Replication initiation factor / Replication initiation factor / DNA topoisomerase activity / DNA replication initiation / DNA replication / DNA binding / Replication initiation protein / Replication initiation protein
PROTEIN WAS CONSTRUCTED BY FUSING THE N-TERMINAL DOMAIN OF REPD - BASES 1295-1849 FROM PLASMID ...PROTEIN WAS CONSTRUCTED BY FUSING THE N-TERMINAL DOMAIN OF REPD - BASES 1295-1849 FROM PLASMID PC221 (GENBANK ACCESSION X02166) WITH THE C-TERMINAL DOMAIN OF REPN - BASES 856-1143 FROM PLASMID PCW7 (GENBANK ACCESSION J03323)
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実験情報
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実験
実験
手法: X線回折 / 使用した結晶の数: 1
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試料調製
結晶
マシュー密度: 2.92 Å3/Da / 溶媒含有率: 57.8 % / 解説: NONE
結晶化
pH: 5.5 / 詳細: 0.1 M SODIUM CITRATE PH 5.5, 2.5 M 1,6-HEXANEDIOL
解像度: 3→39.66 Å / Cor.coef. Fo:Fc: 0.891 / Cor.coef. Fo:Fc free: 0.844 / SU B: 19.145 / SU ML: 0.376 / 交差検証法: THROUGHOUT / ESU R Free: 0.106 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS. U VALUES REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.29711
770
4.8 %
RANDOM
Rwork
0.24106
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obs
0.24364
15210
99.81 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK