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- PDB-4lgn: The structure of Acidothermus cellulolyticus family 74 glycoside ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4lgn | ||||||
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Title | The structure of Acidothermus cellulolyticus family 74 glycoside hydrolase | ||||||
![]() | Cellulose-binding, family II | ||||||
![]() | CELLULOSE BINDING PROTEIN / GH74 / xyloglucanase | ||||||
Function / homology | ![]() cellulose binding / hydrolase activity, hydrolyzing O-glycosyl compounds / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Alahuhta, P.M. / Lunin, V.V. | ||||||
![]() | ![]() Title: Structure of Acidothermus cellulolyticus family 74 glycoside hydrolase at 1.82 angstrom resolution. Authors: Alahuhta, M. / Adney, W.S. / Himmel, M.E. / Lunin, V.V. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 189.7 KB | Display | ![]() |
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PDB format | ![]() | 143.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2cn3S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 79478.406 Da / Num. of mol.: 1 / Fragment: UNP residues 47-786 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 7 types, 1157 molecules ![](data/chem/img/K.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/FMT.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/FMT.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-GOL / #5: Chemical | #6: Chemical | ChemComp-NA / #7: Chemical | ChemComp-FMT / #8: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.82 % |
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Crystal grow | Temperature: 294 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.1 M tri-Sodium citrate Dihydrate pH 5.0, 1.5 M Na Formate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: Bruker Platinum 135 / Detector: CCD / Date: Mar 5, 2010 / Details: HELIOS MIRRORS |
Radiation | Monochromator: HELIOS MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.82→40.41 Å / Num. all: 76875 / Num. obs: 76875 / % possible obs: 100 % / Redundancy: 5.66 % / Rsym value: 0.1557 / Net I/σ(I): 8.52 |
Reflection shell | Resolution: 1.82→1.91 Å / Redundancy: 4.34 % / Mean I/σ(I) obs: 1.98 / Num. unique all: 10202 / Rsym value: 0.5343 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB entry 2CN3 Resolution: 1.82→40.41 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.931 / SU B: 2.491 / SU ML: 0.074 / Cross valid method: THROUGHOUT / ESU R: 0.109 / ESU R Free: 0.112 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.469 Å2
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Refinement step | Cycle: LAST / Resolution: 1.82→40.41 Å
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Refine LS restraints |
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