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- PDB-4ld7: Crystal structure of AnaPT from Neosartorya fischeri -

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Basic information

Entry
Database: PDB / ID: 4ld7
TitleCrystal structure of AnaPT from Neosartorya fischeri
ComponentsDimethylallyl tryptophan synthase
KeywordsTRANSFERASE / ABBA-prenyltransferase / prenyltransferase
Function / homologyAromatic prenyltransferase DMATS-type, fungi / Aromatic prenyltransferase, DMATS-type / Tryptophan dimethylallyltransferase / Aromatic prenyltransferase / alkaloid metabolic process / Transferases; Transferring alkyl or aryl groups, other than methyl groups / transferase activity, transferring alkyl or aryl (other than methyl) groups / TRIHYDROGEN THIODIPHOSPHATE / Indole diterpene prenyltransferase anaPT
Function and homology information
Biological speciesNeosartorya fischeri (mold)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.83 Å
AuthorsZocher, G. / Stehle, T.
CitationJournal: Chem.Biol. / Year: 2013
Title: Catalytic Mechanism of Stereospecific Formation of cis-Configured Prenylated Pyrroloindoline Diketopiperazines by Indole Prenyltransferases.
Authors: Yu, X. / Zocher, G. / Xie, X. / Liebhold, M. / Schutz, S. / Stehle, T. / Li, S.M.
History
DepositionJun 24, 2013Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 11, 2013Provider: repository / Type: Initial release
Revision 1.1Jan 15, 2014Group: Database references
Revision 1.2Nov 15, 2017Group: Refinement description / Category: software / Item: _software.name
Revision 1.3Feb 28, 2024Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ncs_dom_lim / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_label_asym_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Dimethylallyl tryptophan synthase
B: Dimethylallyl tryptophan synthase
C: Dimethylallyl tryptophan synthase
D: Dimethylallyl tryptophan synthase
E: Dimethylallyl tryptophan synthase
F: Dimethylallyl tryptophan synthase
G: Dimethylallyl tryptophan synthase
H: Dimethylallyl tryptophan synthase
I: Dimethylallyl tryptophan synthase
J: Dimethylallyl tryptophan synthase
K: Dimethylallyl tryptophan synthase
L: Dimethylallyl tryptophan synthase
M: Dimethylallyl tryptophan synthase
N: Dimethylallyl tryptophan synthase
O: Dimethylallyl tryptophan synthase
P: Dimethylallyl tryptophan synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)802,32848
Polymers798,87116
Non-polymers3,45632
Water0
1
A: Dimethylallyl tryptophan synthase
B: Dimethylallyl tryptophan synthase
C: Dimethylallyl tryptophan synthase
D: Dimethylallyl tryptophan synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,58212
Polymers199,7184
Non-polymers8648
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10940 Å2
ΔGint-90 kcal/mol
Surface area57090 Å2
MethodPISA
2
E: Dimethylallyl tryptophan synthase
F: Dimethylallyl tryptophan synthase
G: Dimethylallyl tryptophan synthase
H: Dimethylallyl tryptophan synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,58212
Polymers199,7184
Non-polymers8648
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10850 Å2
ΔGint-91 kcal/mol
Surface area57230 Å2
MethodPISA
3
I: Dimethylallyl tryptophan synthase
J: Dimethylallyl tryptophan synthase
K: Dimethylallyl tryptophan synthase
L: Dimethylallyl tryptophan synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,58212
Polymers199,7184
Non-polymers8648
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10690 Å2
ΔGint-91 kcal/mol
Surface area57300 Å2
MethodPISA
4
M: Dimethylallyl tryptophan synthase
N: Dimethylallyl tryptophan synthase
O: Dimethylallyl tryptophan synthase
P: Dimethylallyl tryptophan synthase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)200,58212
Polymers199,7184
Non-polymers8648
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10700 Å2
ΔGint-89 kcal/mol
Surface area57160 Å2
MethodPISA
Unit cell
Length a, b, c (Å)97.550, 242.590, 145.320
Angle α, β, γ (deg.)90.00, 89.99, 90.00
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11A
21B
31C
41D
51E
61F
71G
81H
91I
101J
111K
121L
131M
141N
151O
161P

NCS domain segments:

Component-ID: 1 / Ens-ID: 1 / Beg auth comp-ID: GLN / Beg label comp-ID: GLN / End auth comp-ID: LEU / End label comp-ID: LEU / Refine code: 2 / Auth seq-ID: 30 - 437 / Label seq-ID: 30 - 437

Dom-IDAuth asym-IDLabel asym-ID
1AA
2BB
3CC
4DD
5EE
6FF
7GG
8HH
9II
10JJ
11KK
12LL
13MM
14NN
15OO
16PP

NCS oper:
IDCodeMatrixVector
1given(1), (1), (1)
2given(0.934208, 0.306836, -0.181952), (0.304819, -0.951584, -0.039662), (-0.185312, -0.01841, -0.982507)19.7518, 40.01717, 277.72906
3given(-0.944693, -0.325869, 0.03694), (-0.32638, 0.923143, -0.203182), (0.03211, -0.204001, -0.978444)-98.84131, 13.66958, 289.55301
4given(-0.990098, 0.007384, 0.140187), (0.024278, -0.974561, 0.222805), (0.138266, 0.224003, 0.964731)-119.07968, -11.15963, 9.58657
5given(0.989475, -0.005949, 0.144579), (-0.027482, 0.973244, 0.228126), (-0.142068, -0.229699, 0.962837)11.40852, -86.08651, -67.39699
6given(0.94365, 0.329667, 0.029052), (0.328431, -0.922068, -0.204756), (-0.040713, 0.202759, -0.978382)16.71762, 126.56453, 343.96918
7given(-0.934063, -0.311764, -0.174153), (-0.309285, 0.950046, -0.041906), (0.178519, 0.01472, -0.983826)-84.80222, -53.34522, 374.2482
8given(-0.999865, 0.01617, 0.003081), (-0.016167, -0.999869, 0.00108), (0.003098, 0.00103, 0.999995)-144.27902, 53.44622, -72.4449
9given(0.972967, -0.2206, -0.068346), (0.223119, 0.974278, 0.031633), (0.059609, -0.046027, 0.99716)28.04888, -52.94653, 7.55554
10given(0.964441, 0.13786, -0.225496), (0.120342, -0.988668, -0.089737), (-0.235311, 0.059409, -0.970103)24.15063, 99.68855, 267.00754
11given(-0.984949, -0.148371, 0.088666), (-0.161653, 0.972328, -0.168664), (-0.061187, -0.180458, -0.981678)-104.63418, -44.63466, 295.20413
12given(-0.957355, 0.2121, 0.196174), (-0.172202, -0.964123, 0.202024), (0.231985, 0.159627, 0.959532)-144.55006, 40.61032, 7.4457
13given(-0.974917, 0.215109, -0.05713), (-0.217253, -0.975511, 0.034353), (-0.048341, 0.045903, 0.997776)-127.33266, -34.84115, -75.23924
14given(-0.965949, -0.144581, -0.214566), (-0.125201, 0.986937, -0.101389), (0.226422, -0.071073, -0.971433)-98.26997, 35.55183, 375.15887
15given(0.984578, 0.154515, 0.08204), (0.166078, -0.972928, -0.160716), (0.054986, 0.171863, -0.983585)29.20997, 44.29238, 365.21396
16given(0.962298, -0.191581, 0.193079), (0.153062, 0.96821, 0.197842), (-0.224844, -0.16083, 0.96103)-2.64001, -2.16622, -87.61595

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Components

#1: Protein
Dimethylallyl tryptophan synthase / AnaPT


Mass: 49929.457 Da / Num. of mol.: 16
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Neosartorya fischeri (mold) / Gene: NFIA_055300 / Production host: Escherichia coli (E. coli)
References: UniProt: A1DN10, 4-dimethylallyltryptophan synthase
#2: Chemical
ChemComp-PIS / TRIHYDROGEN THIODIPHOSPHATE / THIOPYROPHOSPHATE


Mass: 193.033 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: H3O6P2S
#3: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 16 / Source method: obtained synthetically / Formula: Na

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.15 Å3/Da / Density % sol: 42.85 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5
Details: 100 mM MES/imidazole, pH 6.5, 18-22% v/v ethylene glycol, 9-11% w/v PEG8000, 80-160 mM alcohol mix, VAPOR DIFFUSION, SITTING DROP, temperature 293K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.99996 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 17, 2013
RadiationMonochromator: double crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.99996 Å / Relative weight: 1
Reflection twin
Crystal-IDIDOperatorDomain-IDFraction
11H, K, L10.544
11-h,-k,l20.456
ReflectionResolution: 2.83→40 Å / Num. all: 160059 / Num. obs: 160059 / % possible obs: 99.8 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2
Reflection shellResolution: 2.83→2.9 Å / % possible all: 99.9

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Processing

Software
NameVersionClassification
RemDAqdata collection
PHASERphasing
REFMAC5.8.0044refinement
XDSdata reduction
XSCALEdata scaling
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.83→39.95 Å / Cor.coef. Fo:Fc: 0.923 / Cor.coef. Fo:Fc free: 0.931 / SU B: 24.046 / SU ML: 0.233 / Cross valid method: THROUGHOUT / ESU R Free: 0.069 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
RfactorNum. reflection% reflectionSelection details
Rfree0.20211 3191 2 %RANDOM
Rwork0.20252 ---
obs0.20251 156867 99.84 %-
all-160059 --
Solvent computationIon probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK
Displacement parametersBiso mean: 61.058 Å2
Baniso -1Baniso -2Baniso -3
1--21.65 Å2-0 Å217.18 Å2
2--6.66 Å20 Å2
3---14.99 Å2
Refinement stepCycle: LAST / Resolution: 2.83→39.95 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms48819 0 160 0 48979
Refine LS restraints
Refine-IDTypeDev idealDev ideal targetNumber
X-RAY DIFFRACTIONr_bond_refined_d0.0050.02250207
X-RAY DIFFRACTIONr_bond_other_d
X-RAY DIFFRACTIONr_angle_refined_deg0.8491.9668489
X-RAY DIFFRACTIONr_angle_other_deg
X-RAY DIFFRACTIONr_dihedral_angle_1_deg4.08256307
X-RAY DIFFRACTIONr_dihedral_angle_2_deg34.64923.7092076
X-RAY DIFFRACTIONr_dihedral_angle_3_deg13.93157580
X-RAY DIFFRACTIONr_dihedral_angle_4_deg17.54615221
X-RAY DIFFRACTIONr_chiral_restr0.0530.27682
X-RAY DIFFRACTIONr_gen_planes_refined0.0030.02138205
X-RAY DIFFRACTIONr_gen_planes_other
X-RAY DIFFRACTIONr_nbd_refined
X-RAY DIFFRACTIONr_nbd_other
X-RAY DIFFRACTIONr_nbtor_refined
X-RAY DIFFRACTIONr_nbtor_other
X-RAY DIFFRACTIONr_xyhbond_nbd_refined
X-RAY DIFFRACTIONr_xyhbond_nbd_other
X-RAY DIFFRACTIONr_metal_ion_refined
X-RAY DIFFRACTIONr_metal_ion_other
X-RAY DIFFRACTIONr_symmetry_vdw_refined
X-RAY DIFFRACTIONr_symmetry_vdw_other
X-RAY DIFFRACTIONr_symmetry_hbond_refined
X-RAY DIFFRACTIONr_symmetry_hbond_other
X-RAY DIFFRACTIONr_symmetry_metal_ion_refined
X-RAY DIFFRACTIONr_symmetry_metal_ion_other
X-RAY DIFFRACTIONr_mcbond_it0.4323.55125330
X-RAY DIFFRACTIONr_mcbond_other
X-RAY DIFFRACTIONr_mcangle_it0.7775.98931603
X-RAY DIFFRACTIONr_mcangle_other
X-RAY DIFFRACTIONr_scbond_it0.3993.52824877
X-RAY DIFFRACTIONr_scbond_other
X-RAY DIFFRACTIONr_scangle_it
X-RAY DIFFRACTIONr_scangle_other
X-RAY DIFFRACTIONr_long_range_B_refined2.63613.7772038
X-RAY DIFFRACTIONr_long_range_B_other
X-RAY DIFFRACTIONr_rigid_bond_restr
X-RAY DIFFRACTIONr_sphericity_free
X-RAY DIFFRACTIONr_sphericity_bonded
Refine LS restraints NCS

Ens-ID: 1 / Refine-ID: X-RAY DIFFRACTION

Dom-IDAuth asym-IDNumberTypeRms dev position (Å)Weight position
1A1425MEDIUM POSITIONAL0.010.5
2B1425MEDIUM POSITIONAL0.010.5
3C1425MEDIUM POSITIONAL0.010.5
4D1425MEDIUM POSITIONAL0.010.5
5E1425MEDIUM POSITIONAL0.010.5
6F1425MEDIUM POSITIONAL0.010.5
7G1425MEDIUM POSITIONAL0.010.5
8H1425MEDIUM POSITIONAL0.010.5
9I1425MEDIUM POSITIONAL0.5
10J1425MEDIUM POSITIONAL0.010.5
11K1425MEDIUM POSITIONAL0.5
12L1425MEDIUM POSITIONAL0.5
13M1425MEDIUM POSITIONAL0.5
14N1425MEDIUM POSITIONAL0.010.5
15O1425MEDIUM POSITIONAL0.5
16P1425MEDIUM POSITIONAL0.5
1A1560TIGHT THERMAL4.080.5
2B1560TIGHT THERMAL2.870.5
3C1560TIGHT THERMAL4.810.5
4D1560TIGHT THERMAL3.170.5
5E1560TIGHT THERMAL2.890.5
6F1560TIGHT THERMAL2.630.5
7G1560TIGHT THERMAL2.920.5
8H1560TIGHT THERMAL2.80.5
9I1560TIGHT THERMAL3.210.5
10J1560TIGHT THERMAL3.220.5
11K1560TIGHT THERMAL3.630.5
12L1560TIGHT THERMAL3.040.5
13M1560TIGHT THERMAL3.610.5
14N1560TIGHT THERMAL3.160.5
15O1560TIGHT THERMAL3.060.5
16P1560TIGHT THERMAL4.40.5
1A1425MEDIUM THERMAL4.062
2B1425MEDIUM THERMAL2.982
3C1425MEDIUM THERMAL4.932
4D1425MEDIUM THERMAL3.32
5E1425MEDIUM THERMAL3.152
6F1425MEDIUM THERMAL2.762
7G1425MEDIUM THERMAL2.982
8H1425MEDIUM THERMAL2.742
9I1425MEDIUM THERMAL3.292
10J1425MEDIUM THERMAL3.182
11K1425MEDIUM THERMAL3.682
12L1425MEDIUM THERMAL3.12
13M1425MEDIUM THERMAL3.672
14N1425MEDIUM THERMAL3.142
15O1425MEDIUM THERMAL3.152
16P1425MEDIUM THERMAL4.322
LS refinement shellResolution: 2.83→2.903 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.304 230 -
Rwork0.306 11445 -
obs--99.23 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.0481-0.1657-0.57670.7746-0.08042.54730.0111-0.2787-0.10290.0685-0.0093-0.10020.22550.3235-0.00180.20010.0199-0.14590.09070.01480.1223-25.39521.012160.2803
21.99360.1970.32760.9533-0.19992.17820.00760.26250.3938-0.1228-0.0808-0.109-0.18210.19720.07320.2193-0.0402-0.11910.07550.10260.2152-32.29625.4167125.1696
31.98210.0962-0.7191.10590.01371.9352-0.13560.3211-0.3384-0.10210.00780.12890.2568-0.27350.12780.2898-0.0538-0.16480.0751-0.05040.2038-69.4108-9.2472131.7643
42.57260.12250.02450.48580.19091.93560.0498-0.22910.4602-0.0136-0.05980.1037-0.1806-0.11180.00990.17560.0283-0.13420.0655-0.08230.232-71.593522.6182161.2272
52.00220.2442-0.40850.5541-0.3311.6178-0.0572-0.1342-0.40890.02040.052-0.10390.21150.1890.00520.26950.002-0.1950.11460.06360.2616-71.221932.6577233.7618
61.64130.28240.62961.3817-0.07082.6065-0.08570.26810.2164-0.1990.053-0.2335-0.57770.30960.03270.466-0.1232-0.08290.07730.0520.1437-73.515564.6384204.1851
71.97870.4862-0.38481.10530.15492.1383-0.05250.2221-0.3223-0.0829-0.01560.08510.1492-0.33970.06810.26020-0.1610.1012-0.06540.1821-110.507329.9638197.8722
82.14830.01010.18290.6827-0.0232.92330.0516-0.16130.2097-0.0049-0.04950.1063-0.2424-0.4345-0.0020.24270.0399-0.1250.1063-0.02260.1144-117.308154.5892233.0295
92.0726-0.6066-0.44741.20650.45241.6122-0.0537-0.5604-0.54280.4638-0.1027-0.17070.25720.08250.15640.6173-0.0468-0.25960.31160.26020.5792-30.856957.3216157.6574
101.83040.050.46151.4763-0.13521.91030.07840.30250.0528-0.1907-0.0991-0.2755-0.12230.12450.02070.33920.0098-0.1590.07830.01460.2584-34.544984.3912123.5546
111.77280.2987-0.87740.987-0.14641.8541-0.08340.3563-0.3346-0.12670.10730.43790.2858-0.3859-0.02390.473-0.1029-0.20130.2976-0.0720.7356-76.911157.1158131.6206
122.1318-0.7465-0.24580.8496-0.5322.19560.1016-0.52420.23180.69230.21190.3411-0.8670.1761-0.31351.1554-0.05860.22030.3468-0.0040.7119-71.799787.8505162.0302
131.71790.02560.7591.4031-0.1111.8371-0.0484-0.52090.5320.3409-0.00840.3475-0.1224-0.20830.05680.6301-0.0088-0.17750.4487-0.19690.5651-118.5121-2.267230.3828
142.4740.1839-0.04981.24570.06991.53410.01850.13930.00420.01840.0434-0.01350.17030.0686-0.06190.47310.0134-0.28970.18630.00670.1888-114.7285-29.3567196.5965
150.77840.07530.12610.91730.04611.70990.00510.17990.34410.0026-0.1003-0.3764-0.09880.4560.09510.53210.0726-0.25990.46410.01860.6371-72.4716-1.7322203.9273
161.8057-1.0255-0.30541.011-0.24032.42410.0357-0.1889-0.1759-0.0634-0.012-0.05130.413-0.201-0.02360.63830.0381-0.36560.3894-0.07650.3453-77.1514-32.4356234.4686
Refinement TLS group
IDRefine-IDRefine TLS-IDAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1A30 - 437
2X-RAY DIFFRACTION2B30 - 437
3X-RAY DIFFRACTION3C30 - 437
4X-RAY DIFFRACTION4D30 - 437
5X-RAY DIFFRACTION5E30 - 437
6X-RAY DIFFRACTION6F30 - 437
7X-RAY DIFFRACTION7G30 - 437
8X-RAY DIFFRACTION8H30 - 437
9X-RAY DIFFRACTION9I30 - 437
10X-RAY DIFFRACTION10J30 - 437
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