THE ASSEMBLY REPRESENTED IN THIS ENTRY HAS IRREGULAR HELICAL SYMMETRY WITH THE FOLLOWING AVERAGE PARAMETERS: ROTATION PER DIMER (TWIST) = -75.00 DEGREES RISE PER DIMER (HEIGHT) = 9.51 ANGSTROMS
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要素
#1: タンパク質
ORF73 / LATENCY-ASSOCIATED NUCLEAR ANTIGEN / LANA-1 / KSHV LANA
マシュー密度: 2.79 Å3/Da / 溶媒含有率: 55.88 % 解説: DATA IN RESOLUTION RANGE 3.93 A - 3.87 A IS EXCLUDED DUE TO THE PRESENCE OF AN ICE RING
結晶化
温度: 293 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.5 詳細: 0.4 UL OF 31.5 MG/ML PROTEIN IN 5 MM BISTRIS-CL, 5 MM DTT, PH 6.5 WERE AS SUCH EQUILIBRATED AGAINST A RESERVOIR SOLUTION OF 170 MM LITHIUM ACETATE AND 18% (W/V) PEG3350 IN A SITTING DROP ...詳細: 0.4 UL OF 31.5 MG/ML PROTEIN IN 5 MM BISTRIS-CL, 5 MM DTT, PH 6.5 WERE AS SUCH EQUILIBRATED AGAINST A RESERVOIR SOLUTION OF 170 MM LITHIUM ACETATE AND 18% (W/V) PEG3350 IN A SITTING DROP SETUP AT 20 DEGREE CENTIGRADE. AFTER TWO DAYS, CRYSTALS WERE DETACHED FROM THE CARRIER PLASTIC BY ADDING 1 UL OF 2 M AMMONIUM FORMATE.
解像度: 3.7→89.36 Å / Cor.coef. Fo:Fc: 0.936 / Cor.coef. Fo:Fc free: 0.908 / SU B: 40.346 / SU ML: 0.553 / 交差検証法: THROUGHOUT / ESU R Free: 0.692 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.2386
389
4.9 %
RANDOM
Rwork
0.20078
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-
-
obs
0.20278
7485
95.83 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK