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- PDB-4uz8: The SeMet structure of the family 46 carbohydrate-binding module ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4uz8 | ||||||
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Title | The SeMet structure of the family 46 carbohydrate-binding module (CBM46) of endo-beta-1,4-glucanase B (Cel5B) from Bacillus halodurans | ||||||
![]() | ENDO-BETA-1,4-GLUCANASE (CELULASE B) | ||||||
![]() | SUGAR BINDING PROTEIN / CARBOHYDRATE BINDING PROTEIN / CARBOHYDRATE-BINDING MODULE FAMILY 46 / CBM46 / CEL5B / BACILLUS HALODURANS / SEMET DERIVATIVE | ||||||
Function / homology | ![]() glucan catabolic process / beta-glucosidase activity / cell surface / extracellular region / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Venditto, I. / Santos, H. / Ferreira, L.M.A. / Sakka, K. / Fontes, C.M.G.A. / Najmudin, S. | ||||||
![]() | ![]() Title: Family 46 Carbohydrate-Binding Modules Contribute to the Enzymatic Hydrolysis of Xyloglucan and Beta-1,3-1,4-Glucans Through Distinct Mechanisms. Authors: Venditto, I. / Najmudin, S. / Luis, A.S. / Ferreira, L.M. / Sakka, K. / Knox, J.P. / Gilbert, H.J. / Fontes, C.M. #1: Journal: Acta Crystallogr.,Sect.F / Year: 2014 Title: Overproduction, Purification, Crystallization and Preliminary X-Ray Characterization of the Family 46 Carbohydrate-Binding Module (Cbm46) of Endo-Beta-1,4-Glucanase B (Celb) from Bacillus Halodurans Authors: Venditto, I. / Santos, H. / Ferreira, L.M.A. / Sakka, K. / Fontes, C.M.G.A. / Najmudin, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.3 KB | Display | ![]() |
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PDB format | ![]() | 82.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 453.3 KB | Display | ![]() |
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Full document | ![]() | 459 KB | Display | |
Data in XML | ![]() | 11.6 KB | Display | |
Data in CIF | ![]() | 15.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4uznC ![]() 4v2xC ![]() 4uzp C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 14514.273 Da / Num. of mol.: 2 Fragment: CARBOHYDRATE BINDING MODULE FAMILY 46, UNP RESIDUES 457-563 Source method: isolated from a genetically manipulated source Details: SELENOMETHIONYLATED DERIVATIVE / Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-SO4 / | #3: Water | ChemComp-HOH / | Nonpolymer details | SULFATE ION (SO4): FROM CRYSTALLIS | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50 % / Description: NONE |
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Crystal grow | pH: 7 Details: 0.2 M AMMONIUM SULFATE, 30% W/V POLYETHYLENE GLYCOL 4,000, pH 7.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 6, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97976 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→85.45 Å / Num. obs: 12870 / % possible obs: 100 % / Observed criterion σ(I): 0 / Redundancy: 19.1 % / Rmerge(I) obs: 0.12 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 2.3→2.38 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.8 / Mean I/σ(I) obs: 1.3 / % possible all: 99.6 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: NONE Resolution: 2.3→85.45 Å / Cor.coef. Fo:Fc: 0.945 / Cor.coef. Fo:Fc free: 0.92 / SU B: 17.115 / SU ML: 0.202 / Cross valid method: THROUGHOUT / ESU R: 0.276 / ESU R Free: 0.225 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES WITH TLS ADDED. THE COORDINATE FILE WA REFINED BY PDB_REDO IN THE PENULTIMATE ROOUND OF REFINEMENT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.834 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→85.45 Å
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Refine LS restraints |
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