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- PDB-5afh: alpha7-AChBP in complex with lobeline -

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Basic information

Entry
Database: PDB / ID: 5afh
Titlealpha7-AChBP in complex with lobeline
ComponentsACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
KeywordsTRANSPORT PROTEIN / PENTAMERIC LIGAND-GATED ION CHANNELS / CYS-LOOP RECEPTOR / NICOTINIC ACETYLCHOLINE RECEPTOR / ALLOSTERIC MODULATION / DRUG DISCOVERY
Function / homology
Function and homology information


sensory processing / dendrite arborization / regulation of synapse structural plasticity / synaptic transmission involved in micturition / activation of protein kinase C activity / response to acetylcholine / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / acetylcholine receptor activity / acetylcholine-gated channel complex / chloride channel regulator activity ...sensory processing / dendrite arborization / regulation of synapse structural plasticity / synaptic transmission involved in micturition / activation of protein kinase C activity / response to acetylcholine / Highly calcium permeable postsynaptic nicotinic acetylcholine receptors / acetylcholine receptor activity / acetylcholine-gated channel complex / chloride channel regulator activity / cholinergic synapse / regulation of amyloid fibril formation / acetylcholine-gated monoatomic cation-selective channel activity / short-term memory / acetylcholine receptor signaling pathway / dendritic spine organization / acetylcholine binding / regulation of amyloid precursor protein catabolic process / neurotransmitter receptor activity / positive regulation of amyloid-beta formation / synaptic transmission, cholinergic / postsynaptic specialization membrane / response to amyloid-beta / negative regulation of amyloid-beta formation / monoatomic ion transport / positive regulation of protein metabolic process / negative regulation of tumor necrosis factor production / modulation of excitatory postsynaptic potential / plasma membrane raft / monoatomic ion channel activity / toxic substance binding / axolemma / response to cold / negative regulation of cytokine production involved in inflammatory response / excitatory postsynaptic potential / positive regulation of excitatory postsynaptic potential / negative regulation of canonical NF-kappaB signal transduction / positive regulation of long-term synaptic potentiation / response to nicotine / memory / regulation of membrane potential / synapse organization / cognition / intracellular calcium ion homeostasis / calcium channel activity / positive regulation of angiogenesis / calcium ion transport / transmembrane signaling receptor activity / amyloid-beta binding / chemical synaptic transmission / presynaptic membrane / monoatomic ion transmembrane transport / response to hypoxia / learning or memory / positive regulation of ERK1 and ERK2 cascade / positive regulation of MAPK cascade / neuron projection / postsynapse / postsynaptic membrane / apical plasma membrane / external side of plasma membrane / positive regulation of cell population proliferation / dendrite / synapse / endoplasmic reticulum membrane / signal transduction / protein homodimerization activity / membrane / plasma membrane
Similarity search - Function
Nicotinic acetylcholine receptor / Acetylcholine Binding Protein; Chain: A, / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel ...Nicotinic acetylcholine receptor / Acetylcholine Binding Protein; Chain: A, / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel, conserved site / Neurotransmitter-gated ion-channels signature. / Neurotransmitter-gated ion-channel transmembrane domain / Neurotransmitter-gated ion-channel transmembrane region / Neurotransmitter-gated ion-channel transmembrane domain superfamily / Neuronal acetylcholine receptor / Neurotransmitter-gated ion-channel / Neurotransmitter-gated ion-channel ligand-binding domain / Neurotransmitter-gated ion-channel ligand-binding domain superfamily / Neurotransmitter-gated ion-channel ligand binding domain / Distorted Sandwich / Mainly Beta
Similarity search - Domain/homology
Alpha-Lobeline / Neuronal acetylcholine receptor subunit alpha-7
Similarity search - Component
Biological speciesHOMO SAPIENS (human)
LYMNAEA STAGNALIS (great pond snail)
MethodX-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.4 Å
AuthorsSpurny, R. / Debaveye, S. / Farinha, A. / Veys, K. / Gossas, T. / Atack, J. / Bertrand, D. / Kemp, J. / Vos, A. / Danielson, U.H. ...Spurny, R. / Debaveye, S. / Farinha, A. / Veys, K. / Gossas, T. / Atack, J. / Bertrand, D. / Kemp, J. / Vos, A. / Danielson, U.H. / Tresadern, G. / Ulens, C.
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2015
Title: Molecular Blueprint of Allosteric Binding Sites in a Homologue of the Agonist-Binding Domain of the Alpha7 Nicotinic Acetylcholine Receptor.
Authors: Spurny, R. / Debaveye, S. / Farinha, A. / Veys, K. / Vos, A.M. / Gossas, T. / Atack, J. / Bertrand, S. / Bertrand, D. / Danielson, U.H. / Tresadern, G. / Ulens, C.
History
DepositionJan 22, 2015Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 6, 2015Provider: repository / Type: Initial release
Revision 1.1May 13, 2015Group: Database references
Revision 1.2May 27, 2015Group: Database references
Revision 1.3Jun 10, 2015Group: Atomic model / Derived calculations / Other
Revision 1.4Mar 15, 2017Group: Source and taxonomy
Revision 1.5Apr 24, 2019Group: Data collection / Derived calculations / Source and taxonomy
Category: entity_src_gen / struct_conn
Item: _entity_src_gen.pdbx_host_org_cell_line / _struct_conn.pdbx_leaving_atom_flag
Revision 2.0Jul 29, 2020Group: Advisory / Atomic model ...Advisory / Atomic model / Data collection / Derived calculations / Other / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / chem_comp / database_PDB_caveat / entity / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_database_status / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / pdbx_validate_chiral / pdbx_validate_close_contact / pdbx_validate_symm_contact / struct_asym / struct_conn / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _atom_site_anisotrop.id / _atom_site_anisotrop.pdbx_auth_asym_id / _atom_site_anisotrop.pdbx_auth_seq_id / _atom_site_anisotrop.pdbx_label_asym_id / _chem_comp.name / _chem_comp.type / _pdbx_database_status.status_code_sf / _pdbx_struct_assembly_gen.asym_id_list / _pdbx_validate_chiral.auth_asym_id / _pdbx_validate_chiral.auth_seq_id / _pdbx_validate_close_contact.auth_asym_id_1 / _pdbx_validate_close_contact.auth_asym_id_2 / _pdbx_validate_close_contact.auth_seq_id_1 / _pdbx_validate_close_contact.auth_seq_id_2 / _pdbx_validate_symm_contact.auth_asym_id_1 / _pdbx_validate_symm_contact.auth_seq_id_1 / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.pdbx_role / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_comp_id
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 16, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_entry_details / pdbx_modification_feature / struct_conn
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_entry_details.has_protein_modification / _struct_conn.pdbx_leaving_atom_flag

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
B: ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
C: ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
D: ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
E: ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7
hetero molecules


Theoretical massNumber of molelcules
Total (without water)122,62315
Polymers118,4895
Non-polymers4,13410
Water7,206400
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
MethodPISA
Unit cell
Length a, b, c (Å)85.520, 112.504, 143.109
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

#1: Protein
ACETYLCHOLINE-BINDING PROTEIN, NEURONAL ACETYLCHOLINE RECEPTOR SUBUNIT ALPHA-7 / ALPHA7-ACHBP / ACH-BINDING PROTEIN / ACHBP


Mass: 23697.840 Da / Num. of mol.: 5
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) HOMO SAPIENS (human), (gene. exp.) LYMNAEA STAGNALIS (great pond snail)
Cell line (production host): Sf21 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: P36544*PLUS
#2: Polysaccharide alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1- ...alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 748.682 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpa1-3DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/3,4,3/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5][a1122h-1a_1-5]/1-1-2-3/a4-b1_b4-c1_c3-d1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{[(3+1)][a-D-Manp]{}}}}}LINUCSPDB-CARE
#3: Polysaccharide
2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 424.401 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DGlcpNAcb1-4DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1b_1-5_2*NCC/3=O]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{}}}LINUCSPDB-CARE
#4: Chemical
ChemComp-L0B / Alpha-Lobeline


Mass: 337.455 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: C22H27NO2
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 400 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY
Sequence detailsCHIMERIC PROTEIN BASED ON UNP ENTRIES P58154, P36544 MULTIPLE REGIONS OF THE CONSTRUCT EITHER ...CHIMERIC PROTEIN BASED ON UNP ENTRIES P58154, P36544 MULTIPLE REGIONS OF THE CONSTRUCT EITHER CORRESPOND TO HUMAN OR SNAIL.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION

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Sample preparation

CrystalDensity Matthews: 2.91 Å3/Da / Density % sol: 57.66 % / Description: NONE

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.9194
DetectorType: ADSC CCD / Detector: CCD / Date: May 21, 2014
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9194 Å / Relative weight: 1
ReflectionResolution: 2.7→48.59 Å / Num. obs: 38863 / % possible obs: 99.6 % / Observed criterion σ(I): 1.5 / Redundancy: 4.4 % / Biso Wilson estimate: 52.85 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 16.7

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Processing

SoftwareName: PHENIX / Version: (PHENIX.REFINE) / Classification: refinement
RefinementMethod to determine structure: OTHER / Resolution: 2.4→44.223 Å / SU ML: 0.3 / σ(F): 1.35 / Phase error: 25.71 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2272 2715 5 %
Rwork0.168 --
obs0.1709 54489 99.65 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 2.4→44.223 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8345 0 287 400 9032
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0098887
X-RAY DIFFRACTIONf_angle_d1.22912056
X-RAY DIFFRACTIONf_dihedral_angle_d16.423275
X-RAY DIFFRACTIONf_chiral_restr0.0461336
X-RAY DIFFRACTIONf_plane_restr0.0051515
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.4-2.44370.33791330.28552683X-RAY DIFFRACTION99
2.4437-2.49070.31681460.26862668X-RAY DIFFRACTION100
2.4907-2.54150.29471350.25752690X-RAY DIFFRACTION100
2.5415-2.59680.31971220.24342750X-RAY DIFFRACTION100
2.5968-2.65720.33971260.23982674X-RAY DIFFRACTION100
2.6572-2.72360.32931560.22542683X-RAY DIFFRACTION99
2.7236-2.79720.30321610.23932673X-RAY DIFFRACTION100
2.7972-2.87950.33261470.21342716X-RAY DIFFRACTION100
2.8795-2.97240.2511450.20192691X-RAY DIFFRACTION100
2.9724-3.07870.25761480.1882717X-RAY DIFFRACTION100
3.0787-3.20190.28911680.18982690X-RAY DIFFRACTION100
3.2019-3.34760.25491500.18052693X-RAY DIFFRACTION100
3.3476-3.5240.2671130.16732770X-RAY DIFFRACTION100
3.524-3.74470.22751330.15222728X-RAY DIFFRACTION100
3.7447-4.03360.20021320.14612767X-RAY DIFFRACTION100
4.0336-4.43920.16261300.12682741X-RAY DIFFRACTION100
4.4392-5.08080.1851730.12492747X-RAY DIFFRACTION100
5.0808-6.39810.20641450.15172790X-RAY DIFFRACTION100
6.3981-44.23030.17171520.16022903X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.8733-0.43620.05152.02490.09982.3166-0.0134-0.10630.0654-0.00590.0355-0.2603-0.29520.2837-0.03860.3567-0.06330.02610.3393-0.00110.3359-2.013617.3819-16.2157
21.3902-0.0631-0.40411.8936-0.26072.4878-0.0733-0.2503-0.02050.23080.0346-0.0859-0.00910.11840.05920.3870.07530.01340.43890.00090.3306-11.45784.22245.5804
32.4032-0.44410.31591.85170.04731.7116-0.1507-0.2641-0.26820.19810.07620.23610.5463-0.19450.08280.4757-0.0150.15050.42740.02980.4505-24.5089-17.8872-3.0443
42.4355-1.0549-0.23091.91860.09932.4223-0.01160.138-0.238-0.1884-0.07610.170.3789-0.14420.07630.4021-0.06130.0170.3121-0.03050.3747-23.3708-18.6127-30.0144
51.6029-0.6484-0.35632.15960.14641.67690.00940.07090.0359-0.4244-0.0227-0.0784-0.06080.08070.01660.419-0.01040.01440.2911-0.00840.2974-9.57643.1782-38.2416
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A
2X-RAY DIFFRACTION2CHAIN B
3X-RAY DIFFRACTION3CHAIN C
4X-RAY DIFFRACTION4CHAIN D
5X-RAY DIFFRACTION5CHAIN E

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