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- PDB-4o5p: Crystal structure of an uncharacterized protein from Pseudomonas ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4o5p | ||||||
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Title | Crystal structure of an uncharacterized protein from Pseudomonas aeruginosa | ||||||
![]() | Uncharacterized protein | ||||||
![]() | HYDROLASE / phospholipase effector | ||||||
Function / homology | Domain of unknown function DUF2235 / T6SS, Phospholipase effector Tle1-like, catalytic domain / Uncharacterized protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hu, H.D. / Gao, Z.Q. / Zhang, H. / Dong, Y.H. | ||||||
![]() | ![]() Title: Structure of the type VI secretion phospholipase effector Tle1 provides insight into its hydrolysis and membrane targeting. Authors: Hu, H.D. / Zhang, H. / Gao, Z.Q. / Wang, D. / Liu, G. / Xu, J. / Lan, K. / Dong, Y.H. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 320 KB | Display | ![]() |
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PDB format | ![]() | 263 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 435.6 KB | Display | ![]() |
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Full document | ![]() | 452.8 KB | Display | |
Data in XML | ![]() | 60.7 KB | Display | |
Data in CIF | ![]() | 90.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 97574.617 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.35 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.04M citric acid, 0.1M Bis-Tris propane, 17% pEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Sep 25, 2013 |
Radiation | Monochromator: double crystal monochromator / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. all: 109609 / Num. obs: 109609 / % possible obs: 100 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.3 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 24.6 |
Reflection shell | Resolution: 2→2.03 Å / Redundancy: 3.1 % / Rmerge(I) obs: 0.212 / Mean I/σ(I) obs: 4.3 / Num. unique all: 5486 / % possible all: 92.3 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.001→28.298 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30
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