ソフトウェア 名称 バージョン 分類 ADSCQuantumデータ収集 PHASES位相決定 PHENIX(phenix.refine: 1.7_650)精密化 MOSFLMデータ削減 SCALAデータスケーリング
精密化 構造決定の手法 : 分子置換開始モデル : PDB ENTRY 4LJL解像度 : 1.8→33.155 Å / SU ML : 0.22 / σ(F) : 0 / 位相誤差 : 24.72 / 立体化学のターゲット値 : MLRfactor 反射数 %反射 Selection details Rfree 0.2371 1759 5.03 % Random Rwork 0.194 - - - obs 0.1962 34988 80.5 % -
溶媒の処理 減衰半径 : 0.95 Å / VDWプローブ半径 : 1.2 Å / 溶媒モデル : FLAT BULK SOLVENT MODEL / Bsol : 43.5 Å2 / ksol : 0.348 e/Å3 原子変位パラメータ Baniso -1 Baniso -2 Baniso -3 1- -2.7783 Å2 -0 Å2 1.3559 Å2 2- - 7.0078 Å2 0 Å2 3- - - -4.2295 Å2
精密化ステップ サイクル : LAST / 解像度 : 1.8→33.155 Åタンパク質 核酸 リガンド 溶媒 全体 原子数 3318 142 0 385 3845
拘束条件 Refine-ID タイプ Dev ideal 数 X-RAY DIFFRACTION f_bond_d0.004 3530 X-RAY DIFFRACTION f_angle_d0.87 4804 X-RAY DIFFRACTION f_dihedral_angle_d13.933 1360 X-RAY DIFFRACTION f_chiral_restr0.059 564 X-RAY DIFFRACTION f_plane_restr0.004 593
LS精密化 シェル 解像度 (Å)Rfactor Rfree Num. reflection Rfree Rfactor Rwork Num. reflection Rwork Refine-ID % reflection obs (%)1.8-1.8487 0.3342 111 0.2683 2275 X-RAY DIFFRACTION 71 1.8487-1.9031 0.2936 108 0.2564 2098 X-RAY DIFFRACTION 67 1.9031-1.9645 0.4481 111 0.3552 2000 X-RAY DIFFRACTION 64 1.9645-2.0347 0.2753 122 0.2092 2397 X-RAY DIFFRACTION 76 2.0347-2.1162 0.244 132 0.1992 2423 X-RAY DIFFRACTION 77 2.1162-2.2125 0.2382 118 0.1938 2469 X-RAY DIFFRACTION 78 2.2125-2.3291 0.3759 103 0.2712 2044 X-RAY DIFFRACTION 65 2.3291-2.475 0.214 120 0.1896 2647 X-RAY DIFFRACTION 83 2.475-2.666 0.2463 172 0.2006 2831 X-RAY DIFFRACTION 89 2.666-2.9341 0.2589 159 0.1971 2931 X-RAY DIFFRACTION 93 2.9341-3.3583 0.2389 172 0.1857 3049 X-RAY DIFFRACTION 96 3.3583-4.2298 0.1816 175 0.1576 3051 X-RAY DIFFRACTION 95 4.2298-33.1604 0.1985 156 0.1711 3014 X-RAY DIFFRACTION 91
精密化 TLS 手法 : refined / Refine-ID : X-RAY DIFFRACTION
大きな表を表示 (25 x 19) 大きな表を隠す ID L11 (°2 )L12 (°2 )L13 (°2 )L22 (°2 )L23 (°2 )L33 (°2 )S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2 )T12 (Å2 )T13 (Å2 )T22 (Å2 )T23 (Å2 )T33 (Å2 )Origin x (Å)Origin y (Å)Origin z (Å)1 0.4476 0.256 0.0526 0.427 -0.1123 0.3704 0.0444 -0.0082 -0.1419 -0.0068 -0.1342 -0.1252 0.0965 -0.1158 0.0011 0.1151 0.0099 -0.0037 0.1092 -0.036 0.167 18.7643 -8.0199 2.4677 2 0.1479 -0.0931 -0.3887 0.2112 0.1736 1.1938 0.0043 0.1941 -0.0812 -0.0862 -0.1223 -0.0131 0.1404 -0.4792 0.0063 0.1211 -0.0099 0.0088 0.0936 -0.0324 0.1926 7.3728 -5.8618 6.2797 3 0.7097 0.0299 0.2955 0.3773 0.2786 0.3264 -0.0893 -0.6978 -0.0024 0.2098 -0.0133 0.1053 0.116 0.1369 -0.0048 0.2094 -0.0146 -0.0344 0.398 0.0103 0.1409 16.3744 -4.663 27.4294 4 0.2958 0.0549 0.0752 0.1317 0.0095 0.2141 -0.0189 -0.2066 0.0619 -0.0378 -0.0736 -0.0457 0.0647 0.0468 -0.0263 0.0906 0.0011 -0.0167 0.1041 -0.0141 0.1115 19.3208 -4.8264 16.0887 5 0.7367 -0.2925 -0.3939 0.4393 -0.3388 1.0068 -0.1609 -0.2248 -0.3476 -0.1202 -0.0188 -0.198 0.359 0.4254 0.0759 0.1213 0.0186 -0.0204 0.2102 0.0331 0.2172 26.7858 -7.8129 14.1504 6 0.087 -0.1424 0.0077 0.2815 0.0112 0.0921 -0.0658 0.194 -0.022 -0.2177 -0.0668 -0.1236 0.0659 0.238 -0.0342 0.1036 0.0526 -0.0047 -0.3573 0.0312 0.224 18.5746 0.363 3.6128 7 0.2114 -0.0234 0.3153 0.4545 0.1567 0.636 -0.0005 -0.4587 0.1757 -0.0299 -0.116 0.0018 -0.0474 -0.4865 0.0455 0.1294 0.0481 -0.0246 0.1022 -0.1042 0.2003 11.4373 4.4069 18.0184 8 0.3545 0.1959 0.2314 0.4141 -0.0019 0.2988 0.0636 -0.3261 0.2029 -0.1299 -0.0738 0.1977 -0.0571 0.1189 0.0146 0.1985 0.0296 -0.0478 0.2954 -0.1154 0.2042 4.6217 4.7229 22.3252 9 0.1221 -0.0221 -0.004 0.0346 0.0063 0.0301 -0.0056 0.035 0.1004 -0.0688 -0.0113 0.0177 -0.1571 -0.0332 0.0567 0.2877 0.1202 0.0187 0.605 -0.5059 -0.1622 11.5667 9.8239 34.7415 10 0.6456 0.3225 0.3893 0.9255 0.2596 0.4967 0.0115 -0.8087 -0.1287 0.4731 -0.1987 0.1387 0.2051 -0.333 0.003 0.2051 -0.0426 0.1071 0.5807 0.0844 0.1792 4.5931 -5.695 32.798 11 0.7953 -0.1343 -0.0152 0.3993 -0.0197 0.4034 0.0297 -0.0926 0.1125 0.2209 -0.091 0.1439 -0.062 -0.0539 0.0288 0.2242 -0.0048 0.0305 0.1443 -0.0467 0.1858 5.3099 17.7433 77.1581 12 0.1951 0.0998 -0.232 1.0093 -0.0853 0.723 0.0989 0.1772 0.0155 0.1206 -0.1505 0.4703 -0.0681 -0.4823 0.0604 0.1324 0.015 0.0379 0.2669 -0.0611 0.2947 -3.7325 15.7987 69.4853 13 0.4095 0.2265 0.1359 0.3207 0.2321 0.1732 -0.1167 0.4963 0.072 -0.1565 0.1528 -0.089 -0.2028 0.126 -0.0379 0.2196 0.0571 0.0294 0.2863 -0.0229 0.1026 11.9252 15.589 52.8251 14 0.6048 -0.2974 -0.135 0.7493 0.3205 0.7702 -0.0095 0.065 -0.0873 0.0422 -0.0431 0.0604 0.0095 0.0018 0.0156 0.1634 0.0019 0.0249 0.1416 -0.0255 0.1552 9.3669 13.0311 67.4689 15 0.5237 -0.0788 0.2538 0.3699 -0.3235 0.3705 -0.2338 0.3464 -0.2324 0.0818 -0.2273 0.2848 0.1395 -0.4121 0.0768 0.2788 -0.0383 0.0622 0.4085 -0.1544 0.2926 1.9312 5.9726 53.5137 16 0.3812 -0.2537 0.0052 0.6069 -0.1021 0.0289 0.0626 -0.0563 -0.1421 0.1714 -0.0175 0.2155 0.2963 0.1258 0.0654 0.3428 0.0277 0.03 0.6506 -0.2268 0.2108 10.1213 1.1329 44.1097 17 0.402 -0.0528 -0.0304 0.262 -0.0206 0.0128 -0.0191 0.6027 0.0824 -0.3062 -0.153 0.0963 -0.1916 -0.1545 0.0413 0.4869 0.0979 -0.1306 0.6861 -0.0422 0.161 2.4817 16.7135 43.7065 18 0.0709 -0.159 -0.1285 0.5468 -0.0943 0.8408 0.0285 0.307 -0.29 -0.1424 -0.3033 0.1548 0.1992 0.1124 0.1142 0.4442 0.0785 0.0012 0.3396 -0.0165 0.2877 16.2698 -2.6786 65.9996 19 0.1714 0.0638 -0.1997 0.2988 0.2828 0.7235 -0.0126 -0.0108 0.0605 -0.0343 -0.0241 -0.0272 -0.0556 0.001 0.0135 1.4381 -0.2622 -0.0064 1.0847 -0.2711 0.8295 21.6497 10.383 13.4934
精密化 TLSグループ 大きな表を表示 (4 x 19) 大きな表を隠す ID Refine-ID Refine TLS-ID Selection details 1 X-RAY DIFFRACTION 1 chain 'A' and (resseq 5:18)2 X-RAY DIFFRACTION 2 chain 'A' and (resseq 19:41)3 X-RAY DIFFRACTION 3 chain 'A' and (resseq 42:71)4 X-RAY DIFFRACTION 4 chain 'A' and (resseq 72:109)5 X-RAY DIFFRACTION 5 chain 'A' and (resseq 110:122)6 X-RAY DIFFRACTION 6 chain 'A' and (resseq 123:136)7 X-RAY DIFFRACTION 7 chain 'A' and (resseq 137:163)8 X-RAY DIFFRACTION 8 chain 'A' and (resseq 164:182)9 X-RAY DIFFRACTION 9 chain 'A' and (resseq 183:197)10 X-RAY DIFFRACTION 10 chain 'A' and (resseq 198:217)11 X-RAY DIFFRACTION 11 chain 'B' and (resseq 5:18)12 X-RAY DIFFRACTION 12 chain 'B' and (resseq 19:41)13 X-RAY DIFFRACTION 13 chain 'B' and (resseq 42:71)14 X-RAY DIFFRACTION 14 chain 'B' and (resseq 72:150)15 X-RAY DIFFRACTION 15 chain 'B' and (resseq 151:182)16 X-RAY DIFFRACTION 16 chain 'B' and (resseq 183:197)17 X-RAY DIFFRACTION 17 chain 'B' and (resseq 198:217)18 X-RAY DIFFRACTION 18 chain 'C'19 X-RAY DIFFRACTION 19