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基本情報
登録情報 | データベース: PDB / ID: 4j6s | ||||||
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タイトル | 14-3-3gamma complexed with the N-terminal sequence of tyrosine hydroxylase (residues 1-43) | ||||||
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![]() | HYDROLASE / 14-3-3 proteins / peptide binding / Dopamine synthesis / signal transduction / regulatory proteins / tyrosine hydroxylase / phosphorylation | ||||||
機能・相同性 | ![]() tyrosine 3-monooxygenase / tyrosine 3-monooxygenase activity / phytoalexin metabolic process / dopamine biosynthetic process from tyrosine / phthalate metabolic process / glycoside metabolic process / terpene metabolic process / isoquinoline alkaloid metabolic process / norepinephrine biosynthetic process / embryonic camera-type eye morphogenesis ...tyrosine 3-monooxygenase / tyrosine 3-monooxygenase activity / phytoalexin metabolic process / dopamine biosynthetic process from tyrosine / phthalate metabolic process / glycoside metabolic process / terpene metabolic process / isoquinoline alkaloid metabolic process / norepinephrine biosynthetic process / embryonic camera-type eye morphogenesis / hyaloid vascular plexus regression / epinephrine biosynthetic process / circadian sleep/wake cycle / Catecholamine biosynthesis / aminergic neurotransmitter loading into synaptic vesicle / dopamine binding / response to pyrethroid / eye photoreceptor cell development / response to isolation stress / melanosome membrane / sphingolipid metabolic process / response to ether / synaptic transmission, dopaminergic / tetrahydrobiopterin binding / response to herbicide / mating behavior / dopamine biosynthetic process / regulation of neuron differentiation / amino acid binding / regulation of heart contraction / eating behavior / pigmentation / response to corticosterone / response to zinc ion / protein kinase C inhibitor activity / cellular response to alkaloid / social behavior / Regulation of localization of FOXO transcription factors / response to immobilization stress / phosphoserine residue binding / smooth endoplasmic reticulum / Activation of BAD and translocation to mitochondria / response to light stimulus / regulation of signal transduction / anatomical structure morphogenesis / cellular response to manganese ion / protein targeting / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / cellular response to glucose starvation / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / response to electrical stimulus / heart morphogenesis / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / negative regulation of TORC1 signaling / Recruitment of NuMA to mitotic centrosomes / response to salt stress / Anchoring of the basal body to the plasma membrane / protein sequestering activity / response to amphetamine / visual perception / AURKA Activation by TPX2 / response to nutrient levels / ferric iron binding / fatty acid metabolic process / learning / response to activity / locomotory behavior / Translocation of SLC2A4 (GLUT4) to the plasma membrane / cellular response to glucose stimulus / TP53 Regulates Metabolic Genes / protein kinase C binding / animal organ morphogenesis / insulin-like growth factor receptor binding / negative regulation of protein kinase activity / regulation of synaptic plasticity / ferrous iron binding / terminal bouton / cytoplasmic side of plasma membrane / receptor tyrosine kinase binding / cerebral cortex development / memory / cellular response to growth factor stimulus / response to peptide hormone / oxygen binding / cellular response to nicotine / cellular response to insulin stimulus / Regulation of PLK1 Activity at G2/M Transition / cellular response to xenobiotic stimulus / synaptic vesicle / presynapse / response to estradiol / heart development / cytoplasmic vesicle / perikaryon / response to ethanol / response to lipopolysaccharide 類似検索 - 分子機能 | ||||||
生物種 | ![]() | ||||||
手法 | ![]() ![]() ![]() | ||||||
![]() | Mileni, M. / Martinez, A. / Stevens, R.C. | ||||||
![]() | ![]() タイトル: The N-terminal sequence of tyrosine hydroxylase is a conformationally versatile motif that binds 14-3-3 proteins and membranes. 著者: Skjevik, A.A. / Mileni, M. / Baumann, A. / Halskau, O. / Teigen, K. / Stevens, R.C. / Martinez, A. | ||||||
履歴 |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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PDBx/mmCIF形式 | ![]() | 204.5 KB | 表示 | ![]() |
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PDB形式 | ![]() | 164.2 KB | 表示 | ![]() |
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その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 482.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 490.9 KB | 表示 | |
XML形式データ | ![]() | 33.1 KB | 表示 | |
CIF形式データ | ![]() | 45.3 KB | 表示 | |
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-関連構造データ
関連構造データ | ![]() 1a4oS S: 精密化の開始モデル |
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類似構造データ |
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リンク
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集合体
登録構造単位 | ![]()
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単位格子 |
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非結晶学的対称性 (NCS) | NCS oper:
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要素
#1: タンパク質 | 分子量: 29165.545 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() ![]() #2: タンパク質・ペプチド | 分子量: 4859.459 Da / 分子数: 4 / 由来タイプ: 合成 / 由来: (合成) ![]() 配列の詳細 | THE CRYSTALLIZED SEQUENCE CORRESPONDING TO TYROSINE 3-MONOOXYGENASE REPRESENTS THE SPLICING-ISOFORM ...THE CRYSTALLIZ | |
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-実験情報
-実験
実験 | 手法: ![]() |
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試料調製
結晶 | マシュー密度: 2.44 Å3/Da / 溶媒含有率: 49.53 % |
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結晶化 | 温度: 283 K / 手法: 蒸気拡散法 / pH: 7.1 詳細: 30% PEG 2000 MME, 0.1M Potassium Thiocyanate, pH 7.1, VAPOR DIFFUSION, temperature 283.0K |
-データ収集
回折 | 平均測定温度: 100 K |
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放射光源 | 由来: ![]() ![]() ![]() |
検出器 | タイプ: MARMOSAIC 300 mm CCD / 検出器: CCD / 日付: 2008年10月15日 |
放射 | モノクロメーター: Si(111) / プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1.0331 Å / 相対比: 1 |
反射 | 解像度: 3.08→30 Å / Num. all: 22015 / Num. obs: 111792 / % possible obs: 87.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / 冗長度: 5.1 % / Rmerge(I) obs: 0.111 / Net I/σ(I): 11.6 |
反射 シェル | 解像度: 3.08→3.16 Å / 冗長度: 5.1 % / Rmerge(I) obs: 0.492 / Mean I/σ(I) obs: 3.29 / Num. unique all: 1656 / % possible all: 90 |
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解析
ソフトウェア |
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精密化 | 構造決定の手法: ![]() 開始モデル: PDB ENTRY 1A4O 解像度: 3.08→29.9 Å / Cor.coef. Fo:Fc: 0.93 / Cor.coef. Fo:Fc free: 0.899 / Isotropic thermal model: isotropic / 交差検証法: THROUGHOUT / ESU R Free: 0.514 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 68.709 Å2
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精密化ステップ | サイクル: LAST / 解像度: 3.08→29.9 Å
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拘束条件 |
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LS精密化 シェル | 解像度: 3.08→3.16 Å / Total num. of bins used: 20
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