+Open data
-Basic information
Entry | Database: PDB / ID: 5a9p | |||||||||
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Title | Crystal structure of Operophtera brumata CPV18 polyhedra | |||||||||
Components | POLYHEDRIN | |||||||||
Keywords | VIRAL PROTEIN / INSECT VIRUS OCCLUSION BODY / MICROCRYSTAL | |||||||||
Function / homology | Cypovirus polyhedrin, Cypovirus 1 type / Cypovirus polyhedrin protein / GTP binding / ATP binding / ADENOSINE-5'-TRIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Polyhedrin Function and homology information | |||||||||
Biological species | OPEROPHTERA BRUMATA CYPOVIRUS 18 | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.476 Å | |||||||||
Authors | Ji, X. / Axford, D. / Owen, R. / Evans, G. / Ginn, H.M. / Sutton, G. / Stuart, D.I. | |||||||||
Citation | Journal: J.Struct.Biol. / Year: 2015 Title: Polyhedra Structures and the Evolution of the Insect Viruses. Authors: Ji, X. / Axford, D. / Owen, R. / Evans, G. / Ginn, H.M. / Sutton, G. / Stuart, D.I. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5a9p.cif.gz | 150.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5a9p.ent.gz | 119.7 KB | Display | PDB format |
PDBx/mmJSON format | 5a9p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a9/5a9p ftp://data.pdbj.org/pub/pdb/validation_reports/a9/5a9p | HTTPS FTP |
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-Related structure data
Related structure data | 5a8sC 5a8tC 5a8uC 5a8vC 5a96C 5a98C 5a99C 5a9aC 5a9bC 5a9cC 2oh6S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 28406.531 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) OPEROPHTERA BRUMATA CYPOVIRUS 18 / Cell line (production host): SF9 / Production host: SPODOPTERA FRUGIPERDA (fall armyworm) / References: UniProt: Q30C70 |
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#2: Chemical | ChemComp-ATP / |
#3: Chemical | ChemComp-GTP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 21 |
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-Sample preparation
Crystal | Description: NONE |
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Crystal grow | pH: 7.5 / Details: PH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I24 / Wavelength: 0.979 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 1.48→72.7 Å / Num. obs: 30415 / % possible obs: 100 % / Observed criterion σ(I): 1 / Redundancy: 12.9 % / Rmerge(I) obs: 0.32 / Net I/σ(I): 7.1 |
Reflection shell | Resolution: 1.48→1.51 Å / Redundancy: 8.6 % / Mean I/σ(I) obs: 1.5 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2OH6 Resolution: 1.476→72.669 Å / SU ML: 0.13 / σ(F): 1.34 / Phase error: 13.71 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.476→72.669 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 34.1141 Å / Origin y: 21.1181 Å / Origin z: 8.7019 Å
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Refinement TLS group | Selection details: ALL |