- PDB-4wy2: Crystal structure of universal stress protein E from Proteus mira... -
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基本情報
登録情報
データベース: PDB / ID: 4wy2
タイトル
Crystal structure of universal stress protein E from Proteus mirabilis in complex with UDP-3-O-[(3R)-3-hydroxytetradecanoyl]-N-acetyl-alpha-glucosamine
要素
Universal stress protein E
キーワード
STRUCTURAL GENOMICS / UNKNOWN FUNCTION / MCSG / PSI-Biology / Midwest Center for Structural Genomics
機能・相同性
Rossmann fold - #12370 / UspA / Universal stress protein family / Rossmann fold / 3-Layer(aba) Sandwich / cytoplasm / Alpha Beta / Chem-U20 / Universal stress protein E
ジャーナル: to be published タイトル: Crystal structure of universal stress protein E from Proteus mirabilis incomplex withUDP-3-O-[(3R)-3-hydroxytetradecanoyl]-N-acetyl-alpha-glucosamine 著者: Shumilin, I.A. / Minor, W.
温度: 289 K / 手法: 蒸気拡散法 / pH: 6.5 詳細: 0.2 ul of 12 mg/ml protein in 10mM HEPES, 500mM NaCl pH 7.5 were mixed with 0.2 ul of the Quiagen Cryos Suite condition #50 (1.36 M Ammonium Sulfate, 0.085 M MES pH 6.5, 8.5% Dioxane, 15% ...詳細: 0.2 ul of 12 mg/ml protein in 10mM HEPES, 500mM NaCl pH 7.5 were mixed with 0.2 ul of the Quiagen Cryos Suite condition #50 (1.36 M Ammonium Sulfate, 0.085 M MES pH 6.5, 8.5% Dioxane, 15% Glycerol) and equilibrated against the mother liquor in 96 Well 2 drop Crystallization Plate (MRC) PH範囲: 6.5-7.5
解像度: 1.8→50 Å / Cor.coef. Fo:Fc: 0.969 / Cor.coef. Fo:Fc free: 0.955 / WRfactor Rfree: 0.2066 / WRfactor Rwork: 0.1721 / FOM work R set: 0.8453 / SU B: 4.761 / SU ML: 0.077 / SU R Cruickshank DPI: 0.1016 / SU Rfree: 0.1003 / 交差検証法: THROUGHOUT / σ(F): 0 / ESU R: 0.102 / ESU R Free: 0.1 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.2018
2019
4.9 %
RANDOM
Rwork
0.1721
39066
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obs
0.1736
39066
98.99 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK