.12620.05230.078-0.00080.07320.0199-0.01530.1101-0.01930.121233.54713.532-7.6338
70.1233-0.07510.10260.0076-0.06780.08790.009-0.0370.021-0.0220.0176-0.0273-0.02240.0350.00850.05350.00140.01380.1208-0.00720.119650.645235.46483.7992
80.0610.01080.06950.00210.00770.0573-0.0392-0.0450.0189-0.01310.0287-0.05040.01820.077800.06010.0095-0.02260.1806-0.03070.150158.639931.13824.3625
90.12210.03950.04820.0453-0.04560.0916-0.0001-0.0058-0.01420.0101-0.0049-0.00920.0010.03630.00490.04420.0065-0.01210.1173-0.01750.085539.871525.045427.3644
100.1214-0.0085-0.0289-0.0066-0.00550.1542-0.0062-0.1720.00890.01310.0088-0.0193-0.01230.04080.00140.07630.021-0.02270.2204-0.02850.131650.101124.818333.3507
110.1213-0.045-0.00860.1403-0.06630.24740.01060.0341-0.0261-0.0234-0.01180.00190.0056-0.03240.00030.05010.0038-0.01470.0861-0.01850.0813-4.843323.6572-3.9015
120.066-0.01230.00390.0298-0.02990.07270.0382-0.0387-0.02780.0184-0.08540.059-0.0035-0.13640.00560.0572-0.01-0.00820.1316-0.00540.0921-13.994531.037515.6129
130.1714-0.04980.19720.1048-0.18040.2443-0.0164-0.01010.0404-0.0022-0.0016-0.0066-0.0837-0.0489-0.00430.05130.02-0.00820.0791-0.01250.0586-8.782160.088512.1622
140.0167-0.03080.00920.0895-0.02070.010.03830.02720.01550.0245-0.07620.0596-0.1236-0.2185-0.00480.12220.0639-0.00670.12650.01870.1277-15.193568.281-7.9036
150.1618-0.10170.03190.1697-0.0390.03920.03870.03550.0028-0.0788-0.0636-0.0075-0.0317-0.0114-0.00980.08980.0325-0.00750.11970.00880.0858-8.223254.7844-15.8275
160.1717-0.02460.09490.1055-0.14520.18740.02470.01030.0146-0.0245-0.0267-0.0252-0.0397-0.0053-0.00390.05910.00520.01830.08650.00410.083619.001645.5047-8.3421
170.1269-0.012-0.04270.0737-0.0490.03910.13710.09880.0146-0.0929-0.158-0.0643-0.01290.0505-0.00070.1010.02380.03680.1642-0.00540.125636.612831.752-12.301
180.2213-0.02290.1550.0135-0.07240.19910.07510.0596-0.0353-0.0747-0.0378-0.01760.09140.05230.00330.12740.04120.00020.1161-0.03120.079522.19959.5184-25.5536
190.1282-0.0371-0.05670.09350.05190.04940.05190.1435-0.0086-0.2544-0.0026-0.01710.1630.05130.02150.30240.063-0.05620.1861-0.08860.05128.241916.1023-42.0191
200.1216-0.13940.16690.09140.10430.20560.05530.0513-0.0269-0.0982-0.04170.01630.0151-0.01570.01550.12210.0394-0.01610.1312-0.02090.04972.391728.8974-32.1967
210.09210.08590.02240.2083-0.20310.15230.0060.01560.00580.05580.0072-0.0065-0.00580.03950.01990.0898-0.019500.0574-0.00730.056766.960946.4239-65.5819
220.02660.03540.00810.04820.04570.0585-0.0389-0.03840.07340.2550.0207-0.0129-0.12710.1194-0.0480.2332-0.03460.02090.0755-0.02730.128662.087568.2239-61.7016
230.06350.00680.09940.0786-0.09720.2020.0201-0.07170.02650.14020.06750.1728-0.0987-0.04850.01510.19570.02020.08560.0823-0.02010.052335.822561.2591-44.2809
240.01770.0315-0.07160.27580.02020.2734-0.0195-0.0165-0.00720.09660.0140.05330.0116-0.03180.00050.1808-0.00810.03510.10970.00390.085344.731438.7053-41.5477
250.16470.0644-0.01910.1545-0.10230.17450.017-0.02760.00640.0403-0.01110.03690.0081-0.0230.00230.0943-0.01830.02070.07310.00350.092936.833834.374-69.7522
260.037-0.0827-0.00930.0907-0.013-0.0285-0.0403-0.0505-0.07780.03050.02820.04990.10470.01250.00030.1114-0.0232-0.01090.0983-0.00850.113240.479522.5286-89.7345
270.05840.00490.17710.1773-0.13970.3225-0.00930.0144-0.0091-0.0007-0.0315-0.01420.09680.027400.09530.00210.01540.05910.00130.060567.692416.0821-85.1936
280.0646-0.08080.04110.1263-0.05680.0206-0.0287-0.0114-0.0221-0.0267-0.0192-0.0190.12790.0416-0.00030.17860.0266-0.00040.11540.01050.118471.92315.6366-72.1289
290.0534-0.0458-0.13770.1524-0.00260.1399-0.0181-0.0471-0.01060.07740.01930.01960.03890.0400.1276-0.0013-0.00280.07870.01020.077363.367217.8115-57.9147
300.1578-0.1174-0.04750.3334-0.04240.17850.02010.0038-0.0019-0.0384-0.0066-0.0087-0.01150.01880.01020.0754-0.02170.00690.05920.00360.067462.296448.3537-96.6152
310.03890.00530.04020.01970.0236-0.0021-0.00850.04820.0148-0.01110.01690.03080.04780.0220.00020.0873-0.01320.00460.0732-0.00010.096855.232126.9645-98.7285
320.14150.006-0.08760.271-0.15110.18470.00430.029-0.05-0.0193-0.01280.0640.0206-0.0538-0.00540.0737-0.0192-0.02110.0759-0.00580.111725.499435.6121-107.3523
330.1033-0.03990.05150.0984-0.09460.1125-0.00040.0190.0074-0.02070.00390.0588-0.0682-0.01630.00020.11080.0015-0.01560.0770.00050.091334.707757.8094-113.1135
34-0.0511-0.05320.09040.1782-0.06890.20810.0106-0.0209-0.01480.0043-0.00560.0335-0.0437-0.0146-0.00290.0751-0.01060.01260.0334-0.00240.064835.594462.3778-83.8179
350.05690.0436-0.09880.0276-0.0060.1512-0.0097-0.05280.10580.04110.00060.0484-0.0541-0.0139-00.1352-0.00230.02620.0762-0.01090.151644.682473.3786-66.0327
360.05550.05040.05070.146-0.06190.1768-0.0026-0.01370.02240.0186-0.001-0.0279-0.03350.0419-0.00310.1278-0.03740.01090.0923-0.00750.114471.479779.2469-77.6541
370.01540.03820.00690.15240.07280.04540.00110.0043-0.0299-0.09470.00960.0058-0.09140.056-0.0990.1307-0.07060.05050.08640.0070.134171.000688.2667-98.2466
380.06230.03850.04930.1332-0.08710.07960.00960.02130.0017-0.0275-0.00860.0102-0.03820.03990.00160.1327-0.03170.00730.07010.00020.092857.337374.1267-101.0564
390.02940.0214-0.05170.0755-0.03460.1251-0.06080.00050.0864-0.10540.1673-0.0862-0.03550.28450.00840.2208-0.11090.0606-0.09210.09620.076761.970883.1439-107.186
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1chain 'A' and (resid 1 through 182 )
2X-RAY DIFFRACTION2chain 'A' and (resid 183 through 230 )
3X-RAY DIFFRACTION3chain 'A' and (resid 231 through 384 )
4X-RAY DIFFRACTION4chain 'A' and (resid 385 through 556 )
5X-RAY DIFFRACTION5chain 'B' and (resid 1 through 182 )
6X-RAY DIFFRACTION6chain 'B' and (resid 183 through 219 )
7X-RAY DIFFRACTION7chain 'B' and (resid 220 through 347 )
8X-RAY DIFFRACTION8chain 'B' and (resid 348 through 384 )
9X-RAY DIFFRACTION9chain 'B' and (resid 385 through 503 )
10X-RAY DIFFRACTION10chain 'B' and (resid 504 through 556 )
11X-RAY DIFFRACTION11chain 'C' and (resid 1 through 182 )
12X-RAY DIFFRACTION12chain 'C' and (resid 183 through 219 )
13X-RAY DIFFRACTION13chain 'C' and (resid 220 through 347 )
14X-RAY DIFFRACTION14chain 'C' and (resid 348 through 384 )
15X-RAY DIFFRACTION15chain 'C' and (resid 385 through 556 )
16X-RAY DIFFRACTION16chain 'D' and (resid 1 through 182 )
17X-RAY DIFFRACTION17chain 'D' and (resid 183 through 219 )
18X-RAY DIFFRACTION18chain 'D' and (resid 220 through 347 )
19X-RAY DIFFRACTION19chain 'D' and (resid 348 through 384 )
20X-RAY DIFFRACTION20chain 'D' and (resid 385 through 556 )
21X-RAY DIFFRACTION21chain 'E' and (resid 1 through 182 )
22X-RAY DIFFRACTION22chain 'E' and (resid 183 through 219 )
23X-RAY DIFFRACTION23chain 'E' and (resid 220 through 384 )
24X-RAY DIFFRACTION24chain 'E' and (resid 385 through 556 )
25X-RAY DIFFRACTION25chain 'F' and (resid 1 through 182 )
26X-RAY DIFFRACTION26chain 'F' and (resid 183 through 219 )
27X-RAY DIFFRACTION27chain 'F' and (resid 220 through 336 )
28X-RAY DIFFRACTION28chain 'F' and (resid 337 through 384 )
29X-RAY DIFFRACTION29chain 'F' and (resid 385 through 556 )
30X-RAY DIFFRACTION30chain 'G' and (resid 1 through 182 )
31X-RAY DIFFRACTION31chain 'G' and (resid 183 through 219 )
32X-RAY DIFFRACTION32chain 'G' and (resid 220 through 384 )
33X-RAY DIFFRACTION33chain 'G' and (resid 385 through 556 )
34X-RAY DIFFRACTION34chain 'H' and (resid 1 through 182 )
35X-RAY DIFFRACTION35chain 'H' and (resid 183 through 219 )
36X-RAY DIFFRACTION36chain 'H' and (resid 220 through 347 )
37X-RAY DIFFRACTION37chain 'H' and (resid 348 through 384 )
38X-RAY DIFFRACTION38chain 'H' and (resid 385 through 503 )
39X-RAY DIFFRACTION39chain 'H' and (resid 504 through 556 )

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