- PDB-4rp3: Crystal Structure of the L27 Domain of Discs Large 1 (target ID N... -
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Open data
ID or keywords:
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Basic information
Entry
Database: PDB / ID: 4rp3
Title
Crystal Structure of the L27 Domain of Discs Large 1 (target ID NYSGRC-010766) from Drosophila melanogaster bound to a potassium ion (space group P212121)
Components
Disks large 1 tumor suppressor protein
Keywords
ANTITUMOR PROTEIN / NYSGRC / Structural Genomics / PSI-Biology / New York Structural Genomics Research Consortium / Scaffolding
Function / homology
Function and homology information
smooth septate junction / positive phototaxis / RHO GTPases activate CIT / Neurexins and neuroligins / establishment or maintenance of polarity of larval imaginal disc epithelium / cell fate commitment involved in pattern specification / type I terminal bouton / septate junction / septate junction assembly / negative regulation of imaginal disc growth ...smooth septate junction / positive phototaxis / RHO GTPases activate CIT / Neurexins and neuroligins / establishment or maintenance of polarity of larval imaginal disc epithelium / cell fate commitment involved in pattern specification / type I terminal bouton / septate junction / septate junction assembly / negative regulation of imaginal disc growth / Activation of Ca-permeable Kainate Receptor / morphogenesis of follicular epithelium / type Ib terminal bouton / subsynaptic reticulum / Synaptic adhesion-like molecules / establishment or maintenance of polarity of follicular epithelium / morphogenesis of larval imaginal disc epithelium / Unblocking of NMDA receptors, glutamate binding and activation / gravitaxis / tricellular tight junction / establishment of spindle orientation / anterior/posterior axis specification, follicular epithelium / negative regulation of peptidoglycan recognition protein signaling pathway / pole plasm protein localization / basal protein localization / asymmetric protein localization involved in cell fate determination / dorsal closure / follicle cell of egg chamber development / regulation of epidermal growth factor receptor signaling pathway / positive regulation of synaptic assembly at neuromuscular junction / male courtship behavior / guanylate kinase activity / morphogenesis of a polarized epithelium / behavioral response to ethanol / receptor localization to synapse / apical cortex / leading edge membrane / apicolateral plasma membrane / synaptic assembly at neuromuscular junction / establishment or maintenance of epithelial cell apical/basal polarity / mating behavior / cell fate specification / receptor clustering / locomotor rhythm / establishment of mitotic spindle orientation / lateral plasma membrane / postsynaptic density membrane / neuromuscular junction / terminal bouton / kinase binding / cell-cell adhesion / nervous system development / cell cortex / chemical synaptic transmission / basolateral plasma membrane / postsynaptic membrane / cytoskeleton / neuron projection / synapse / perinuclear region of cytoplasm / signal transduction / plasma membrane Similarity search - Function
Mass: 18.015 Da / Num. of mol.: 163 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.95 Å3/Da / Density % sol: 36.89 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M Sodium Format, 20 % PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K
Method to determine structure: SAD / Resolution: 1.36→30 Å / Cor.coef. Fo:Fc: 0.976 / Cor.coef. Fo:Fc free: 0.958 / SU B: 2.603 / SU ML: 0.046 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.057 / ESU R Free: 0.061 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.2056
1959
5 %
RANDOM
Rwork
0.1472
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obs
0.1501
37070
99.54 %
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all
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37358
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
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