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- PDB-4s37: Crystal structure of R2 pyocin membrane-piercing spike -

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Open data

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Basic information

Entry

Database: PDB / ID: 4s37

Title

Crystal structure of R2 pyocin membrane-piercing spike

Components

Phage baseplate protein

Keywords

METAL BINDING PROTEIN / Cell Puncturing Device / Trimer / beta-helix / Iron-binding / Outer cell membrane piercing

Function / homology

Phage baseplate assembly protein V/Gp45 / Gp5/Type VI secretion system Vgr protein, OB-fold domain / Type VI secretion system/phage-baseplate injector OB domain / Vgr protein, OB-fold domain superfamily / metal ion binding / TRIETHYLENE GLYCOL / Baseplate assembly protein V

Function and homology information

Biological species

Pseudomonas aeruginosa (bacteria)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.2 Å

Authors

Browning, C.B. / Leiman, P.G. / Shneider, M.M.

Citation

Journal: To be Published
Title: Crystal structure of R2 pyocin membrane-piercing spike
Authors: Browning, C.B. / Leiman, P.G. / Shneider, M.M.

History

Deposition	Jan 26, 2015	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Jan 27, 2016	Provider: repository / Type: Initial release
Revision 1.1	Nov 22, 2017	Group: Advisory / Refinement description / Category: pdbx_unobs_or_zero_occ_atoms / software / Item: _software.name
Revision 1.2	Feb 28, 2024	Group: Advisory / Data collection ...Advisory / Data collection / Database references / Derived calculations Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_unobs_or_zero_occ_atoms / struct_conn / struct_site Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewer	Molecule: MolmilJmol/JSmol

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Downloads & links

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Download

PDBx/mmCIF format	4s37.cif.gz	1.2 MB	Display	PDBx/mmCIF format
PDB format	pdb4s37.ent.gz	1 MB	Display	PDB format
PDBx/mmJSON format	4s37.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	4s37_validation.pdf.gz	620 KB	Display	wwPDB validaton report
Full document	4s37_full_validation.pdf.gz	651.9 KB	Display
Data in XML	4s37_validation.xml.gz	137.4 KB	Display
Data in CIF	4s37_validation.cif.gz	191.9 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/s3/4s37 ftp://data.pdbj.org/pub/pdb/validation_reports/s3/4s37	HTTPS FTP

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Related structure data

Related structure data	4s36
Similar structure data	2hbh-1 1ydw-d 4afu-d 5nkq-1 1wqw-d 5thg-2 5nkq-2 5nma-1 Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Search similar-shape structures by Omokage search (details) Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

Related structure data

4s36

Similar structure data

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Links

PDB pages	PDBj / wwPDB / NCBI

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Assembly

Deposited unit

A: Phage baseplate protein

B: Phage baseplate protein

C: Phage baseplate protein

D: Phage baseplate protein

E: Phage baseplate protein

F: Phage baseplate protein

G: Phage baseplate protein

H: Phage baseplate protein

I: Phage baseplate protein

J: Phage baseplate protein

K: Phage baseplate protein

L: Phage baseplate protein

M: Phage baseplate protein

N: Phage baseplate protein

O: Phage baseplate protein

P: Phage baseplate protein

Q: Phage baseplate protein

R: Phage baseplate protein

hetero molecules

355 kDa, 18 polymers
Search similar-shape structures of this assembly by Omokage search (details)

A: Phage baseplate protein

B: Phage baseplate protein

C: Phage baseplate protein

hetero molecules

defined by author&software
59.2 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

D: Phage baseplate protein

E: Phage baseplate protein

F: Phage baseplate protein

hetero molecules

defined by author&software
59.2 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

G: Phage baseplate protein

H: Phage baseplate protein

I: Phage baseplate protein

hetero molecules

defined by author&software
59.4 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

J: Phage baseplate protein

K: Phage baseplate protein

L: Phage baseplate protein

hetero molecules

defined by author&software
59.3 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

M: Phage baseplate protein

N: Phage baseplate protein

O: Phage baseplate protein

hetero molecules

defined by author&software
59.1 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

P: Phage baseplate protein

Q: Phage baseplate protein

R: Phage baseplate protein

hetero molecules

defined by author&software
58.6 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	94.945, 137.471, 164.759
Angle α, β, γ (deg.)	90.00, 106.56, 90.00
Int Tables number	4
Space group name H-M	P12₁1

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Components

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Protein , 1 types, 18 molecules ABCDEFGHIJKLMNOPQR

#1: Protein	Phage baseplate protein / VR2 protein Mass: 19443.758 Da / Num. of mol.: 18 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa (bacteria) Gene: VR2, gpV, D480_28305, JF43_02945, NCGM1900_0632, NCGM1984_0630 Plasmid: pET-28-based / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9S581

#1: Protein

Phage baseplate protein / VR2 protein

Mass: 19443.758 Da / Num. of mol.: 18
Source method: isolated from a genetically manipulated source
Source: (gene. exp.)

Pseudomonas aeruginosa (bacteria)
Gene: VR2, gpV, D480_28305, JF43_02945, NCGM1900_0632, NCGM1984_0630
Plasmid: pET-28-based / Production host:

Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9S581

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Non-polymers , 6 types, 2501 molecules

#2: Chemical	... ChemComp-SO4 / SULFATE ION Mass: 96.063 Da / Num. of mol.: 35 / Source method: obtained synthetically / Formula: SO₄
#3: Chemical	ChemComp-CL / CHLORIDE ION Mass: 35.453 Da / Num. of mol.: 19 / Source method: obtained synthetically / Formula: Cl
#4: Chemical	ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL Mass: 62.068 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C₂H₆O₂
#5: Chemical	ChemComp-CA / CALCIUM ION Mass: 40.078 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: Ca
#6: Chemical	ChemComp-PGE / TRIETHYLENE GLYCOL Mass: 150.173 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C₆H₁₄O₄
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 2433 / Source method: isolated from a natural source / Formula: H₂O

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Experimental details

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.94 Å³/Da / Density % sol: 58.23 %
Crystal grow	Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 60% PEG 400, 100mM NaSO4, 100mM Bis-Tris, pH 8.5, 18mg/ml protein, VAPOR DIFFUSION, HANGING DROP, temperature 291K

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Data collection

Diffraction	Mean temperature: 100 K
Diffraction source	Source: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å
Detector	Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 7, 2011
Radiation	Monochromator: Si (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1 Å / Relative weight: 1
Reflection	Resolution: 2.2→49.26 Å / Num. obs: 203160 / % possible obs: 98.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Redundancy: 4.4 % / R_merge(I) obs: 0.05 / R_sym value: 0.063 / Net I/σ(I): 15.1
Reflection shell	Resolution: 2.2→2.32 Å / Redundancy: 4 % / R_merge(I) obs: 0.414 / Mean I/σ(I) obs: 3.2 / Num. unique all: 28488 / R_sym value: 0.467 / % possible all: 95.3

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Processing

Software

Name	Version	Classification
RemDAq		data collection
PHASER		phasing
PHENIX	(phenix.refine: 1.8.2_1309)	refinement
XDS		data reduction
SCALA		data scaling

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.2→49.186 Å / SU ML: 0.24 / σ(F): 2 / Phase error: 19.62 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2079	10157	5 %
R_work	0.1625	-	-
obs	0.1648	203160	99.12 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.2→49.186 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	22839	0	238	2433	25510

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.004	24564
X-RAY DIFFRACTION	f_angle_d	0.889	33604
X-RAY DIFFRACTION	f_dihedral_angle_d	10.708	8652
X-RAY DIFFRACTION	f_chiral_restr	0.057	3742
X-RAY DIFFRACTION	f_plane_restr	0.003	4351

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.2-2.2256	0.2875	291	0.2307	5536	X-RAY DIFFRACTION	86
2.2256-2.2517	0.2497	340	0.204	6449	X-RAY DIFFRACTION	100
2.2517-2.2792	0.263	338	0.2016	6421	X-RAY DIFFRACTION	100
2.2792-2.3081	0.2545	342	0.1932	6498	X-RAY DIFFRACTION	100
2.3081-2.3384	0.2339	338	0.1857	6418	X-RAY DIFFRACTION	100
2.3384-2.3705	0.224	340	0.1767	6465	X-RAY DIFFRACTION	100
2.3705-2.4043	0.2328	338	0.177	6427	X-RAY DIFFRACTION	100
2.4043-2.4402	0.2157	343	0.1721	6518	X-RAY DIFFRACTION	100
2.4402-2.4783	0.2437	338	0.1742	6414	X-RAY DIFFRACTION	100
2.4783-2.519	0.2325	339	0.166	6440	X-RAY DIFFRACTION	100
2.519-2.5624	0.2263	342	0.1668	6497	X-RAY DIFFRACTION	100
2.5624-2.609	0.2143	341	0.1652	6488	X-RAY DIFFRACTION	100
2.609-2.6592	0.2307	340	0.1642	6459	X-RAY DIFFRACTION	100
2.6592-2.7134	0.2263	340	0.1698	6462	X-RAY DIFFRACTION	100
2.7134-2.7724	0.2191	338	0.1735	6421	X-RAY DIFFRACTION	100
2.7724-2.8369	0.2376	341	0.1705	6477	X-RAY DIFFRACTION	100
2.8369-2.9079	0.2384	342	0.1776	6486	X-RAY DIFFRACTION	100
2.9079-2.9865	0.2279	341	0.1694	6486	X-RAY DIFFRACTION	100
2.9865-3.0743	0.2241	340	0.1653	6457	X-RAY DIFFRACTION	100
3.0743-3.1736	0.1974	340	0.1602	6461	X-RAY DIFFRACTION	100
3.1736-3.287	0.1994	340	0.1609	6454	X-RAY DIFFRACTION	100
3.287-3.4185	0.2094	337	0.1575	6429	X-RAY DIFFRACTION	99
3.4185-3.5741	0.2085	340	0.1562	6458	X-RAY DIFFRACTION	99
3.5741-3.7624	0.1974	341	0.1512	6479	X-RAY DIFFRACTION	100
3.7624-3.9981	0.1775	341	0.1438	6464	X-RAY DIFFRACTION	99
3.9981-4.3066	0.1733	340	0.1334	6471	X-RAY DIFFRACTION	99
4.3066-4.7397	0.1699	339	0.1261	6411	X-RAY DIFFRACTION	98
4.7397-5.4248	0.1859	339	0.1429	6454	X-RAY DIFFRACTION	99
5.4248-6.8317	0.1993	345	0.1659	6553	X-RAY DIFFRACTION	100
6.8317-49.198	0.2313	343	0.2116	6550	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.2264	-0.3828	-0.362	0.6312	0.5818	0.5334	0.16	-0.1234	-0.2837	0.1154	0.0604	-0.3077	0.7797	0.1988	0.3374	0.4999	0.0512	-0.1339	0.2386	0.0559	0.4552	14.8425	-13.8236	74.2575
2	0.4804	0.4486	-0.1022	0.4591	-0.0578	0.0725	0.1327	-0.0371	-0.1704	0.0624	0.0203	-0.1103	0.1773	0.0257	0.0018	0.3483	0.0078	-0.0794	0.2231	0.0175	0.2368	9.9733	-2.9577	73.8254
3	0.0462	0.0913	0.0535	0.157	0.0977	0.0862	-0.1333	-0.1186	-0.1463	0.8822	0.0063	0.0022	0.1392	0.1516	0.0009	0.4593	-0.0694	-0.006	0.3607	0.0436	0.3337	7.5539	13.6438	81.3519
4	0.1893	0.1389	0.0038	0.2622	0.1037	0.0566	0.2367	-0.0149	0.1477	0.7923	-0.0879	0.5518	-0.1228	0.0144	-0.0018	0.4067	-0.0763	0.057	0.2658	0.069	0.3349	1.3606	20.1879	81.5833
5	0.035	0.0051	0.0264	0.0073	-0.0136	0.0334	0.2517	0.1976	-0.2585	0.0917	0.1221	-0.307	0.1139	0.1184	-0	0.4083	-0.0563	-0.109	0.3522	0.0346	0.3665	17.4753	22.9191	74.2801
6	1.2907	0.3831	0.2783	0.1594	0.1563	0.1732	-0.1119	0.3457	-0.3397	-0.0352	-0.0872	-0.873	-0.1399	0.2429	-0.1732	0.3738	-0.0812	-0.1096	0.3089	0.0955	0.3612	18.7035	29.6399	72.5451
7	0.0141	0.0534	-0.0305	0.3238	-0.1628	0.0762	-0.0627	0.5145	0.0147	0.3633	-0.0714	0.5062	-0.2197	0.0035	-0.0078	0.3021	-0.0345	-0.0022	0.3682	0.0208	0.3842	9.3956	40.4334	67.7536
8	0.0288	-0.1283	0.0252	0.6122	-0.1054	0.0229	-0.1042	0.0417	-0.099	0.8646	0.1627	0.0792	-0.1948	0.1356	0.0034	0.5578	-0.0272	-0.0682	0.345	0.052	0.2829	16.8735	46.1346	77.5972
9	0.3402	-0.1972	-0.2224	0.2634	0.0091	0.7201	0.1551	-0.188	0.3067	0.7075	0.3046	-0.0869	-0.5153	0.4943	0.0192	0.5945	-0.0807	-0.056	0.3211	0.0436	0.3799	12.9913	56.2698	73.1276
10	0.3298	0.1923	-0.1376	0.1274	-0.0869	0.069	0.1427	-0.2501	-0.2074	0.2855	0.0217	0.2805	0.3977	-0.1124	0.1689	0.4647	-0.0773	-0.053	0.2377	0.0874	0.3891	-6.8395	-11.3103	81.6347
11	0.4696	0.4164	-0.3078	0.7883	0.1044	0.3894	0.0567	-0.0354	0.0285	0.1193	0.0555	0.2586	0.1501	-0.0309	-0	0.3459	-0.0324	-0.0339	0.279	0.0841	0.3598	-1.4747	4.0442	74.1504
12	0.0011	-0.0106	-0.0002	0.0706	-0.0477	0.0362	0.0177	0.1837	-0.2029	-0.3058	0.0639	0.3182	0.0943	-0.0163	-0.0002	0.4165	-0.0266	-0.081	0.3428	0.032	0.4616	3.5434	20.2755	62.2199
13	0.2716	0.3561	0.0089	0.9314	-0.2151	0.0983	0.2031	-0.3298	0.108	1.2049	-0.175	0.2755	-0.2697	-0.0761	-0.0238	0.5021	-0.0605	0.0355	0.303	0.0417	0.3589	5.8102	29.5467	80.0524
14	0.4244	0.0532	0.3317	0.4437	-0.4617	0.8028	-0.0825	-0.0587	0.2538	0.638	-0.1339	-0.2503	-0.5712	0.2288	-0.1719	0.4184	-0.1022	-0.0558	0.3178	0.0693	0.256	15.2178	47.4458	73.046
15	0.2982	0.0619	-0.0452	0.1795	-0.0456	0.0268	0.1741	0.2371	-0.1613	-0.3464	0.0764	0.5766	0.5419	-0.0664	0.0217	0.6195	-0.079	-0.1124	0.2726	0.0198	0.3064	-1.5407	-15.9629	61.4498
16	0.3397	0.3959	-0.1141	0.9589	-0.0934	0.0521	-0.1331	0.1796	-0.1221	-0.1033	0.4054	0.2617	0.4698	-0.0975	0.3763	0.4773	-0.0932	-0.1887	0.2945	0.0324	0.3518	-4.625	-6.1331	56.2539
17	0.3181	0.1402	0.2251	0.7184	-0.1436	0.2281	0.0146	0.1033	-0.0965	-0.2023	0.0756	0.0601	0.0737	-0.0902	0.0233	0.323	-0.0606	-0.0655	0.2116	0.008	0.2148	1.9246	-0.7441	64.9271
18	0.0199	-0.0588	0.0017	0.1298	-0.0004	0.0007	-0.0699	0.1162	-0.1091	0.0082	0.1138	-0.1755	-0.0613	0.1574	-0.0001	0.3376	0.0064	-0.0264	0.4264	0.0128	0.3457	14.1881	12.6367	66.8839
19	0.0108	0.0144	-0.0097	0.0292	-0.0189	0.0093	-0.0998	-0.1843	0.19	0.1439	-0.0761	-0.6856	0.0596	0.3492	0.0004	0.3919	-0.0115	-0.1044	0.3312	0.0672	0.3762	18.8665	16.5452	73.7311
20	0.0414	0.07	-0.0106	0.1045	-0.0184	0.023	-0.0672	0.2635	0.2879	-0.0839	0.0849	0.3016	-0.0927	-0.0709	0.0004	0.2642	-0.052	0.0087	0.2312	0.0444	0.281	6.3901	25.9111	64.4581
21	1.8056	-0.4134	-0.8325	0.479	0.0116	0.6421	-0.123	0.4743	-0.3019	-0.4097	0	0.3466	0.087	-0.2291	0.0112	0.2322	-0.0727	0.0241	0.2091	0.0548	0.2538	6.1658	32.5428	66.1961
22	0.4853	0.0339	-0.7993	0.0426	-0.1354	1.4858	0.0917	-0.411	-0.0082	1.0755	-0.1663	-0.023	-0.0818	0.3387	-0.0441	0.524	-0.0542	0.0086	0.2207	0.0094	0.291	9.0345	40.0517	79.1613
23	0.2679	-0.1495	-0.227	0.2519	0.101	0.4039	-0.0712	-0.296	0.4809	0.8568	-0.0144	-0.205	-0.4279	0.4327	0.009	0.5692	-0.091	-0.044	0.3404	0.0403	0.3691	16.3506	51.6326	73.4909
24	0.3191	0.1196	-0.0955	0.4274	0.1851	0.1699	0.1516	-0.0366	-0.2219	-0.0558	0.0252	-0.01	0.5351	-0.2342	0.5692	0.6151	0.0149	-0.2088	0.3595	-0.0942	0.4413	-12.4723	-10.9439	15.2453
25	0.1712	-0.2872	0.0278	0.6092	0.3474	0.7779	0.1519	0.0347	-0.1356	-0.0781	0.0072	-0.2483	0.1574	0.221	0.0538	0.3249	0.0317	-0.1112	0.3331	-0.079	0.2977	-6.3151	4.4133	21.7144
26	0.0412	0.0308	0.0328	0.0563	0.0042	0.0179	0.2034	-0.0424	-0.2242	-0.4615	0.0415	-0.2463	0.1225	0.2762	0.0001	0.3038	0.0324	-0.0265	0.496	-0.0991	0.3567	4.8467	20.598	29.0153
27	0.4876	-0.8313	-0.0112	4.2423	0.5415	0.0943	0.0782	0.5626	0.0618	-0.8739	0.1009	0.3487	-0.0662	-0.2445	-0.1894	0.1273	-0.0088	-0.0882	0.2977	-0.0558	0.2499	-11.9298	31.0193	24.8652
28	1.0037	0.6179	-0.2174	0.6836	-0.7017	1.1113	0.0997	-0.4019	-0.0283	-0.0014	-0.1903	0.1267	-0.2336	0.1736	-0.061	0.1788	0.0362	-0.0014	0.3917	-0.1232	0.3855	-8.4376	40.2782	39.2082
29	0.0151	-0.0227	0.0538	0.1292	-0.0483	0.14	0.0368	0.1162	0.2839	-0.2258	-0.1312	0.2161	-0.2525	0.1588	-0.0001	0.2587	0.0395	-0.0339	0.3516	-0.0496	0.3799	-5.9425	53.8046	33.199
30	0.6403	0.0541	-0.0766	0.012	-0.0903	2.2243	0.2003	-0.1203	-0.5178	-0.2304	0.105	0.143	1.191	-0.0467	0.1575	0.7039	0.2209	-0.3223	0.4111	-0.1736	0.6558	4.4633	-15.3242	27.2902
31	0.0394	-0.0896	0.0858	0.4528	-0.4823	0.7293	0.1213	-0.2307	-0.1805	-0.4237	0.2684	0.0124	0.7453	0.325	0.0065	0.5944	0.2433	-0.1706	0.78	-0.2179	0.6262	10.819	-6.5018	25.1528
32	0.7653	0.1966	0.195	0.5815	-0.2953	0.437	0.1738	-0.0594	-0.1097	0.0834	-0.0015	-0.159	0.3903	0.253	0.3012	0.3615	0.0802	-0.1229	0.3629	-0.092	0.3332	-0.29	4.0196	31.6705
33	0.327	-0.2829	0.1053	0.4611	-0.2446	0.1552	0.1071	-0.3855	0.1619	0.4921	0.1614	0.1846	0.059	0.1874	0.0523	0.2796	-0.0316	-0.0763	0.3044	-0.0435	0.3536	-10.9358	19.0014	40.2441
34	0.0023	0.0205	0.0225	0.1021	0.1031	0.1024	0.0272	0.1707	-0.0906	-0.1638	0.1597	-0.2116	-0.0585	0.3311	0.0138	0.2418	0.0182	-0.0751	0.3194	-0.0478	0.2695	2.3102	26.6188	30.5061
35	0.0296	0.1863	-0.0515	1.2639	-0.3794	0.1191	0.099	0.1691	-0.1705	-0.2432	-0.0071	-0.6246	0.2196	0.7026	-0.1434	0.1868	-0.0375	-0.0119	0.329	-0.0887	0.203	1.4643	33.333	29.6029
36	1.6771	0.2479	0.0615	1.2687	0.3971	0.3779	-0.011	-0.1152	0.1934	-0.0546	-0.048	0.6128	0.0427	-0.3795	0.241	0.1117	0.0512	-0.1598	0.2677	-0.0709	0.2981	-11.9226	41.5144	27.7728
37	0.0999	-0.0545	0.0955	0.6422	-0.149	0.115	0.177	-0.3026	0.2671	-0.0447	-0.0385	0.4386	-0.1582	0.0829	0.0204	0.2129	0.0082	0.0013	0.3826	-0.0717	0.3967	-7.0069	53.7133	34.8551
38	1.5537	0.6534	-1.1034	0.295	-0.4897	0.77	-0.1149	0.1416	-0.8242	0.2051	0.0395	-0.428	0.9697	-0.0709	-1.5278	0.9397	-0.0242	-0.3596	0.4001	-0.0517	0.7724	-10.5381	-19.6105	33.3019
39	0.064	-0.1222	-0.0233	0.213	0.0542	0.1541	0.275	-0.1734	-0.3737	0.0935	0.0006	0.2229	0.3932	-0.0838	0.0976	0.5852	-0.089	-0.2213	0.3737	0.0483	0.4448	-12.2971	-8.9385	42.0831
40	0.8516	0.2319	-0.1973	0.0684	0.0007	0.5953	0.2825	-0.0612	-0.3438	0.2678	0.0301	-0.0306	0.623	-0.1131	0.314	0.4507	-0.0028	-0.2048	0.3173	-0.0222	0.3749	-7.9382	-3.187	35.0086
41	0.0085	0.0028	0.002	0.0146	-0.0127	0.01	0.251	-0.1824	-0.0729	0.2758	-0.1718	0.1486	-0.0066	-0.3169	0.0001	0.3928	-0.0004	-0.0471	0.4661	-0.0685	0.3947	-21.2424	6.8178	24.5183
42	0.0701	0.0855	0.0307	0.1065	0.0224	0.036	0.0271	0.0556	0.1697	-0.2346	0.0187	0.209	0.2665	0.1313	-0.0001	0.286	-0.0193	-0.0642	0.3941	-0.0553	0.3423	-15.4699	15.1766	27.4566
43	0.0157	-0.0219	-0.0103	0.0273	-0.0142	0.0339	0.2832	0.0104	-0.0811	-0.1856	0.1192	0.346	-0.0543	-0.0901	0.003	0.3221	0.019	-0.0499	0.3311	-0.1092	0.3801	-12.9935	21.4179	20.9748
44	0.0462	-0.0537	0.0425	0.067	-0.0614	0.0407	0.0329	-0.4012	0.3341	0.2435	0.0288	0.0594	-0.0649	-0.1137	0.0083	0.1449	-0.0446	0.006	0.3218	-0.0688	0.3125	-10.3735	25.3854	38.4171
45	0.1931	0.0106	-0.109	0.2386	-0.055	0.0647	0.2496	-0.5477	-0.0378	0.5753	-0.0504	0.4087	0.1623	0.0723	0.014	0.2672	-0.0281	-0.0235	0.3946	-0.0829	0.4121	-8.6432	31.9082	39.6108
46	0.0139	0.0187	-0.0042	0.0317	-0.0139	0.0302	0.1867	0.1934	-0.0441	-0.1543	-0.2053	-0.1567	-0.2768	0.0903	-0.0002	0.2861	-0.0059	-0.0203	0.4231	-0.0407	0.295	-0.459	41.5052	31.2768
47	0.3087	0.1284	0.2614	0.1737	-0.0476	0.4976	0.0854	0.1067	-0.2195	-0.1951	0.0325	0.2882	-0.0397	-0.2146	-0.1221	0.0666	0.076	-0.0411	0.2549	-0.0933	0.4189	-11.5732	48.4047	35.1105
48	0.0484	0.2762	0.0969	1.5096	0.5291	0.1855	-0.0338	-0.0069	0.3348	-0.4237	0.0774	0.2127	-0.2364	0.0116	0.0456	0.1969	0.0118	-0.018	0.3709	-0.031	0.3075	-5.2745	57.8715	32.0356
49	0.4424	0.0792	-0.1054	0.1833	-0.152	0.4016	0.2392	-0.1264	0.5386	-0.1628	-0.223	-0.3115	-0.7245	0.1652	0.0003	0.626	0.0021	0.041	0.3985	-0.0684	0.5021	11.06	-28.9633	37.907
50	0.2227	-0.2751	0.0632	1.0933	0.2191	0.136	0.0222	-0.0123	0.1124	0.2994	-0.1069	0.0079	-0.242	-0.0505	0	0.4015	-0.0414	0.0288	0.3566	-0.0085	0.3022	10.853	-47.0265	44.8977
51	0.1399	-0.0234	-0.0615	0.0096	0.0026	0.091	0.0727	0.0316	0.0667	0.8085	0.1665	0.6295	-0.2533	0.1515	0.022	0.578	-0.0613	0.0458	0.3097	-0.0292	0.2681	10.2966	-64.1139	56.8222
52	0.0105	-0.0602	-0.0146	0.3089	0.1708	0.1198	-0.0118	0.5774	-0.1327	-0.4082	-0.0473	-0.0108	-0.0581	-0.1701	-0.0006	0.1945	-0.0339	0.0165	0.3928	0.013	0.3341	13.798	-73.4472	39.483
53	0.046	-0.2225	-0.0851	0.9393	0.1613	0.3639	0.0639	-0.0878	-0.0426	0.2803	-0.1339	-0.1297	-0.0433	-0.0098	0.0101	0.1755	-0.0389	-0.0024	0.377	0.0145	0.2735	18.0846	-93.1124	47.2423
54	1.9389	0.0345	-0.5515	0.2945	0.0004	0.0881	0.1746	-0.0083	0.4664	0.5046	-0.0012	-0.11	0.2982	0.1966	0.0265	0.9315	-0.0344	-0.0104	0.4159	-0.1123	0.4568	13.0686	-22.7781	57.2821
55	0.138	-0.1943	0.19	0.6572	-0.4518	0.3462	-0.1412	-0.2948	0.3808	0.9343	-0.2783	0.2072	-0.9972	-0.0713	-0.618	0.8307	-0.0652	0.0702	0.3434	-0.1855	0.2268	7.422	-39.9465	57.5193
56	0.0793	0.3232	0.0332	2.0472	0.3074	0.3169	-0.0528	-0.123	0.0918	0.9472	-0.013	-0.5548	-0.2494	0.2085	-0.3994	0.6262	-0.0754	-0.0895	0.4053	-0.0555	0.31	18.3547	-47.3839	56.4686
57	0.0303	-0.0179	-0.0129	0.0195	-0.002	0.0094	-0.0601	-0.2141	0.0907	0.2955	-0.0754	-0.6075	0.0935	0.1216	-0.0001	0.3641	-0.0969	-0.1425	0.482	0.0398	0.5389	28.0354	-63.9204	48.6757
58	0.1861	-0.0972	-0.0134	0.3432	0.2131	0.1578	0.1248	-0.1809	-0.2681	0.6111	0.001	0.4583	0.0876	-0.3976	0.0235	0.4308	-0.046	0.0294	0.394	-0.0024	0.2771	12.2134	-70.1917	54.9736
59	0.1202	0.189	-0.0961	0.5504	-0.3166	0.1697	-0.018	-0.0299	-0.0026	0.3181	-0.1067	0.0094	-0.1431	-0.0304	-0.012	0.2532	-0.0448	-0.0197	0.3709	0.0144	0.2854	12.3386	-79.5553	48.8591
60	0.0735	0.0229	-0.0854	0.2014	0.1178	0.2055	0.014	-0.4368	-0.4867	0.1986	-0.0836	-0.176	0.2981	0.3066	-0.0013	0.2662	-0.026	-0.0094	0.4485	0.0541	0.3948	17.568	-97.4126	47.6515
61	1.785	0.6366	0.0488	0.9689	-0.5025	2.0651	0.3194	-0.4799	0.7358	0.356	0.0875	-0.473	-0.4965	-0.0879	2.6319	0.5207	-0.2011	-0.2664	0.2307	-0.1131	0.9615	29.5867	-30.7034	49.6811
62	0.7923	-0.4843	0.0219	0.8614	-0.2055	0.3497	0.0548	0.0846	0.1593	0.1028	-0.1633	-0.483	-0.1506	0.0618	-0.0054	0.3668	-0.0996	-0.0152	0.3316	0.0148	0.4041	22.4937	-47.2852	45.1081
63	0.0261	-0.0318	-0.0032	0.0354	0.0054	0.0196	0.0897	-0.0318	0.0356	-0.4672	-0.0425	-0.2096	-0.0427	-0.0856	-0.0001	0.3376	-0.027	0.0301	0.3322	-0.0092	0.3648	13.0916	-63.6857	37.4995
64	-0.002	-0.0674	-0.0378	0.9814	0.1216	0.2607	-0.0067	-0.1395	0.1349	0.272	-0.1496	-0.7564	-0.1545	0.6048	-0.0255	0.2349	-0.0509	-0.0521	0.4034	0.05	0.3305	24.9272	-74.0352	48.7972
65	0.022	-0.0177	-0.022	0.091	-0.0368	0.0508	-0.0301	-0.0289	0.1834	0.2787	-0.1582	0.4252	0.025	-0.0624	0.0002	0.2028	-0.0642	0.0207	0.3326	0.0434	0.2704	12.4163	-85.0048	51.8236
66	0.101	0.0423	0.0792	0.347	0.0984	0.218	0.1543	0.2565	-0.2838	-0.0072	-0.0548	-0.1963	0.2055	0.1093	0.0038	0.2491	-0.012	0.0142	0.3585	0.0106	0.3164	16.905	-97.5155	44.9648
67	0.2277	0.0429	-0.0852	0.0832	0.0027	0.0337	0.0052	0.0463	0.5317	0.1311	0.1243	0.399	-0.6741	0.0883	0.0001	0.7762	0.0339	0.0545	0.3885	0.0276	0.5334	20.1556	-26.6762	14.7841
68	0.7909	-0.7624	0.0727	1.021	-0.0708	0.0138	-0.0474	-0.3007	0.3378	0.8802	0.1591	-0.1644	-0.3908	0.1842	-0.0081	0.5297	0.0766	0.0432	0.3603	0.0399	0.2563	17.7913	-39.4959	19.9477
69	0.1863	-0.2194	-0.2002	0.433	-0.1491	0.2934	-0.1777	-0.0565	-0.0595	0.173	0.1301	-0.0423	-0.0973	0.1291	-0.0054	0.2786	0.0403	-0.012	0.3073	0.044	0.1859	28.3992	-48.2641	11.6281
70	0.3976	0.2197	0.0143	0.1722	-0.1867	0.7106	0.1596	-0.2525	-0.3989	0.3074	-0.0952	-0.4119	0.1406	0.2038	0.014	0.2946	-0.028	0.0386	0.4095	0.0639	0.272	41.8708	-62.2796	4.0828
71	0.0174	-0.0166	-0.0175	0.0115	0.001	0.0624	0.2322	-0.1633	-0.309	0.3001	0.217	0.238	0.1682	-0.2146	0.0006	0.2937	0.0281	-0.0111	0.3488	0.092	0.3126	28.6334	-71.3452	12.5763
72	0.0204	0.0025	-0.0343	0.1366	-0.0645	0.0767	-0.1277	-0.0404	0.1293	0.0175	0.1132	0.8719	0.0243	-0.555	-0.0021	0.3395	0.0275	0.0006	0.4212	0.1042	0.3836	28.495	-78.3277	10.6574
73	0.0459	0.1648	-0.0189	0.596	-0.0563	0.0019	-0.0452	0.3691	0.1333	-0.9128	-0.2289	0.0453	0.1474	0.2609	-0.0785	0.4045	0.1299	-0.0153	0.3558	-0.0121	0.2629	31.9027	-85.7589	-1.6547
74	0	0.0604	-0.0079	0.9391	-0.1549	0.0379	-0.2034	0.0195	-0.0747	-0.8727	-0.0584	-0.1538	0.2911	0.2651	-0.0881	0.4795	0.1663	0.0529	0.3764	0.0256	0.3458	38.1667	-97.1051	4.5921
75	0.1437	-0.0241	0.1068	0.0182	-0.0357	0.2954	-0.0993	-0.0434	0.4766	0.0458	0.0719	-0.2433	-0.769	0.1977	-0.0039	0.5618	-0.0686	0.0119	0.3829	0.0386	0.4406	36.4554	-28.6678	4.2531
76	0.3232	-0.1065	-0.101	0.3697	-0.1104	0.2028	-0.0015	0.0968	0.0746	-0.1568	-0.0909	-0.1152	-0.1504	0.193	0	0.3218	-0.0442	0.0126	0.2645	0.0305	0.2095	33.3438	-42.2854	3.1669
77	0.0544	-0.0305	0.0484	0.0075	0.021	0.157	-0.136	-0.1339	0.0928	-0.5017	0.0591	-0.075	-0.1812	0.1919	0	0.3464	0.0164	0.0214	0.3981	0.0631	0.265	28.9904	-61.2432	-4.8055
78	0.0295	-0.0014	-0.0223	0.0653	0.0029	0.0107	0.1926	-0.1537	0.127	0.0904	0.483	-0.2572	-0.0894	0.1441	0.0038	0.411	0.0163	0.0809	0.4748	0.0112	0.2971	40.7056	-68.7999	3.5047
79	0.0273	0.02	0.0162	0.0141	0.0145	0.0084	0.1119	-0.2892	0.3334	-0.0996	-0.2395	-0.5238	0.1783	0.3404	0	0.3026	0.049	0.0642	0.4098	0.0308	0.3137	41.357	-75.437	5.4894
80	0.7947	0.0668	-0.433	0.0817	-0.0246	0.2386	0.1831	-0.154	0.029	0.1221	0.098	0.1585	0.1711	0.0623	0.11	0.26	0.0593	-0.0331	0.298	0.1067	0.2865	30.7012	-86.0545	9.6245
81	0.0801	0.0808	-0.0101	0.1544	0.0627	0.082	0.0662	0.1054	-0.3335	-0.6835	-0.0846	0.1808	0.4995	0.3815	-0.0001	0.6431	0.1368	0.0241	0.4896	0.0014	0.4921	37.3109	-97.4583	2.4965
82	0.2804	-0.0611	0.1184	0.3013	-0.1553	0.1367	0.0824	0.2209	0.2431	-0.3294	-0.0241	0.1726	-0.5695	-0.0283	0.0126	0.4513	0.0458	0.0096	0.2869	0.0655	0.2802	17.0085	-31.7675	-4.6148
83	0.0652	-0.1168	0.101	0.1374	-0.1196	0.0957	-0.0396	0.0547	-0.0703	-0.2472	0.0358	0.0401	0.0379	-0.1456	0	0.2767	-0.0246	-0.016	0.2767	0.0745	0.2132	24.7113	-46.7811	-2.5286
84	0.428	-0.2353	-0.0512	0.1765	0.1502	0.2458	-0.0815	-0.0502	0.0461	0.0395	0.2613	0.2378	-0.1439	-0.3439	0	0.3165	-0.0027	-0.0221	0.2629	0.0896	0.2476	18.8802	-42.6641	2.8679
85	0.0008	0.007	0.0142	0.1078	0.0159	0.01	-0.1212	-0.1387	-0.2429	0.1151	0.1681	0.3119	0.0958	0.1929	0.0001	0.2939	0.0417	-0.0034	0.3642	0.0856	0.3174	21.6568	-61.139	8.1389
86	0.0049	0.0083	0.0055	0.0341	-0.0261	0.0365	-0.0408	-0.2493	-0.248	0.2046	-0.0374	0.4163	-0.0028	-0.4241	0.0001	0.3648	0.0043	0.023	0.353	0.1192	0.3346	25.8006	-65.6672	14.5217
87	0.081	0.0991	0.0527	0.131	0.0602	0.0316	0.1253	0.1185	-0.2834	-0.5446	-0.0317	-0.1049	0.2297	0.3008	0.0035	0.3441	0.0529	-0.0211	0.337	0.0742	0.2855	26.2895	-71.3475	-2.619
88	0.6362	-1.2711	0.3624	2.6225	-0.8109	0.3818	-0.0918	0.4786	-0.1225	-1.0407	0.1813	0.5876	-0.0266	-0.1605	0.1199	0.4397	0.0478	-0.028	0.3883	-0.0256	0.2557	30.2496	-77.5195	-3.3081
89	0.3496	0.1978	0.155	0.112	0.0815	0.1971	0.1525	-0.1745	0.057	-0.2102	-0.0867	0.0131	0.0983	-0.0152	0.0218	0.3079	0.121	0.1356	0.2831	0.0682	0.3016	41.6357	-83.7668	5.1773
90	0.0746	0.046	0.0125	0.1686	0.0429	0.0377	0.0144	0.0931	-0.2658	-0.3888	-0.209	0.1428	0.3469	0.0575	0.0001	0.486	0.1299	0.0258	0.3585	0.0425	0.4511	36.1923	-97.4842	4.6078
91	0.0375	0.0245	0.134	0.0168	0.0708	0.3522	0.3112	0.022	0.1924	0.2355	0.1253	0.3258	-0.8553	-0.3529	0.1935	1.0387	0.3106	0.7826	0.6581	0.2751	1.3969	-31.7931	34.9798	100.9367
92	0.539	0.3783	-0.341	0.8324	-0.487	0.3974	0.3867	-0.2907	0.8701	0.8899	0.1034	0.547	-0.9316	-0.1956	0.5785	0.9997	0.1258	0.6292	0.3887	0.2583	0.9224	-25.3713	24.2731	103.9417
93	0.0109	-0.0113	-0.02	0.1551	0.0933	0.0732	-0.0578	-0.012	0.3654	0.6212	0.0591	0.4271	-0.7036	0.2745	-0.0121	0.7797	-0.1709	0.2794	0.5086	0.0572	0.6977	-20.5767	9.9069	113.99
94	0.0689	-0.1485	0.0351	0.3254	-0.073	0.0159	0.3146	-0.2919	0.2787	0.4824	0.0028	-0.0778	-0.4606	0.2221	-0.0165	0.7063	-0.2501	0.2094	0.4297	0.0005	0.5393	-13.0265	5.4316	116.5147
95	0.079	-0.0657	0.0801	0.1314	-0.125	0.1141	0.1637	0.2998	0.216	-0.2118	0.2108	0.8262	-0.2748	-0.351	0.0298	0.3793	-0.0149	0.0398	0.443	0.102	0.5233	-25.3723	-6.8354	106.3966
96	0.6825	-0.9882	-0.128	1.9822	0.6686	0.4457	0.0015	-0.0113	0.0512	-0.4061	-0.3836	-0.4508	-0.0977	0.0945	-0.1659	0.2454	-0.0238	0.0537	0.4376	0.1605	0.5372	-12.4598	-17.8598	104.88
97	2.0614	-0.6351	-0.4323	0.3833	-0.1903	1.3982	-0.2998	-0.56	-0.4734	0.089	-0.139	-0.6954	0.4081	0.5727	-0.3619	0.3165	0.0266	0.0774	0.4746	0.2404	0.6765	-16.3852	-29.0808	113.0286
98	0.1654	0.3118	0.1245	1.527	-0.3763	0.6394	0.1621	-0.1654	0.3144	0.6248	0.1294	0.0989	0.2442	-0.1158	0.958	1.6539	-0.232	0.6799	0.59	-0.1446	1.0009	-12.368	37.0575	113.1737
99	0.5859	0.3612	0.2919	0.4676	-0.0095	0.2469	0.3245	-0.3512	0.2802	0.4275	-0.1037	0.3142	-0.6033	0.0939	0.3434	0.8496	-0.1418	0.3285	0.4801	-0.0278	0.6278	-12.3351	20.4443	107.1589
100	0.0825	-0.1039	-0.0307	0.133	-0.0181	0.3457	0.1783	0.0746	0.1168	-0.0178	-0.0914	-0.1666	0.0135	0.0227	0.7212	0.3337	-0.0732	0.1266	0.2902	0.1364	0.3216	-10.8544	2.0176	97.0496
101	0.0299	0.0523	0.0077	0.0744	0.0007	-0	0.1238	-0.9111	-0.2796	0.683	-0.0902	-0.0925	-0.645	0.2472	-0.0001	0.4719	-0.1017	-0.0079	0.624	0.1302	0.5648	-14.1677	-4.9357	115.73
102	0.0734	-0.2705	0.0234	1.1532	-0.2196	0.1558	-0.1646	0.4492	0.2308	-0.5177	-0.0785	0.1548	-0.125	-0.0286	0.0147	0.2511	-0.0535	0.0284	0.533	0.0948	0.5298	-23.1269	-17.575	108.7598
103	0.291	-0.0179	-0.0555	0.3607	-0.3493	0.3714	0.0451	-0.0231	-0.1732	-0.222	-0.1987	-0.3512	0.1234	0.536	-0.0212	0.2636	0.0346	0.0737	0.4942	0.2016	0.6114	-15.1895	-29.1988	111.5113
104	0.0146	0.0026	-0.0166	0.0158	0.0012	0.0133	-0.3328	0.0193	0.1875	0.0944	-0.1353	0.0101	-0.0256	0.1312	-0.0002	1.3001	-0.0789	0.4089	0.9421	0.2299	1.857	-15.8852	48.5791	94.2753
105	0.1503	0.2221	-0.0695	0.6606	0.3333	0.6219	0.3061	-0.0058	0.5307	0.103	0.0224	0.5336	-0.903	-0.271	-0.2676	0.807	0.0468	0.3364	0.3939	0.1484	0.7618	-10.0574	28.8654	89.2135
106	0.3707	-0.0335	-0.1572	0.6296	-0.313	0.327	0.3209	-0.1078	0.7244	0.3004	0.1724	0.519	-0.7961	-0.1825	0.9824	0.7378	-0.0564	0.3887	0.3675	0.1547	0.7204	-15.6887	23.4392	96.457
107	0.0316	-0.0601	0.0046	0.1283	-0.0269	0.0103	0.1489	-0.0037	0.3643	-0.1244	0.1923	0.7425	-0.0033	-0.3228	0.0325	0.549	0.0263	0.1728	0.5209	0.1932	0.6511	-23.9288	7.5174	98.805
108	0.3789	0.4665	-0.2402	0.7759	-0.2743	0.1575	0.0073	-0.1699	0.0579	0.0031	0.1948	0.1745	-0.2914	0.126	0.0357	0.2497	-0.0482	0.1089	0.3808	0.1171	0.3483	-21.2213	0.0599	102.7977
109	0.111	-0.2344	0.1508	1.3671	-0.1088	0.2518	0.0909	-0.5829	0.0182	-0.2368	-0.1829	-0.4356	-0.0219	0.3531	-0.1616	0.2395	-0.0579	0.0238	0.5084	0.1586	0.4708	-12.5474	-12.2863	108.2038
110	0.0765	-0.3211	0.0485	1.6393	-0.1317	0.0909	-0.0847	-0.2834	-0.4725	-0.3861	0.0372	-0.2387	0.2227	-0.0633	-0.0743	0.3097	-0.0184	0.0654	0.5204	0.1831	0.6661	-17.4116	-29.7338	111.0588
111	-0.0012	0.0098	-0.0008	0.0114	-0.0018	0.0017	-0.2701	0.0072	0.21	0.0942	-0.2357	-0.1302	0.2208	-0.2365	0	2.3043	0.7289	0.3632	1.2592	0.2122	1.7463	-41.2703	-11.581	31.6767
112	0.1745	0.0749	0.1744	0.0322	0.073	0.1809	0.2483	0.0701	-0.3125	0.1383	0.2169	-0.2267	0.1982	0.031	0.1077	2.4528	1.1506	0.6232	1.4356	0.5134	2.0572	-36.6049	-7.2839	27.3684
113	0.893	0.3044	0.4898	0.116	0.15	0.2749	-0.059	-0.1819	-0.6378	0.5588	0.1259	-0.3894	0.8725	0.3655	-0.4762	1.755	1.2941	0.6562	0.2511	0.5618	1.3609	-47.2067	3.1167	33.8757
114	0.0331	-0.0271	-0.0308	0.0186	0.0187	0.0232	0.1967	-0.469	-0.1568	0.357	0.0592	0.1311	0.496	-0.1983	0	1.1092	0.2188	0.2364	0.7574	0.2718	0.9822	-57.2778	19.2541	42.3107
115	0.2655	0.0619	0.2577	1.0011	-0.1034	0.2874	-0.1457	-0.2145	0.224	-0.1237	0.0724	-0.8944	0.2679	0.5747	0.1295	0.4364	0.321	0.0522	0.6327	0.1072	0.4805	-46.8306	29.8999	29.0695
116	0.2778	0.235	-0.4991	0.2905	-0.6705	1.5739	-0.1658	0.5274	-0.0892	-0.1886	-0.2434	0.0825	0.2568	-0.191	-0.0372	0.2164	0.0881	0.0003	0.4391	0.0595	0.3153	-59.8771	40.2836	27.0732
117	0.6976	0.5466	0.759	1.8249	1.2248	1.1147	0.0672	-0.1593	0.4335	-0.2612	0.0952	-0.2799	-0.2452	0.3193	0.3326	0.1793	0.0235	0.028	0.5113	0.0224	0.4084	-55.2981	52.9224	32.5191
118	0.013	-0.0241	0.0175	0.0347	-0.0264	0.0178	-0.0544	0.0289	0.0336	0.3516	-0.2622	0.0145	-0.2309	0.2234	0.0003	1.8936	-0.0107	0.5576	1.4474	0.1329	1.2757	-58.7958	-11.4166	41.4893
119	0.1429	-0.0846	0.1398	0.0499	-0.0826	0.1324	-0.8734	-0.1001	0.121	0.1259	-0.5107	-0.2477	-0.0056	-0.0477	-0.0146	2.3781	0.5084	0.4742	1.117	0.1646	1.6077	-56.9352	-8.3141	47.7832
120	0.6741	0.2238	-0.192	0.4315	-0.0162	0.3694	-0.3843	-0.0629	-0.1501	0.6062	0.0992	0.0558	0.6794	-0.0006	-0.5488	1.9411	0.6136	0.4923	0.7642	0.5912	1.3601	-53.7139	1.0369	39.5081
121	-0.0009	0.0025	0.0028	0.008	0.0056	0.0076	0.1491	-0.2203	-0.7307	0.0402	-0.1045	0.6335	1.2933	0.2665	-0.4287	1.5983	0.2487	0.3582	0.626	0.2109	1.1327	-61.2736	3.3745	33.5145
122	0.2124	-0.0251	0.2781	0.1145	-0.1822	0.752	-0.0894	-0.068	-0.2717	-0.457	0.0621	0.257	0.2169	-0.0879	-0.008	0.9592	0.184	0.1946	0.372	-0.0412	0.8877	-61.9215	19.0198	23.4389
123	0.0726	-0.0796	-0.086	1.9959	0.9848	0.5273	-0.3364	-0.6096	-0.4157	1.3126	0.1802	0.1027	0.6883	0.5024	-0.2177	0.5651	0.1663	0.0656	0.6134	0.2287	0.6072	-56.451	29.463	39.6627
124	0.0294	0.2121	0.0237	1.6587	0.2181	0.0299	-0.0204	0.2665	0.04	-0.2884	0.0694	-0.3568	0.0181	0.1836	-0.1047	0.2044	0.1194	0.0473	0.5236	0.0915	0.3654	-48.7588	40.7108	28.7895
125	0.135	0.3682	0.1485	1.6183	1.2288	1.2682	0.0195	-0.2849	0.2207	-0.278	0.1929	-0.1194	-0.3616	0.2469	0.176	0.189	-0.0141	0.0242	0.4808	0.0487	0.3074	-56.6084	53.2308	31.0664
126	0.0223	0.0183	-0.0223	0.1293	0.001	0.3882	0.2555	-0.0166	-0.2442	0.2219	0.1686	0.1443	0.0509	-0.0734	0.1643	1.8106	0.2162	0.5963	0.6391	0.0945	2.3058	-57.6388	-14.9633	21.8606
127	0.3701	-0.5559	-0.1015	0.9375	0.1821	0.0378	-0.003	0.0619	-0.0428	-0.1804	-0.2068	-0.2005	0.2239	0.1416	-0.108	2.4102	0.313	0.6962	0.6383	-0.0365	2.0572	-64.2846	-8.9074	19.6444
128	0.6229	-0.2146	0.6827	0.0883	-0.2406	0.7607	0.2878	0.2545	-0.9519	-0.0866	-0.3275	-0.0524	1.4472	0.3097	0.023	1.7918	0.285	0.4189	0.6682	0.0592	1.3992	-56.4858	-1.53	25.4026
129	0.0437	0.0439	-0.2293	0.0998	-0.3862	1.6533	-0.149	-0.1389	-0.2412	-0.3693	-0.0641	-0.0204	0.3466	-0.2382	-0.0572	0.902	0.3405	0.1817	0.5807	0.025	0.8536	-48.5268	14.47	23.8951
130	0.5171	0.319	0.0624	0.222	0.1097	0.1984	-0.0534	-0.1781	-0.1423	0.1288	0.1471	-0.5421	0.1819	0.3003	0.2982	0.66	0.9751	0.1045	0.9487	0.1531	0.869	-43.2618	20.0604	29.0366
131	0.0206	-0.0243	0.0108	0.0423	0.0133	0.0302	0.3016	-0.1191	0.0377	-0.3002	-0.1171	0.6414	0.1047	-0.2159	-0.0001	0.5631	0.0515	0.0607	0.4701	-0.046	0.5491	-60.1035	25.0621	25.2666
132	0.0043	-0.0215	0.0065	0.0931	-0.0343	0.0137	-0.0667	0.2668	-0.2975	-0.2405	-0.0593	0.6196	0.3705	-0.1453	0.0004	0.3489	0.0916	0.0411	0.358	0.0433	0.5202	-61.3513	31.8235	26.6168
133	0.1013	-0.219	-0.1291	0.4687	0.2813	0.1711	-0.161	-0.4343	-0.1017	0.414	0.1067	-0.2243	0.1476	0.4153	0.0372	0.2629	0.0875	0.0086	0.7073	0.0621	0.4296	-56.6128	40.5499	37.988
134	0.9345	-0.0581	0.4241	0.1635	0.0951	0.3229	0.1819	-0.3261	0.2084	-0.2094	0.0615	-0.4009	-0.2349	0.8319	0.1709	0.2667	0.0118	0.0857	0.6085	0.0203	0.4135	-54.5612	53.1981	30.3746

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	( CHAIN A AND RESID 9:44 )	A	9 - 44
2	X-RAY DIFFRACTION	2	( CHAIN A AND RESID 45:95 )	A	45 - 95
3	X-RAY DIFFRACTION	3	( CHAIN A AND RESID 96:111 )	A	96 - 111
4	X-RAY DIFFRACTION	4	( CHAIN A AND RESID 112:120 )	A	112 - 120
5	X-RAY DIFFRACTION	5	( CHAIN A AND RESID 121:130 )	A	121 - 130
6	X-RAY DIFFRACTION	6	( CHAIN A AND RESID 131:145 )	A	131 - 145
7	X-RAY DIFFRACTION	7	( CHAIN A AND RESID 146:155 )	A	146 - 155
8	X-RAY DIFFRACTION	8	( CHAIN A AND RESID 156:165 )	A	156 - 165
9	X-RAY DIFFRACTION	9	( CHAIN A AND RESID 166:176 )	A	166 - 176
10	X-RAY DIFFRACTION	10	( CHAIN B AND RESID 4:44 )	B	4 - 44
11	X-RAY DIFFRACTION	11	( CHAIN B AND RESID 45:111 )	B	45 - 111
12	X-RAY DIFFRACTION	12	( CHAIN B AND RESID 112:120 )	B	112 - 120
13	X-RAY DIFFRACTION	13	( CHAIN B AND RESID 121:145 )	B	121 - 145
14	X-RAY DIFFRACTION	14	( CHAIN B AND RESID 146:177 )	B	146 - 177
15	X-RAY DIFFRACTION	15	( CHAIN C AND RESID 6:25 )	C	6 - 25
16	X-RAY DIFFRACTION	16	( CHAIN C AND RESID 26:44 )	C	26 - 44
17	X-RAY DIFFRACTION	17	( CHAIN C AND RESID 45:95 )	C	45 - 95
18	X-RAY DIFFRACTION	18	( CHAIN C AND RESID 96:111 )	C	96 - 111
19	X-RAY DIFFRACTION	19	( CHAIN C AND RESID 112:120 )	C	112 - 120
20	X-RAY DIFFRACTION	20	( CHAIN C AND RESID 121:130 )	C	121 - 130
21	X-RAY DIFFRACTION	21	( CHAIN C AND RESID 131:145 )	C	131 - 145
22	X-RAY DIFFRACTION	22	( CHAIN C AND RESID 146:155 )	C	146 - 155
23	X-RAY DIFFRACTION	23	( CHAIN C AND RESID 156:177 )	C	156 - 177
24	X-RAY DIFFRACTION	24	( CHAIN D AND RESID 4:44 )	D	4 - 44
25	X-RAY DIFFRACTION	25	( CHAIN D AND RESID 45:111 )	D	45 - 111
26	X-RAY DIFFRACTION	26	( CHAIN D AND RESID 112:120 )	D	112 - 120
27	X-RAY DIFFRACTION	27	( CHAIN D AND RESID 121:145 )	D	121 - 145
28	X-RAY DIFFRACTION	28	( CHAIN D AND RESID 146:155 )	D	146 - 155
29	X-RAY DIFFRACTION	29	( CHAIN D AND RESID 156:176 )	D	156 - 176
30	X-RAY DIFFRACTION	30	( CHAIN E AND RESID 6:25 )	E	6 - 25
31	X-RAY DIFFRACTION	31	( CHAIN E AND RESID 26:44 )	E	26 - 44
32	X-RAY DIFFRACTION	32	( CHAIN E AND RESID 45:111 )	E	45 - 111
33	X-RAY DIFFRACTION	33	( CHAIN E AND RESID 112:120 )	E	112 - 120
34	X-RAY DIFFRACTION	34	( CHAIN E AND RESID 121:130 )	E	121 - 130
35	X-RAY DIFFRACTION	35	( CHAIN E AND RESID 131:145 )	E	131 - 145
36	X-RAY DIFFRACTION	36	( CHAIN E AND RESID 146:155 )	E	146 - 155
37	X-RAY DIFFRACTION	37	( CHAIN E AND RESID 156:177 )	E	156 - 177
38	X-RAY DIFFRACTION	38	( CHAIN F AND RESID 2:25 )	F	2 - 25
39	X-RAY DIFFRACTION	39	( CHAIN F AND RESID 26:44 )	F	26 - 44
40	X-RAY DIFFRACTION	40	( CHAIN F AND RESID 45:85 )	F	45 - 85
41	X-RAY DIFFRACTION	41	( CHAIN F AND RESID 86:95 )	F	86 - 95
42	X-RAY DIFFRACTION	42	( CHAIN F AND RESID 96:111 )	F	96 - 111
43	X-RAY DIFFRACTION	43	( CHAIN F AND RESID 112:120 )	F	112 - 120
44	X-RAY DIFFRACTION	44	( CHAIN F AND RESID 121:130 )	F	121 - 130
45	X-RAY DIFFRACTION	45	( CHAIN F AND RESID 131:145 )	F	131 - 145
46	X-RAY DIFFRACTION	46	( CHAIN F AND RESID 146:155 )	F	146 - 155
47	X-RAY DIFFRACTION	47	( CHAIN F AND RESID 156:165 )	F	156 - 165
48	X-RAY DIFFRACTION	48	( CHAIN F AND RESID 166:176 )	F	166 - 176
49	X-RAY DIFFRACTION	49	( CHAIN G AND RESID 2:44 )	G	2 - 44
50	X-RAY DIFFRACTION	50	( CHAIN G AND RESID 45:111 )	G	45 - 111
51	X-RAY DIFFRACTION	51	( CHAIN G AND RESID 112:120 )	G	112 - 120
52	X-RAY DIFFRACTION	52	( CHAIN G AND RESID 121:145 )	G	121 - 145
53	X-RAY DIFFRACTION	53	( CHAIN G AND RESID 146:176 )	G	146 - 176
54	X-RAY DIFFRACTION	54	( CHAIN H AND RESID 2:25 )	H	2 - 25
55	X-RAY DIFFRACTION	55	( CHAIN H AND RESID 26:54 )	H	26 - 54
56	X-RAY DIFFRACTION	56	( CHAIN H AND RESID 55:111 )	H	55 - 111
57	X-RAY DIFFRACTION	57	( CHAIN H AND RESID 112:120 )	H	112 - 120
58	X-RAY DIFFRACTION	58	( CHAIN H AND RESID 121:130 )	H	121 - 130
59	X-RAY DIFFRACTION	59	( CHAIN H AND RESID 131:155 )	H	131 - 155
60	X-RAY DIFFRACTION	60	( CHAIN H AND RESID 156:178 )	H	156 - 178
61	X-RAY DIFFRACTION	61	( CHAIN I AND RESID 3:44 )	I	3 - 44
62	X-RAY DIFFRACTION	62	( CHAIN I AND RESID 45:111 )	I	45 - 111
63	X-RAY DIFFRACTION	63	( CHAIN I AND RESID 112:120 )	I	112 - 120
64	X-RAY DIFFRACTION	64	( CHAIN I AND RESID 121:145 )	I	121 - 145
65	X-RAY DIFFRACTION	65	( CHAIN I AND RESID 146:155 )	I	146 - 155
66	X-RAY DIFFRACTION	66	( CHAIN I AND RESID 156:177 )	I	156 - 177
67	X-RAY DIFFRACTION	67	( CHAIN J AND RESID 2:25 )	J	2 - 25
68	X-RAY DIFFRACTION	68	( CHAIN J AND RESID 26:44 )	J	26 - 44
69	X-RAY DIFFRACTION	69	( CHAIN J AND RESID 45:111 )	J	45 - 111
70	X-RAY DIFFRACTION	70	( CHAIN J AND RESID 112:120 )	J	112 - 120
71	X-RAY DIFFRACTION	71	( CHAIN J AND RESID 121:130 )	J	121 - 130
72	X-RAY DIFFRACTION	72	( CHAIN J AND RESID 131:145 )	J	131 - 145
73	X-RAY DIFFRACTION	73	( CHAIN J AND RESID 146:155 )	J	146 - 155
74	X-RAY DIFFRACTION	74	( CHAIN J AND RESID 156:176 )	J	156 - 176
75	X-RAY DIFFRACTION	75	( CHAIN K AND RESID 2:44 )	K	2 - 44
76	X-RAY DIFFRACTION	76	( CHAIN K AND RESID 45:95 )	K	45 - 95
77	X-RAY DIFFRACTION	77	( CHAIN K AND RESID 96:120 )	K	96 - 120
78	X-RAY DIFFRACTION	78	( CHAIN K AND RESID 121:130 )	K	121 - 130
79	X-RAY DIFFRACTION	79	( CHAIN K AND RESID 131:145 )	K	131 - 145
80	X-RAY DIFFRACTION	80	( CHAIN K AND RESID 146:155 )	K	146 - 155
81	X-RAY DIFFRACTION	81	( CHAIN K AND RESID 156:177 )	K	156 - 177
82	X-RAY DIFFRACTION	82	( CHAIN L AND RESID 2:44 )	L	2 - 44
83	X-RAY DIFFRACTION	83	( CHAIN L AND RESID 45:68 )	L	45 - 68
84	X-RAY DIFFRACTION	84	( CHAIN L AND RESID 69:95 )	L	69 - 95
85	X-RAY DIFFRACTION	85	( CHAIN L AND RESID 96:111 )	L	96 - 111
86	X-RAY DIFFRACTION	86	( CHAIN L AND RESID 112:120 )	L	112 - 120
87	X-RAY DIFFRACTION	87	( CHAIN L AND RESID 121:130 )	L	121 - 130
88	X-RAY DIFFRACTION	88	( CHAIN L AND RESID 131:145 )	L	131 - 145
89	X-RAY DIFFRACTION	89	( CHAIN L AND RESID 146:155 )	L	146 - 155
90	X-RAY DIFFRACTION	90	( CHAIN L AND RESID 156:176 )	L	156 - 176
91	X-RAY DIFFRACTION	91	( CHAIN M AND RESID 5:44 )	M	5 - 44
92	X-RAY DIFFRACTION	92	( CHAIN M AND RESID 45:95 )	M	45 - 95
93	X-RAY DIFFRACTION	93	( CHAIN M AND RESID 96:111 )	M	96 - 111
94	X-RAY DIFFRACTION	94	( CHAIN M AND RESID 112:120 )	M	112 - 120
95	X-RAY DIFFRACTION	95	( CHAIN M AND RESID 121:145 )	M	121 - 145
96	X-RAY DIFFRACTION	96	( CHAIN M AND RESID 146:155 )	M	146 - 155
97	X-RAY DIFFRACTION	97	( CHAIN M AND RESID 156:176 )	M	156 - 176
98	X-RAY DIFFRACTION	98	( CHAIN N AND RESID 6:44 )	N	6 - 44
99	X-RAY DIFFRACTION	99	( CHAIN N AND RESID 45:111 )	N	45 - 111
100	X-RAY DIFFRACTION	100	( CHAIN N AND RESID 112:120 )	N	112 - 120
101	X-RAY DIFFRACTION	101	( CHAIN N AND RESID 121:145 )	N	121 - 145
102	X-RAY DIFFRACTION	102	( CHAIN N AND RESID 146:155 )	N	146 - 155
103	X-RAY DIFFRACTION	103	( CHAIN N AND RESID 156:176 )	N	156 - 176
104	X-RAY DIFFRACTION	104	( CHAIN O AND RESID 4:15 )	O	4 - 15
105	X-RAY DIFFRACTION	105	( CHAIN O AND RESID 16:44 )	O	16 - 44
106	X-RAY DIFFRACTION	106	( CHAIN O AND RESID 45:95 )	O	45 - 95
107	X-RAY DIFFRACTION	107	( CHAIN O AND RESID 96:111 )	O	96 - 111
108	X-RAY DIFFRACTION	108	( CHAIN O AND RESID 112:130 )	O	112 - 130
109	X-RAY DIFFRACTION	109	( CHAIN O AND RESID 131:155 )	O	131 - 155
110	X-RAY DIFFRACTION	110	( CHAIN O AND RESID 156:177 )	O	156 - 177
111	X-RAY DIFFRACTION	111	( CHAIN P AND RESID 11:25 )	P	11 - 25
112	X-RAY DIFFRACTION	112	( CHAIN P AND RESID 26:44 )	P	26 - 44
113	X-RAY DIFFRACTION	113	( CHAIN P AND RESID 45:111 )	P	45 - 111
114	X-RAY DIFFRACTION	114	( CHAIN P AND RESID 112:120 )	P	112 - 120
115	X-RAY DIFFRACTION	115	( CHAIN P AND RESID 121:145 )	P	121 - 145
116	X-RAY DIFFRACTION	116	( CHAIN P AND RESID 146:155 )	P	146 - 155
117	X-RAY DIFFRACTION	117	( CHAIN P AND RESID 156:176 )	P	156 - 176
118	X-RAY DIFFRACTION	118	( CHAIN Q AND RESID 12:25 )	Q	12 - 25
119	X-RAY DIFFRACTION	119	( CHAIN Q AND RESID 26:44 )	Q	26 - 44
120	X-RAY DIFFRACTION	120	( CHAIN Q AND RESID 45:68 )	Q	45 - 68
121	X-RAY DIFFRACTION	121	( CHAIN Q AND RESID 69:111 )	Q	69 - 111
122	X-RAY DIFFRACTION	122	( CHAIN Q AND RESID 112:120 )	Q	112 - 120
123	X-RAY DIFFRACTION	123	( CHAIN Q AND RESID 121:145 )	Q	121 - 145
124	X-RAY DIFFRACTION	124	( CHAIN Q AND RESID 146:155 )	Q	146 - 155
125	X-RAY DIFFRACTION	125	( CHAIN Q AND RESID 156:177 )	Q	156 - 177
126	X-RAY DIFFRACTION	126	( CHAIN R AND RESID 9:25 )	R	9 - 25
127	X-RAY DIFFRACTION	127	( CHAIN R AND RESID 26:44 )	R	26 - 44
128	X-RAY DIFFRACTION	128	( CHAIN R AND RESID 45:95 )	R	45 - 95
129	X-RAY DIFFRACTION	129	( CHAIN R AND RESID 96:111 )	R	96 - 111
130	X-RAY DIFFRACTION	130	( CHAIN R AND RESID 112:120 )	R	112 - 120
131	X-RAY DIFFRACTION	131	( CHAIN R AND RESID 121:130 )	R	121 - 130
132	X-RAY DIFFRACTION	132	( CHAIN R AND RESID 131:145 )	R	131 - 145
133	X-RAY DIFFRACTION	133	( CHAIN R AND RESID 146:155 )	R	146 - 155
134	X-RAY DIFFRACTION	134	( CHAIN R AND RESID 156:177 )	R	156 - 177

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