[English] 日本語
![](img/lk-miru.gif)
- PDB-4irm: Crystal structure of mntc r116a mutant exhibits flexibility in th... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 4irm | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Title | Crystal structure of mntc r116a mutant exhibits flexibility in the c-terminal domain | |||||||||
![]() | Mn transporter; MntC | |||||||||
![]() | ![]() ![]() | |||||||||
Function / homology | ![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Kanteev, M. / Adir, N. | |||||||||
![]() | ![]() Title: Arginine 116 stabilizes the entrance to the metal ion-binding site of the MntC protein. Authors: Kanteev, M. / Adir, N. | |||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 160.6 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 125.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 3ujpSC S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 36014.133 Da / Num. of mol.: 3 / Mutation: R116A Source method: isolated from a genetically manipulated source Details: includes TEV cleavage site / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.15 % |
---|---|
Crystal grow![]() | Temperature: 290 K / pH: 6.5 Details: PEG 4000, SODIUM CACODYLATE, 50mM ZINC CHLORIDE,, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.5→24.5 Å / Num. obs: 11028 / % possible obs: 99 % / Redundancy: 5.3 % / Rmerge(I) obs: 0.078 / Net I/σ(I): 15 |
Reflection shell | Resolution: 3.5→3.69 Å / Redundancy: 4.9 % / Mean I/σ(I) obs: 6.2 |
-
Processing
Software |
| |||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3UJP Resolution: 3.5→24.5 Å / Isotropic thermal model: Isotropic / Phase error: 35.89 / Stereochemistry target values: TWIN_LSQ_F
| |||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 64.19 Å2 / ksol: 0.24 e/Å3 | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 97.5 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.5→24.5 Å
| |||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||
LS refinement shell |
|